Faecal Escherichia coli isolates from healthy dogs harbour CTX-M-15 and CMY-2 β-lactamases

Rocha-Gracia, Rosa del Carmen; Cortés-Cortés, Gerardo; Lozano-Zaraín, Patricia; Bello, Fabio Dal; Martínez‐Laguna, Ygnacio; Torres, Carmén

doi:10.1016/j.tvjl.2014.12.026

Cited by 55 publications

(53 citation statements)

References 47 publications

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“…Fifteen of 20 ESBL-producing isolates, carried PMQR genes with a higher frequency than previously reported [7,8] and in one transconjugant, qnrS1 gene was co-transferred with the bla CTX-M-1 gene. These findings underscore previous results reported in literature stating that use of one of the two antibiotic classes allows a co-selection process [7,8,21]. In Algeria, PMQR genes were reported from clinical human isolates both in hospital and community and were frequently associated with bla CTX-M genes [30,35].…”

Section: Discussionsupporting

confidence: 86%

See 1 more Smart Citation

Extended spectrum β-lactamase and plasmid mediated quinolone resistance in Escherichia coli fecal isolates from healthy companion animals in Algeria

Yousfi

Mairi

Touati

et al. 2016

Journal of Infection and Chemotherapy

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Section: Discussionsupporting

confidence: 86%

“…Our results are relatively higher than in some previous European studies. [16,17], but lower than those previously reported in country beyond Europe like China [20], Tunisia [8], and Mexico [21].…”

Section: Discussioncontrasting

confidence: 58%

Extended spectrum β-lactamase and plasmid mediated quinolone resistance in Escherichia coli fecal isolates from healthy companion animals in Algeria

Yousfi

Mairi

Touati

et al. 2016

Journal of Infection and Chemotherapy

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“…The 40.7% E. coli isolation prevalence in this study is higher than 17, 3.2, 24.5, 20, 1.9 and 3% ESBL-resistant/producing E. coli isolation prevalence reported in faecal samples of 53 healthy dogs in Mexico (Rocha-Gracia et al, 2015), oral swabs of 31 healthy dogs in Brazil (Oliveira et al, 2016), faecal samples of 109 healthy dogs in China (Sun et al, 2010), faecal/urine samples of companion animals in Chile (Moreno et al, 2008), 209 dog faecal deposits in Denmark (Damborg et al, 2015) and among 944 clinical E. coli isolates from companion animals in America (Shaheen et al, 2011), respectively. It is also higher than 16, 7, 6.6 and 2.6% ESBL-resistant/producing E. coli isolation prevalence in 100 extra-clinical faecal samples of dogs in Germany (Schaufler et al, 2015), among 445 clinical E. coli isolates from companion animals in United Kingdom (UK) (Timofte et al, 2016), faecal samples of 75 healthy dogs/cats (Costa et al, 2004) and 39 healthy dogs (Costa et al, 2008) reported in Portugal, respectively.…”

Section: Discussioncontrasting

confidence: 72%

“…Resistance to extended-spectrum β-lactams in enterobacteria is mediated mainly by extended-spectrum β-lactamases (ESBLs) (Tamang et al, 2012;Ljungquist et al, 2016). ESBL-resistant enterobacteria are multidrug-resistant and exhibit resistance to all β-lactams and many classes of antibacterials including 2015a, Cozma et al, 2015b;Baede et al, 2015;Ljungquist et al, 2016), South America (Moreno et al, 2008;Rocha-Gracia et al, 2015;Oleivera et al, 2016;Carvalho et al, 2016), Asia (Sun et al, 2010;So et al, 2012;Tamang et al, 2014) and Australia (Sidjabat et al, 2007). No single report regarding the presence of ESBL-resistant enterobacteria in companion animals in Nigeria, exist in the literature whereas most Nigerian households keep dogs as pets, guard and/or hunting dogs.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Dogs

