Fall in implantation rates following ICSI with sperm with high DNA fragmentation

Speyer, B. E.; Pizzey, Arnold; Ranieri, M.; Joshi, Rajiv; Delhanty, Jda; Serhal, Paul

doi:10.1093/humrep/deq116

Cited by 126 publications

(101 citation statements)

References 39 publications

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“…Twenty studies [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] were indentified for our meta-analysis. The flow chart of the process for the identification of the studies were shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…A total of 15 studies with CP as the outcome measurement was included in the meta-analysis [7,8,10,12,[15][16][17][19][20][21][22][23][24][25][26]. These studies were divided into 3 groups according to the DFI cutoff value (>27 %, 15-27 %, ≤15 %) as described previously.…”

Section: Dfi and Cpmentioning

confidence: 99%

“…When the studies with a DFI cutoff value >27 % were pooled into the meta-analysis [8,10,15,17,19,20,22,24,25], the pooled OR demonstrated that the couples were more likely to achieve CP if the DFI was <27 % (OR (95%CI) = 1.437 (1.186-1.742), p=0.000). As for the heterogeneity test, the I 2 value between the studies was 52.5 %, indicated a moderate heterogeneity.…”

Section: Dfi and Cpmentioning

confidence: 99%

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Sperm DNA fragmentation index and pregnancy outcome after IVF or ICSI: a meta-analysis

Zheng

Zhu

Jiang

et al. 2014

J Assist Reprod Genet

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Purpose The purpose of this study was to carry out a metaanalysis for a comprehensive understanding and estimation of the association between sperm DNA Fragmentation Index (DFI) and pregnancy outcome after in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) treatment. Methods Studies concerning the link of DFI with pregnancy outcome were included after literature search of database PUBMED, EMBASE, MEDLINE. Related information was extracted from the eligible studies by two independent authors and a meta-analysis was conducted by using STATA 12.0 software. Pregnancy outcomes consisted of biochemical pregnancy (BP), clinical pregnancy (CP) and pregnancy loss (PL). The studies included for meta-analysis were divided into three groups according to the DFI threshold value (DFI >27 %, 15-27 %, ≤15 % group). The odds ratio (OR ) and their 95 % confidence intervals (95 % CIs) were used to evaluate the association between DFI and pregnancy outcome. Results Twenty articles were included in our meta-analysis. The results indicated that infertile couples were more likely to get pregnant if DFI was less than threshold value (For threshold value > 27 % and 15-27 % group, combined overall OR (95 % CI) = 1.437 (1.186-1.742), 1.639 (1.093-2.459) respectively). However, when stratified by DFI detection methods, using sperm chromatin structure assay (SCSA) as the DFI test method, the results indicated a similar CP rate between groups with a high DFI or a lower DFI value (SCSA, For threshold value >27 % and 15-27 % group, combined overall OR (95 % CI) = 1.242(0.978-1.577), 1.480(0.921-2.377) respectively). The meta-analysis based on BP (overall OR (95 % CI) = 0.952 (0.697-1.302)) and PL((For DFI >27 %, 15-27 %, ≤15 % group, OR (95 % CI) = 0.786 (0.491-1.258), 1.509 (0.655-3.476), (0.538 (0.264-1.097) respectively) outcome yielded nonsignificant results. Conclusions The predication value of DFI for IVF or ICSI outcome is not confirmed in our meta-analysis. Further better designed studies with larger subjects involved are needed to better address this issue.

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Section: Resultsmentioning

confidence: 99%

Section: Dfi and Cpmentioning

confidence: 99%

Section: Dfi and Cpmentioning

confidence: 99%

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Sperm DNA fragmentation index and pregnancy outcome after IVF or ICSI: a meta-analysis

Zheng

Zhu

Jiang

et al. 2014

J Assist Reprod Genet

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“…Fertilization with DNA-damaged sperm increases the risk of promoting pregnancy loss in mammals (Speyer et al 2010), arrest in embryo development or embryo death (Perez-Cerezales et al 2010b, Gawecka et al 2013, birth defects, chromosomal abnormalities and other genetic diseases (Fernandez-Gonzalez et al 2008, Barroso et al 2009, Schulte et al 2010, Marchetti et al 2015. The DNA-repairing activity relies on the oocyte machinery once the fertilization takes place (Aitken et al 2014, Fernandez-Diez et al 2015, the zygote being responsible for repairing the sperm damage.…”

