Fall in the number of myenteric neurons in aging guinea pigs

Gabella, Giorgio

doi:10.1016/0016-5085(89)90516-7

Cited by 174 publications

(164 citation statements)

References 6 publications

Supporting

Mentioning

121

Contrasting

Unclassified

Order By: Relevance

“…The explanation is based on the difference of age between the rats: while the animals of this study had seven months of age, those had three and five months on the sacrifice. This decrease on the neuronal population is also described by other authors on the intestine of rats 15 and guinea-pigs 24 , and on the small and large intestines of humans 25,26 , and is due to aging by itself, independently of pathophysiological conditions.…”

Section: Discussion Discussion Discussion Discussion Discussionsupporting

confidence: 82%

Number and size of myenteric neurons of the duodenum of adult rats with acute diabetes

Furlan

Miranda-Neto

Sant’Ana

et al. 1999

Arq. Neuro-Psiquiatr.

View full text Add to dashboard Cite

-This study had as its purpose to assess the effects of acute diabetes induced by streptozotocin (35 mg/kg body weight) on the number and size of the myenteric neurons of the duodenum of adult rats considering equally the antimesenteric and intermediate regions of the intestinal circumference. Experimental period extended for a week. Neuronal counts were carried out on the same number of fields of both regions of the duodenal circumference and measurements of neuronal and nuclear areas on equal numbers of cells. Number and size of the myenteric neurons stained with Giemsa were not significantly different between groups. On the other hand, the proportion of NADH-positive neurons increased from 18.54% on the controls to 39.33% on the diabetics. The authors discuss that this increased reactivity probably results from a greater NADH/NAD + ratio, described in many tissues of diabetic animals, which has consequences on the modulation of the enzymes that use these cofactors and whose activity is detected by the NADH-diaphorase technique.KEY WORDS: duodenum, myenteric neurons, acute diabetes, NADH-diaphorase.Número e tamanho dos neurônios mientéricos do duodeno de ratos adultos com diabetes agudo RESUMO -Este estudo teve como objetivo avaliar os efeitos do diabetes agudo induzido por estreptozootocina (35 mg/kg de peso corporal) sobre o número e tamanho dos neurônios mientéricos do duodeno de ratos adultos considerando de forma equivalente as regiões antimesentérica e intermediária da circunferência intestinal. O período experimental se estendeu por uma semana. As contagens neuronais foram feitas em igual número de campos nas duas regiões da circunferência duodenal e as mensurações das áreas neuronais e nucleares em igual número de células. O número e o tamanho dos neurônios corados por Giemsa não foram significativamente diferentes entre os grupos. Por outro lado, a proporção de neurônios NADH-positivos aumentou de 18,54% nos animais controles para 39,33% nos diabéticos. Os autores discutem que essa maior reatividade possivelmente resultou do aumento da proporção NADH/NAD + , descrita em diversos tecidos de animais diabéticos, que repercute na modulação das enzimas que utilizam esses cofatores e cuja atividade é detectada pela técnica da NADHdiaforase. PALAVRAS-CHAVE: duodeno, neurônios mientéricos, diabetes agudo, NADH-diaforaseThe knowledge about the mammalian enteric nervous system (ENS) can be considered, currently, as wide and in some instances, quite deep. Descriptions found on the literature assess several aspects of this subdivision of the autonomic nervous system 1-5 and reveal the richness of structure and organization of this neuronal population of magnitude similar to that of the spinal Trabalho

show abstract

Section: Discussion Discussion Discussion Discussion Discussionsupporting

confidence: 82%

Number and size of myenteric neurons of the duodenum of adult rats with acute diabetes

Furlan

Miranda-Neto

Sant’Ana

et al. 1999

Arq. Neuro-Psiquiatr.

View full text Add to dashboard Cite

show abstract

“…However, number of myenteric neurons of PM 1.5 group was significantly larger than that of other groups, and number of myenteric neurons of PM 24 group significantly decreased (17%) compared to PM 3, PM 6 and PM 12 groups in the gerbil duodenum. This result is similar to the previous studies in the guinea pig (-18%), human (34%), mouse (-40%) and rat (15-25%) [12,13,19,36,40,41].…”

Section: Discussionsupporting

confidence: 92%

Age-Related Changes of Calbindin D-28k-Immunoreactive Neurons in the Myenteric Plexus of Gerbil Duodenum