Anyanwu¹,

Ugwu²,

EZEKWELU³

et al. 2017

Not Sci Biol

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This study was conducted to isolate generic extended-spectrum β-lactam-resistant enterobacteria from household dogs in Nigeria, and to determine the antibacterial resistance profile of the isolates. Rectal swabs were collected from 100, randomly selected, apparently healthy household dogs. Isolation of ESBL-resistant enterobacteria was done using Mac Conkey agar supplemented with 4 µg/ml of ceftazidime. Phenotypic characterization of the isolates to generic level was done following standard biochemical methods. Phenotypic resistance of the isolates to antibacterial agents was determined using the disc diffusion method. Out of 27 ESBL-resistant enterobacterial isolates, 40.7% were Escherichia coli, 37% were Klebsiella species, 18.5% were Salmonella species, while 3.7% was Proteus species. Resistance of the E. coli isolates was 81.8% to ampicillin, 27.2% to streptomycin, 54.5% to ciprofloxacin and tetracycline, 45.4% to enrofloxacin, 90.9% to sulphamethoxazole/trimethoprim, 9.1% to amoxicillin/clavulanic acid and 0% resistant to gentamicin. Resistance of the Klebsiella isolates was 80% to ampicillin, 20% to streptomycin and amoxicillin/clavulanic acid, 30% to ciprofloxacin and enrofloxacin, 60% to tetracycline, 90% to sulphamethoxazole/trimethoprim and 10% to gentamicin. Resistance of the Salmonella isolates was 100% to sulphamethoxazole/trimethoprim, 80% to gentamicin and ampicillin, 60% to streptomycin and tetracycline, 20% to amoxicillin/clavulanic acid, and 0% to ciprofloxacin and enrofloxacin. The Proteus isolate was resistant to streptomycin and gentamycin. All the isolates were resistant to ceftazidime and cefotaxime. Resistance of the isolates to more than 3 classes of antibacterial agents tested was 81.8% for E. coli, 70% for Klebsiella and 100% for Salmonella, respectively. This study has shown that household dogs in Nigeria, are colonized by ESBL-resistant Enterobacteriaceae and are potential reservoirs and disseminators of these organisms.

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“…Few studies, on both healthy and diagnostic clinical canine and feline samples, report finding other ESBL variants including bla SHV-3 in the USA (Shaheen et al, 2011), bla SHV-2 in Mexico (Rocha-Gracia et al, 2015), bla SHV-12 in Italy and Poland (Carattoli et al, 2005b; Rzewuska et al, 2015) and bla SHV-12 in association with bla OXA-48 , bla CMY-2 , bla TEM-1 , aac(6 ′ )-Ib-cr , and qnrB2 in Germany (Stolle et al, 2013). …”

Section: Expansion Toward New Ecological Nichesmentioning

confidence: 99%

A Review of SHV Extended-Spectrum β-Lactamases: Neglected Yet Ubiquitous

2016

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β-lactamases are the primary cause of resistance to β-lactams among members of the family Enterobacteriaceae. SHV enzymes have emerged in Enterobacteriaceae causing infections in health care in the last decades of the Twentieth century, and they are now observed in isolates in different epidemiological settings both in human, animal and the environment. Likely originated from a chromosomal penicillinase of Klebsiella pneumoniae, SHV β-lactamases currently encompass a large number of allelic variants including extended-spectrum β-lactamases (ESBL), non-ESBL and several not classified variants. SHV enzymes have evolved from a narrow- to an extended-spectrum of hydrolyzing activity, including monobactams and carbapenems, as a result of amino acid changes that altered the configuration around the active site of the β -lactamases. SHV-ESBLs are usually encoded by self-transmissible plasmids that frequently carry resistance genes to other drug classes and have become widespread throughout the world in several Enterobacteriaceae, emphasizing their clinical significance.

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Faecal Escherichia coli isolates from healthy dogs harbour CTX-M-15 and CMY-2 β-lactamases

Cited by 55 publications

References 47 publications

Extended spectrum β-lactamase and plasmid mediated quinolone resistance in Escherichia coli fecal isolates from healthy companion animals in Algeria

Extended spectrum β-lactamase and plasmid mediated quinolone resistance in Escherichia coli fecal isolates from healthy companion animals in Algeria

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Dogs

A Review of SHV Extended-Spectrum β-Lactamases: Neglected Yet Ubiquitous

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