Faecal contamination and prevalence of pathogenic E. coli in shellfish growing areas along south-west coast of India

Antony, Ally C.; Silvester, Reshma; Divya, P S; Aneesa, P A; Francis, Bini; Joseph, C. Ajith; Hussain, Midhun Shah; Umesh, B T; George, Jino; Hatha, A. A. Mohamed

doi:10.1016/j.rsma.2021.101774

Cited by 5 publications

(4 citation statements)

References 48 publications

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“…There are reports on the occurrence of EHEC O157 strains from shellfish and sediment samples from Cochin estuary (Antony et al, 2021) and the Indian coastal belts (Sehgal et al, 2008). Our results, however, reveal a relatively low prevalence of E. coli O157 serotype in the biofilm samples from the seafood contact surfaces.…”

Section: Discussioncontrasting

confidence: 69%

“…Our results, however, reveal a relatively low prevalence of E. coli O157 serotype in the biofilm samples from the seafood contact surfaces. The presence of EHEC O157 necessitates urgent attention since it is one of the most potential pathogens responsible for severe diseases that results in hospitalizations and fatalities (Antony et al, 2021). Approximately 6.67% of the E. coli strains in the current study were not typeable, which indicates the presence of either novel or unrecognized O-antigen which do not react with the currently available 'O' antisera.…”

Section: Discussionmentioning

confidence: 73%

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Prevalence, Antimicrobial Resistance, and Molecular Characterization of Escherichia coli Isolated from Food Contact Surfaces in Seafood Pre-Processing Plants (India)

Francis¹,

Antony²,

Sukumaran³

et al. 2022

JFQHC

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Background: The survival of pathogens in biofilms poses a threat to food safety. The aim of this study was to determine prevalence, antimicrobial resistance, and molecular characterization of Escherichia coli strains. Methods: Swab samples (n=144) were collected from biofilm formed on food contact surfaces in seafood pre-processing plant in India. E. coli was isolated and identified using uid A gene by Polymerase Chain Reaction (PCR). The risk assessment of the isolates was carried out in terms of their drug resistance and the presence of virulence genes. Antimicrobial susceptibility testing was performed using the Kirby-Bauer disk diffusion method. Phylogenetic grouping was done by quadruplex PCR. Molecular typing of the strains was performed by Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR). Data were statistically analyzed using SPSS version 22. Results: Enteropathogenic E. coli (EPEC) strains were the most prevalent serotype. Multiplex PCR analysis revealed the presence of shiga toxin genes (stx1, stx2), intimin (eae), and enterohemolysin genes (hlyA). Shiga toxin gene stx2 showed the highest prevalence (83.33%). Among various phylogroups, B1 (45.56%) and B2 (30%) were the most prevalent phylogroups. Resistance to ampicillin (85.56%), piperacillin (84.44%), and cefpodoxime (85.56%) was widespread among the E. coli strains. Conclusion: The presence of genetically heterogeneous multi drug resistant E. coli strains with virulence potential showed a high risk in the seafood industry.

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Section: Discussioncontrasting

confidence: 69%

Section: Discussionmentioning

confidence: 73%

Prevalence, Antimicrobial Resistance, and Molecular Characterization of Escherichia coli Isolated from Food Contact Surfaces in Seafood Pre-Processing Plants (India)

Francis¹,

Antony²,

Sukumaran³

et al. 2022

JFQHC

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“…However, oysters are an important medium for infecting humans with these pathogens as they are eaten raw or partially cooked [12]. Several studies have been conducted worldwide to determine the bacterial prevalence in shellfsh-growing waters [13][14][15]. To date, similar studies have not been conducted in Saldanha Bay except for the microbiological monitoring undertaken by the South African Department of Forestry, Fisheries, and Environment.…”

Section: Introductionmentioning

confidence: 99%

The Prevalence of Bacteria Commonly Related to the Production of Mussels and Oysters in Saldanha Bay

Shuping

Human

Lues

et al. 2023

Aquaculture Research

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Bivalve molluscs are a good source of high quality protein and perform important ecological functions. Their ability to bioaccumulate materials in their soft tissues makes them suitable aquatic species for biomonitoring of environmental conditions. The discharge of treated and untreated sewage into the bivalve-growing areas is a concern. The aim of this study was to investigate the prevalence of bacterial microbiota in shellfish farms in Saldanha Bay harbour using pathogens commonly associated with shellfish-related foodborne disease outbreaks. Seawater and mussel samples were collected from five sampling points located in three sampling locations. Oyster samples were collected from the harbour deck immediately after harvesting by the farmers. The most probable number (MPN) method was used to enumerate E. coli and faecal coliforms. Cultural methods were used for the detection of Salmonella and Vibrio spp. The E. coli concentrations for 15 March and 14 July are <0.18 MPN/100 ml at all sampling sites and for 25 August, <0.18 MPN/100 ml for all sampling sites except sampling site SP2 (0.2 MPN/100 ml). Spikes were observed on the total MPN counts in winter. Salmonella and Vibrio spp. were not detected. However, other bacterial species were identified through their phenotypic profile using the VITEK 2 system. Based on the low E. coli-MPN concentrations, the study concluded that the molluscs were safe for human consumption. Further studies need to be conducted on the bacterial species identified.

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“…The abuse of traditional antibiotics has exacerbated the selective evolution of bacteria and poses a great threat to human health [ 1 , 2 ]. Nowadays, the advent of nanomaterials and their introduction into the biological field for the treatment of multiple diseases has provided new ideas for the development of superior antibacterial agents [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of an Antibrowning Nanosized Ag-CeO2 Composite with Synergistic Antibacterial Ability

et al. 2023

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Nanosized Ag and CeO2 particles obtained through the hydrothermal method were physically mixed to obtain composite antibacterial agents. The comparative experiments of antibacterial properties showed that the antibacterial activity of the nanocomposites was improved compared to the nanoparticles alone, which indicated that the synergistic antibacterial effect existed between Ag and CeO2. On the one hand, ICP-MS results showed that the existence of CeO2 suppressed the silver ion release rate and provided the composite with the ability of antibrowning; on the other, EPR data indicated that more hydroxyl radicals (·OH) were generated by the interfacial interaction between nanosized Ag and nanosized CeO2. Hence, for the Ag-CeO2 composite antibacterial agent, hydroxyl radicals played an important role in causing bacterial death.

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Faecal contamination and prevalence of pathogenic E. coli in shellfish growing areas along south-west coast of India

Cited by 5 publications

References 48 publications

Prevalence, Antimicrobial Resistance, and Molecular Characterization of Escherichia coli Isolated from Food Contact Surfaces in Seafood Pre-Processing Plants (India)

Prevalence, Antimicrobial Resistance, and Molecular Characterization of Escherichia coli Isolated from Food Contact Surfaces in Seafood Pre-Processing Plants (India)

The Prevalence of Bacteria Commonly Related to the Production of Mussels and Oysters in Saldanha Bay

Preparation and Characterization of an Antibrowning Nanosized Ag-CeO2 Composite with Synergistic Antibacterial Ability

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