Faecalibacterium prausnitzii Strain HTF-F and Its Extracellular Polymeric Matrix Attenuate Clinical Parameters in DSS-Induced Colitis

Abstract

A decrease in the abundance and biodiversity of intestinal bacteria within the Firmicutes phylum has been associated with inflammatory bowel disease (IBD). In particular, the anti-inflammatory bacterium Faecalibacterium prausnitzii, member of the Firmicutes phylum and one of the most abundant species in healthy human colon, is underrepresented in the microbiota of IBD patients. The aim of this study was to investigate the immunomodulatory properties of F. prausnitzii strain A2-165, the biofilm forming strain HTF-F and the extracellular polymeric matrix (EPM) isolated from strain HTF-F. For this purpose, the immunomodulatory properties of the F. prausnitzii strains and the EPM were studied in vitro using human monocyte-derived dendritic cells. Then, the capacity of the F. prausnitzii strains and the EPM of HTF-F to suppress inflammation was assessed in vivo in the mouse dextran sodium sulphate (DSS) colitis model. The F. prausnitzii strains and the EPM had anti-inflammatory effects on the clinical parameters measured in the DSS model but with different efficacy. The immunomodulatory effects of the EPM were mediated through the TLR2-dependent modulation of IL-12 and IL-10 cytokine production in antigen presenting cells, suggesting that it contributes to the anti-inflammatory potency of F. prausnitzii HTF-F. The results show that F. prausnitzii HTF-F and its EPM may have a therapeutic use in IBD.

Ruthenibacterium lactatiformans gen. nov., sp. nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces

“…Similar elements have been reported in the cell envelope of Gemmiger formicilis (Gossling & Moore, 1975;Salanitro et al, 1976), a member of the family Ruminococcaceae moderately related to strains 585-1 T and 668 based on 16S rRNA gene sequencing. Other related members of the family, A. agile and F. prausnitzii, were shown, however, to have a typical Gram-positive cell-wall architecture with thin murein layer, despite their variable Gram staining (Zellner et al, 1996;Rossi et al, 2015). In addition, a thin microcapsule was visible on the surface of strain 585-1 T .…”

“…Depletion of this organism from the faecal microbiota has been implicated in the pathogenesis of inflammatory bowel disease (IBD; Sokol et al, 2009). Moreover, recent studies have confirmed antiinflammatory activity of F. prausnitzii live cultures, cell supernatants and certain purified components in animal models of IBD (Qu evrain et al, 2015;Rossi et al, 2015). Interestingly, despite its extreme air-sensitivity, F. prausnitzii may actually benefit from low oxygen concentrations by using it for NADH regeneration through an extracellular electron shuttle (Khan et al, 2012).…”

The Surface-Associated Exopolysaccharide of Bifidobacterium longum 35624 Plays an Essential Role in Dampening Host Proinflammatory Responses and Repressing Local T H 17 Responses

“…Similarly, protection against C. rodentium infection by Bifidobacterium breve UCC2003 was dependent on the presence of its exopolysaccharide (21). Furthermore, it was described that an extracellular polymeric matrix, isolated from Faecalibacterium prausnitzii, displayed anti-inflammatory activity in the mouse dextran sodium sulfate colitis model (22).…”

Metabolic characterization and viable delivery of Akkermansia muciniphila for its future application

“…In addition, alterations and interventions in the gut ecosystem may cause diseases, as was discovered in the Nobel Prize winning case of Helicobacter pylori, which can cause ulcerative colitis (Marshall andWarren, 1984, Ahmed, 2005). The influence of bacteria on host health can differ between strains, as was shown for the influence of F. prausnitizii in mice (Rossi, et al, 2015, Song, et al, 2016) and the well-described differences between probiotic strains in human (Hill, et al, 2014). So, to study the causal relationships between bacterial species and compositions in general, we need to culture not only all species, but preferably multiple strains per species.…”

“…These cases included studies using specific probiotic strains (Derrien and van Hylckama Vlieg, 2015), including bifidobacteria (Tojo, et al, 2014) and lactobacilli (Wang, et al, 2015). The use of other potential beneficial bacteria in intervention studies is limited to mouse models and include members of the natural microbiota and possible therapeutic microbes, such as Eubacterium hallii , Faecalibacterium prausnitzii (Rossi, et al, 2015),…”