Failure of antibiotics gentamycin, tylosin, lincomycin and spectinomycin to eliminate in artificially infected frozen bovine semen

Visser, Imke; Laak, E. A. ter; Jansen, HB

doi:10.1016/s0093-691x(99)00018-7

Cited by 17 publications

(21 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results are in agreement with more recent studies in ruminants. The combination of GTLS decreased the number of bacteria in stored buffalo semen more effectively than streptomycin and penicillin (Akhter et al 2008) while in stored bovine semen, this GTLS concentration was not effective to control mycoplasma growth in all samples (Visser et al 1999).…”

Section: Discussionmentioning

confidence: 86%

See 1 more Smart Citation

Cooled Storage of Canine Semen:in vitroEffects of Different Concentrations of an Antibiotic Combination on Growth of Mollicutes

Becher¹,

Spergser

Aurich³

et al. 2013

Reprod Domestic Animals

View full text Add to dashboard Cite

The aim of this study was to examine effects of an antibiotic combination at different concentrations on growth of mycoplasma and ureaplasma during cooled storage of canine semen (n = 20). Semen aliquots were diluted with Tris-citric acid-fructose-egg yolk extender containing either 1.0 g/l streptomycin and 0.6 g/l benzylpenicillin (control) or a combination of gentamycin, tylosin, lincomycin and spectinomycin (GTLS-1: 0.25, 0.05, 0.15 and 0.3; GTLS-2: 0.5, 0.1, 0.3 and 0.6; GTLS-3: 1.0, 0.2, 0.6 and 1.2 g/l). Samples were assessed for motility and membrane integrity by computer-assisted sperm analysis immediately after dilution and at 24, 48 and 72 h of cooled storage. Morphologically, normal spermatozoa were determined, and bacterial culture was performed at 24 and 72 h. Mycoplasma spp. were detected in 14 of 20 ejaculates (70%) with severe growth in 12 samples. A reduction but not total elimination of mycoplasma growth occurred in all GTLS extenders with the most pronounced reduction in group GTLS-3 (control vs GTLS-1 and GTLS-2 p < 0.05, control vs GTLS-3 p < 0.001). Ureaplasmas were detected in four ejaculates, and growth was reduced to the same extent in GTLS and control extender. Progressive motility in all groups, total motility in groups GTLS 1-3 and percentage of membrane-intact spermatozoa in groups GTLS 2 and 3 decreased slightly (p < 0.05) over time. In conclusion, dilution of canine semen with GTLS extender has no major detrimental effects on spermatozoa during cooled storage. It reduced the growth but did not totally eliminate mycoplasmas and ureaplasmas from cooled-stored dog semen.

show abstract

Section: Discussionmentioning

confidence: 86%

“…) while in stored bovine semen, this GTLS concentration was not effective to control mycoplasma growth in all samples (Visser et al. ).…”

Section: Discussionmentioning

confidence: 99%

Cooled Storage of Canine Semen:in vitroEffects of Different Concentrations of an Antibiotic Combination on Growth of Mollicutes

Becher¹,

Spergser

Aurich³

et al. 2013

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…The presence of a cryoprotectant like glycerol in the extender used for semen dilution (Foote, 1970) enhances the natural ability of MAP to survive in such specific conditions as has been demonstrated for other microorganisms (Bonadonna, 1971;Visser et al, 1999).…”

Section: Probable Number Of Organisms In Semenmentioning

confidence: 93%

Opinion of the Scientific Panel on Animal Health and Welfare (AHAW) on a request from the Commission related to the risk of transmission of Mycobacterium avium subsp. paratuberculosis via bovine semen

2004

EFSA Journal

View full text Add to dashboard Cite

“…The effect of Gentamycin, Tylosin, Lincomycin, Spectinomycin (GTLS) is also discussed in some studies. Only a bacteriostatic effect of GTLS upon Mycoplasma in frozen–thawed (artificially infected) semen is found (Visser et al. 1998).…”

Section: Semen Collection and Processingmentioning

confidence: 99%

Ways to Improve the Biosecurity of Bovine Semen

Ruigh¹,

Bosch²,

Brus³

et al. 2006

Reprod Domestic Animals

View full text Add to dashboard Cite

Semen production and trade is a worldwide industry. A framework, based on international standards is awaiting international and national regulation. In the perspective of biosecurity of the final product, critical notes can be made according to the semen production regulation and product safety. Process description brings the obligatory health standards for the production bulls, collection and processing of semen, identification, registration, worldwide distribution and insemination into discussion. Test frequency, test quality and demands, way of sampling and test consistency can influence product safety. New scientific knowledge can influence the value of the regulation. Whether a country is free of notifiable disease should influence decisions regarding necessary tests for the production bulls. The biosecurity of the semen production process is influenced by several factors. The effectiveness of the antibiotics used is questionable. The extenders for cryopreservation added to the semen can affect product safety. The way materials and storage containers have to be disinfected must be clear. In modern industry, tracking and tracing is an important issue. Worldwide differences in ways of identification of straws do not benefit a proper identification and registration throughout the process. Regulation could help improve the transparency of production and trade. Before anything concerning biohazard is implemented in regulation, each rule should be thoroughly based on scientific research where possible and furthermore it must be possible to enforce the regulation. The effort it takes to enforce the regulation should be in balance with the benefit it provides. An approach to alter regulation quickly is advisable. To produce a safe product that is accepted for international trade is of vital interest for the survival of artificial insemination (AI) in cattle.

show abstract

Failure of antibiotics gentamycin, tylosin, lincomycin and spectinomycin to eliminate in artificially infected frozen bovine semen

Cited by 17 publications

References 8 publications

Cooled Storage of Canine Semen:in vitroEffects of Different Concentrations of an Antibiotic Combination on Growth of Mollicutes

Cooled Storage of Canine Semen:in vitroEffects of Different Concentrations of an Antibiotic Combination on Growth of Mollicutes

Opinion of the Scientific Panel on Animal Health and Welfare (AHAW) on a request from the Commission related to the risk of transmission of Mycobacterium avium subsp. paratuberculosis via bovine semen

Ways to Improve the Biosecurity of Bovine Semen

Contact Info

Product

Resources

About