False-Negative Culture Results with Fungal Isolates from Peritoneal Dialysis Fluid

Pimentel, Jason D.; Lum, Gary

doi:10.1128/jcm.44.3.1206.2006

Cited by 1 publication

(1 citation statement)

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“…Mycology culture is still considered the gold standard despite of being an old traditional diagnostic technique. Culture practice should not be ignored, even with delay in fungal identification [10,11]. Non-molecular markers are available alternative tools supporting the diagnosis of fungal infection in combination with clinical, radiologic, and other microbiological criteria [12].…”

Section: Introductionmentioning

confidence: 99%

Laboratory approach for detection of Non-invasive fungal Rhinosinusitis: A case-control study

ELBadawy¹,

Meawed

El‐Anwar³

2016

int. arab. j antimicrob. agents

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Objective: Noninvasive fungal rhinosinustis (NFRS) is a problematic clinical condition due to its resistant to traditional medical treatment. Surgical debridement is the basis for its treatment. Mycology laboratory investigation can help to solve this issue. This case control study was designed to investigate the frequency of NFRS in our locality and identify fungal species incriminated in this clinical condition at the Otorhinolaryngology department, Zagazig University Hospitals. The study evaluated the role of microscopic examination, antigen detection and PCR in comparison to culture methods used in diagnosis of fungal agents. Materials: Sinus materials were collected from 78 cases which have been clinically and radiologically confirmed with NFRS over the period June 2013 to September 2015. A control group 78 subjects were included with healthy sinuses from whom nasal smears were obtained. Samples were processed in the Mycology Laboratory, and all specimens were examined microscopically in 10% KOH preparations. Lactophenol cotton blue slide preparations were used to detect microscopic structures of hyphae and conidia. PCR amplification of the extracted DNA was performed using fungal universal primers for amplification of 28 S rDNA genes. Results: Microscopic examinations revealed hyphae and fruiting bodies in 37 (47.4%) of the cases. Fungal culture detected 36 patients infected with NFRS. Aspergillus fumigatus was the most frequently isolate from fungal rhinosinusitis (52.7 %) of the cases, followed by Penicillium spp. (22.2%). PCR amplification exhibits the same sensitivity and specificity as those demonstrated by microscopic examination (100% and 97.3%,

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Section: Introductionmentioning

confidence: 99%