Faecal microbiota transplantation‐mediated jejunal microbiota changes halt high‐fat diet‐induced obesity in mice via retarding intestinal fat absorption

Abstract

Summary Faecal Microbiota Transplantation (FMT) is considered as a promising technology to fight against obesity. Wild boar has leanermuscle and less fat in comparison to the domestic pig, which were thought to be related with microbiota. To investigate the function and mechanism of the wild boar microbiota on obesity, we first analysed the wild boar microbiota composition via 16S rDNA sequencing, which showed that Firmicutes and Proteobacteria were the dominant bacteria. Then, we established a high‐fat diet (HFD)‐induced obesity model, and transfer low and high concentrations of wild boar faecal suspension in mice for 9 weeks. The results showed that FMT prevented HFD‐induced obesity and lipid metabolism disorders, and altered the jejunal microbiota composition especially increasing the abundance of the Lactobacillus and Romboutsia, which were negatively correlated with obesity‐related indicators. Moreover, we found that the anti‐obesity effect of wild boar faecal suspension was associated with jejunal N6‐methyladenosine (m6A) levels. Overall, these results suggest that FMT has a mitigating effect on HFD‐induced obesity, which may be due to the impressive effects of FMT on the microbial composition and structure of the jejunum. These changes further alter intestinal lipid metabolism and m6A levels to achieve resistance to obesity.

Gut microbiota response to sucralose or rebaudioside A in rats under two dietary conditions

“…Lactobacillus was higher with sucralose and lower with reb A in HFD. Both genera have been reported to be involved in regulating lipid metabolism, maintaining intestinal epithelial barrier function, and improving diet-induced obesity [31]. Previous studies found that sucralose reduced the bene cial bacteria Lactobacillaceae in healthy [3] or obese rodents [20].…”

Pediococcus acidilactici FZU106 alleviates high-fat diet-induced lipid metabolism disorder in association with the modulation of intestinal microbiota in hyperlipidemic rats

“…In addition, previous study showed that intestinal flora is closely associated with lipid absorption in the intestine ( Martinez-Guryn et al, 2018 ). Recent study by Zhu et al (2021) revealed that fecal microbiota transplantation (FMT) regulates lipid absorption in the intestinal tract by altering the intestinal microbiota composition, which greatly affects the relative abundance of Lactobacillus and Romboutsia in the intestine. It is reported that Lactobacillus and Romboutsia are involved in maintaining intestinal epithelial barrier function, and their metabolites regulate lipid metabolism, thereby improving HFD-induced hyperlipidemia ( Li et al, 2019 ; Russell et al, 2019 ; Yang et al, 2020 ).…”

Effects of Zearalenone on Production Performance, Egg Quality, Ovarian Function and Gut Microbiota of Laying Hens

“…The collected fresh cecum contents were instantly snap frozen in liquid nitrogen and stored at −80 °C [ 58 ] until used for enteric bacteria DNA extraction with the QIAamp DNA stool Mini Kit (Qiagen, Hilden, Germany). The V3-V4 region (338F: ACTCCTACGGGAGGCAGCA, 806R: GGACTACHVGGGTWTCTAAT) of the 16S rDNA was amplified by PCR, and the amplified products were examined by 2% gel electrophoresis.…”