Section: Introductionmentioning

confidence: 99%

Impact of sperm DNA damage and oocyte-repairing capacity on trout development

González-Rojo¹,

Lombó²,

Herráez³

2016

Reproduction

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“…4,5 It is possible that some ICSI patients with low implantation or pregnancy may result from injection of inadequate or poor-quality sperm, for example, with abnormal morphological or DNA damaged sperm. [6][7][8][9] As the majority of ICSI patients are male factor infertility and they have variable abnormal ejaculated sperm, particularly those with high proportion of DNA damaged sperm, subjective manual method is inconsistent or unable to select a most competent sperm for ICSI. Thus it is necessary to develop an objective and efficient method for selection of the most competent sperm for ICSI.…”

mentioning

confidence: 99%

Could using the zona pellucida bound sperm for intracytoplasmic sperm injection (ICSI) enhance the outcome of ICSI?

Liu¹

2011

Asian J Androl

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I n the recent literature, several interesting articles have been published using the zona pellucdia (ZP)-bound sperm for intracytoplasmic sperm injection (ICSI) to enhance embryo quality, implantation and clinical pregnancy rates. [1][2][3] The three studies were performed independently in different ART clinics. In the test group, patients' sibling immature (either germinal vesicle (GV) or metaphase I) oocytes were used to harvest the ZP-bound sperm, which were used for injection of mature oocytes. In the control group, ICSI patients with similar female age and sperm profiles had ICSI with standard sperm selection by the embryologist subjectively choosing the sperm. 2,3 The results showed that the proportion of high-quality embryos (grades 1 and 2) and implantation rates were higher with ICSI using ZP-bound sperm than with conventional sperm selection. More pregnancies were achieved in the ZP-bound sperm group although clinical (fetal heart detected) pregnancy rates did not reach statistical significance between the two groups, likely due to small sample sizes. These preliminary reports suggest that ZPselected sperm appear to be superior to subjectively selected sperm for ICSI. If this finding can be further verified by a large clinical study, the use of the ZP-bound sperm for ICSI could be applied more widely in ICSI to enhance pregnancy outcome.In the literature, some studies have been reported that in vitro fertilization (IVF) embryos produced relatively higher implantation and clinical pregnancy rates compared to ICSI embryos. 4,5 It is possible that some ICSI patients with low implantation or pregnancy may result from injection of inadequate or poor-quality sperm, for example, with abnormal morphological or DNA damaged sperm. [6][7][8][9] As the majority of ICSI patients are male factor infertility and they have variable abnormal ejaculated sperm, particularly those with high proportion of DNA damaged sperm, subjective manual method is inconsistent or unable to select a most competent sperm for ICSI. Thus it is necessary to develop an objective and efficient method for selection of the most competent sperm for ICSI.Over the many years since ICSI was first introduced in 1992, ICSI has been using the same sperm preparation methods as conventional IVF, such as swim-up or discontinuous density gradients centrifugation. These classic methods are predominately designed to harvest large numbers of motile sperm and to eliminate non-sperm cells and debris. However, these sperm preparation methods are less or not effective in the selection of sperm with normal morphology and normal DNA. [10][11][12][13] For conventional IVF, it is adequate to inseminate oocytes using motile sperm selected by these methods since the sperm which fertilizes will be further selected through the biological process of spermoocyte interaction, especially sperm-ZP binding. [14][15][16][17] In contrast with conventional ICSI, this additional selection does not occur. The embryologists subjectively select a sperm based only on motility and gr...

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Fall in implantation rates following ICSI with sperm with high DNA fragmentation

Abstract: These observations may help to resolve the issues about how, and to what extent, sperm DNA damage impacts upon the success of IVF and ICSI procedures.

Cited by 126 publications

References 39 publications

Sperm DNA fragmentation index and pregnancy outcome after IVF or ICSI: a meta-analysis

Sperm DNA fragmentation index and pregnancy outcome after IVF or ICSI: a meta-analysis

Impact of sperm DNA damage and oocyte-repairing capacity on trout development

Could using the zona pellucida bound sperm for intracytoplasmic sperm injection (ICSI) enhance the outcome of ICSI?

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