Failure of accumulation of cellular RNA in hamster cells stimulated to synthesize DNA by infection with adenovirus 2.

Pochron, Sharon F.; Rossini, Mara; Darzynkiewicz, Zbigniew; Traganos, Frank; Baserga, Renato

doi:10.1016/s0021-9258(19)85504-7

Cited by 49 publications

(2 citation statements)

References 22 publications

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“…Thus, in ts4F8 celLs, a shift to the nonpermissive temperature inhibits entry into the S phase, but does not inhibit rRNA synthesis (35) or accumulation (3). Adenovirus 2 can stimulate cell DNA synthesis (37) without stimulating rRNA synthesis (42) or accumulation (31). Finally, in the SV40 genome, the sequences that are critical for the induction of cell DNA synthesis are separate from the sequences that are critical for the stimulation of rRNA synthesis (13,41).…”

Section: Resultsmentioning

confidence: 99%

Induction of Cellular Deoxyribonucleic Acid Synthesis in Butyrate-Treated Cells by Simian Virus 40 Deoxyribonucleic Acid

Kawasaki

Diamond

Baserga

1981

Molecular and Cellular Biology

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Sodium butyrate (3 mM) inhibited the entry into the S phase of quiescent 3T3 cells stimulated by serum, but had no effect on the accumulation of cellular ribonucleic acid. Simian virus 40 infection or manual microinjection of cloned fragments from the simian virus 40 A gene caused quiescent 3T3 cells to enter the S phase even in the presence of butyrate. NGI cells, a line of 3T3 cells transformed by simian virus 40, grew vigorously in 3 mM butyrate. Homokaryons were formed between G1 and S-phase 3T3 cells, Butyrate inhibited the induction of deoxyribonucleic acid synthesis that usually occurs in B1 nuclei when G1 cells are fused with S-phase cells. However, when G1 3T3 cells were fused with exponentially growing NGI cells, the 3T3 nuclei were induced to enter deoxyribonucleic acid synthesis. In tsAF8 cells, a ribonucleic acid polymerase II mutant that stops in the G1 phase of the cell cycle, no temporal sequence was demonstrated between the butyrate block and the temperature-sensitive block. These results confirm previous reports that certain virally coded proteins can induce cell deoxyribonucleic acid synthesis in the absence of cellular functions that are required by serum-stimulated cells. Our interpretation of these data is that butyrate inhibited cell growth by inhibiting the expression of genes required for the G0 leads to G1 leads to S transition and that the product of the simian virus 40 A gene overrode this inhibition by providing all of the necessary functions for the entry into the S phase.

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Section: Resultsmentioning

confidence: 99%

Induction of Cellular Deoxyribonucleic Acid Synthesis in Butyrate-Treated Cells by Simian Virus 40 Deoxyribonucleic Acid

Kawasaki

Diamond

Baserga

1981

Molecular and Cellular Biology

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“…Altered control of the cell growth cycle must therefore be a direct effect of ElA, perhaps related to the function of ElA in transformation. ElA appears to induce the S-phase program without many of the earlier Gl events that normally precede it (1,8,9,23). Indirect evidence suggests that expression of c-myc, which is distantly related to ElA (24), is involved in the chain of events that occur when quiescent cells are stimulated to enter a cell cycle (15; A. W. Braithwaite, unpublished data).…”

Section: Discussionmentioning

confidence: 99%

Control Functions of Adenovirus Transformation Region E1A Gene Products in Rat and Human Cells

Bellett

David

et al. 1985

Molecular and Cellular Biology

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Altered control of the rat cell cycle induced by adenovirus requires expression of transformation region E1A, but not of E1B, E2A, E2B, or late genes. We show here that neither E3 nor E4 is required, so the effect results directly from an E1A product. Mutants with defects in the 289-amino-acid (aa) E1A product had little or no effect on the rat cell cycle even at 1,000 IU per cell. A mutant (pm975) lacking the 243-aa E1A product altered cell cycle progression, but less efficiently than did wild-type virus. The 289-aa E1A protein is therefore essential for cell cycle effects; the 243-aa protein is also necessary for the full effect but cannot act alone. Mutants with altered 289-aa E1A proteins showed different extents of leak expression of viral early region E2A as the multiplicity was increased; each leaked more in human than in rat cells. dl312, with no E1A products, failed to produce E2A mRNA or protein at 1,000 IU per cell in rat cells but did so in some experiments in human cells. There appears to be a very strict dependence of viral early gene expression on E1A in rat cells, whereas dependence on E1A is more relaxed in HeLa cells, perhaps due to a cellular E1A-like function. Altered cell cycle control is more dependent on E1A function than is early viral gene expression.

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New cell cycle compartments identified by multiparameter flow cytometry

1980

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Simultaneous measurements of cellular DNA and RNA as well as estimates of the sensitivity of DNA in situ to denaturation by acid (which correlates with the degree of chromatin condensation) and cell ability to incorporate 5‐bromodeoxyuridine (BUdR), performed on over 40 different cell systems enabled us to subclassify cells into 12 functionally distinct cell cycle compartments. Quiescent cells had low RNA values, DNA very sensitive to denaturation, and they did not incorporate BUdR. Although in most cell systems they had 2C DNA content (G1Q), quiescent cells with higher DNA values (SQ and G2Q) were also seen. The G1 phase of exponentially growing cells had two distinct compartments, A and B. G1 cells entered S phase only from the B compartment. An increase in RNA and a decrease in the sensitivity of DNA to acid denaturation beyond a specific level characterized the transition of cells from the A to B compartments. Since the G1A to G1B transition was not linear but exponential, it may be assumed that a non‐deterministic event triggering cell progression into the cycle resides within G1A. Under adverse growth conditions the probability of a G1A to G1B transition decreased. Cells In G2 had DNA more sensitive to acid denaturation than S or G1 phase cells. Mitotic cells had the most condensed chromatin and their RNA content was twice that of G1A cells. Differentiated cells were characterized by 2C DNA (G1D) but, depending on the cell type, had varying RNA content and different degrees of chromatin condensation. A hitherto undescribed category of cells undergoing transition from quiescence to the cycle (or vice versa) was distinguished based on their intermediate values of RNA, sensitivity of DNA to acid denaturation and inability to incorporate BUdR at the rate characteristic for S cells. Depending on their DNA content (C) at the time of transition these cells could be classified as G1T (2C), ST (4>C>2) or G2T (4C).

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Failure of accumulation of cellular RNA in hamster cells stimulated to synthesize DNA by infection with adenovirus 2.

Cited by 49 publications

References 22 publications

Induction of Cellular Deoxyribonucleic Acid Synthesis in Butyrate-Treated Cells by Simian Virus 40 Deoxyribonucleic Acid

Induction of Cellular Deoxyribonucleic Acid Synthesis in Butyrate-Treated Cells by Simian Virus 40 Deoxyribonucleic Acid

Control Functions of Adenovirus Transformation Region E1A Gene Products in Rat and Human Cells

New cell cycle compartments identified by multiparameter flow cytometry

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