(1) Background: The aim of this study was to investigate the cyclic fatigue behavior of the newly introduced endodontic instrument systems Tia Tornado Blue and Tia Tornado Gold. (2) Methods: The tested rotary instruments were divided according to their type into four groups as follows: Tia Tornado Blue (TTB), Tia Tornado Gold (TTG), Protaper Gold (PTG), and Vortex Blue (VB). The cyclic fatigue resistance of fifteen instruments of each group, totaling 60, was tested. Each instrument was rotated under continuous motion inside an artificial canal at simulated body temperature at the speed recommended by the manufacturer until fracture. The time to fracture was recorded, and the number of cycles to fracture (NCF) was calculated for each instrument. Additionally, the fractured segment length was calculated, and scanning electron microscopic (SEM) images were captured of the fractured surfaces. (3) Results: Statistical analysis revealed that the VB significantly had the highest NCF followed by the PTG, TTG, and TTB (p < 0.05). The lengths of the fractured segments were found to be similar among the tested instrument, which ranged from 4.42 to 4.86 mm (p > 0.05). SEM images exhibited the typical features of cyclic fatigue. (4) Conclusions: The newly introduced instruments, TTG and TTB, exhibited a significantly lower resistance to cyclic fatigue compared to the PTG and VB rotary instruments.