Cracks always occur for the Q345 steel during cooling, which usually lead to Welding failure. Through investigation of the cracks forming position and propagation path, its forming and propagation mechanism for the melted Q345 steel during cooling was studied in detail by using of SEM, EBSD and Nano Indenter equipment in this paper. SEM observation results imply that cracks mainly initiated at martensite phase region close to basal material. Moreover, it also can be observed that the cracks always propagated along the martensite lath or packets boundaries, but not the original prior austenite grain boundaries. The hardness differences between cracks adjacent positions and matrix is about 2.48 GPa, which is larger than that of melted region and matensite (0.70GPa). In addition, EBSD results show that the average prior austenite grains boundaries angles (30-50º) are always smaller than that of martensite lath and packet boundaries (>50º). Combining investigation of the cracks forming position, propagation path and microstructure misorientation, it proves that the occurrence of the cracks at junction of martensite phase region close to basal material is related with larger hardness difference or larger residual stress between martensite and ferrite, while cracks propagation path is controlled by the boundaries angles or local misorientation. The cracks incline to propagate along or deflect to large martensite lath and packets boundaries.