Choi

Lee

Chung

et al. 2008

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. We examined the age-related changes of calbindin D-28k (CB)-immunoreactive neurons and overall populations of neurons in the myenteric plexus of gerbil duodenum using whole mount preparations and immunohistochemistry. The circumference of duodenum increased age-dependently. CB-immunoreactive neurons were observed in all groups, and most of them had the Dogiel type II morphology. The fully developed cobweb-like structures were observed in the myenteric plexus of duodenum at postnatal month (PM) 3 to 24. Although the highest numbers of CB-immunoreactive neurons and overall population were observed in PM 1.5, it is related with significant increase of the size of circumference between PM 1.5 to PM 3. CB-immunoreactive neurons were slightly decreased with age between PM 3 to PM 24. We have also found that whole numbers of myenteric neurons were also significantly decreased in PM 24 group. These results suggest that loss of overall numbers of myenteric neurons and CB-immunoreactive neurons may be related with age-related neurodegeneration and functional loss of duodenum in the gerbil. KEY WORDS: aging, calbindin, duodenum, gerbil, myenteric plexus.J. Vet. Med. Sci. 70(4): 343-348, 2008 The autonomic nervous system is divided into two divisions, e.g. parasympathetic nervous system and sympathetic nervous system. In recent decade, the enteric nervous system (ENS) is the largest division of the autonomic nervous system, because it can perform its function independently from the central nervous system [7,20,44]. It consists of local nerve networks embedded in the gut wall and is subdivided into two ganglionated plexuses, i.e., the submucosal and myenteric plexuses [40,44]. The ENS mediates complex reflex activities involving intestinal motility, mucosal transportation and blood flow [7]. In electrophysiological studies, the majority of the enteric neurons could be placed in one of two classes: synaptic neurons or after-hyperpolarizing (AH) neurons [21,22,26,29,34].The myenteric plexus lies between the longitudinal and circular muscular layers and extends entire length of the gut. It has been known that the myenteric plexus primarily provides motor innervation to the two muscular layers and secremotor innervation to the mucosa [15,44].Calbindin D-28k (CB), one of the calcium binding proteins, plays an important role as an intracellular facilitator of calcium diffusion or as a modulator of neurotransmission [3,4,16,17]. In neurons, action potentials are also carried in part by inward calcium current [22,26,34]. It has been reported that AH neurons were sensory neurons because these neurons lacked fast excitatory synaptic inputs [21,22,26,29,34]. More recently, with the help of the combination of the electrophysiological and immunohistochemical methods, it has been identified that the AH neurons had Dogiel type II morphology [14,18,26].Although the distribution of CB-containing neuronal system has been reported upon the forebrain [11,23,24], comprehensive knowledge about the maturation of CB neurons in myenteri...

show abstract

“…18,19 Similar findings have been demonstrated in aging mice, rats, guinea pigs, and a nonhuman primate model. [20][21][22][23] The extent of age-related loss of enteric neurons is controversial to date. In humans and rats, selective loss of cholinergic neurons with sparing of nitrergic neurons has been reported.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Changes in the Interstitial Cells of Cajal and Neuronal Nitric Oxide Synthase-positive Neuronal Cells With Aging Between the Ascending and Descending Colon of F344 Rats

Lee

Kim

et al. 2017

J Neurogastroenterol Motil

View full text Add to dashboard Cite

Background/AimsNeuronal degeneration and changes in interstitial cells of Cajal (ICCs) are important mechanisms of age-related constipation. This study aims to compare the distribution of ICCs and neuronal nitric oxide synthase (nNOS) with regard to age-related changes between the ascending colon (AC) and descending colon (DC) in 6-, 31-, and 74-week old and 2-year old male Fischer-344 rats. MethodsThe amount of fecal pellet and the bead expulsion times were measured. Fat proportion in the muscle layer of the colon was analyzed by hematoxylin and eosin staining. Proto-oncogene receptor tyrosine kinase (KIT) and neuronal nitric oxide synthase (nNOS) expression were analyzed with Western blotting and immunohistochemistry. Isovolumetric contractile measurements and electrical field stimulation were used to assess smooth muscle contractility. ResultsColon transit and bead expulsion slowed with senescence. Fat in the muscle layer accumulated with age in the AC, but not in the DC. The proportion of KIT-immunoreactive ICCs in the submucosal and myenteric plexus was higher in the DC than in the AC, and it declined with age, especially in the AC. In contrast, the proportion of NOS-immunoreactive neurons in the myenteric plexus was higher in the AC than in the DC, and both decreased in older rats. Nitric oxide levels declined with age in the DC. Muscle strip experiments showed that the inhibitory response mediated by nitric oxide in the circular direction of the DC was reduced in 2-year old rats. ConclusionThe AC and DC differ in their distribution of ICCs and nNOS, and age-related loss of nitrergic neurons more severely affects the DC than the AC.

show abstract

Fall in the number of myenteric neurons in aging guinea pigs

Cited by 174 publications

References 6 publications

Number and size of myenteric neurons of the duodenum of adult rats with acute diabetes

Number and size of myenteric neurons of the duodenum of adult rats with acute diabetes

Age-Related Changes of Calbindin D-28k-Immunoreactive Neurons in the Myenteric Plexus of Gerbil Duodenum

Comparison of Changes in the Interstitial Cells of Cajal and Neuronal Nitric Oxide Synthase-positive Neuronal Cells With Aging Between the Ascending and Descending Colon of F344 Rats

Contact Info

Product

Resources

About