False paternity with one or two mismatches using commercial STR kits

Akhteruzzaman, Sharif; Majumder, Ashish Kumar; Ferdous, Ahmad; Ali, Mohammad Eunus

doi:10.1080/00450618.2011.650209

Cited by 8 publications

(7 citation statements)

References 18 publications

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“…The result of amplification of the 22 autosomal STRs along with Amelogenin for the trio is presented (Table 1). Paternal and maternal mismatch at various STR loci has been reported by several workers [8], [9], [10], [11]. Genotype at the locus D13S317 in father, deceased and mother was 10/11, 11/11 and 10/10 respectively (Table 3).…”

Section: Resultsmentioning

confidence: 64%

Maternal allele mutation: Slippage synthesis furnishing evolutionary trend

Kumar¹,

Kumar²,

Kumawat³

et al. 2021

GSC Biol. and Pharm. Sci.

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Gain or loss of repeat motifs leads to an allelic mismatch in the disputed child which is a deviation from the Mendelian inheritance, thereby leading to a paternal mismatch of putative father or exclusion of mother in case of maternal allelic mismatch. This allelic mismatch at one or more loci is a major cause of forensic inferences. The biological samples of the case were genotyped using Powerplex®- Fusion 5C system kit and Investigator® Argus X-12 QS kit as per recommendations of the manufacturer. In this case, identification of a putrefied dead body with 22 autosomal STR loci, primarily analyzed by Powerplex®- Fusion 5C system kit divulged a maternal mismatch at locus D13S317. Alleles at the locus D13S317 allegedly belonging to the father of the deceased and the mother were observed as 10/11, 11/11 and 10/10 respectively. To rule out allelic mismatch at this particular locus, 12 X-STR loci were amplified, in which all the maternal alleles of deceased completely matched with the mother. This case study indicates the extension of one microsatellite repeat motif (TATC) at locus D13S317 in the population of Rajasthan. The reported mutation rate was 0.14% and 0.04% at locus D13S317 in paternal and maternal meiosis respectively.

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Section: Resultsmentioning

confidence: 64%

Maternal allele mutation: Slippage synthesis furnishing evolutionary trend

Kumar¹,

Kumar²,

Kumawat³

et al. 2021

GSC Biol. and Pharm. Sci.

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“…Two non-matched CODIS STRs were found at TPOX and D13S317 loci, seemingly ruling out AF2 and AF3, respectively, as the real father. However, previous researchers [4][5][6]12] suggest that exclusion of paternity should be based on exclusionary events at two or more loci, meaning that the single CODIS STR, TPOX (AF2) and D13S317 (AF3), would be insufficient for a judgment. Comparatively, from the non-CODIS STRs, 7 (AF2) and 11 (AF3) exclusionary loci were identified.…”

Section: Resultsmentioning

confidence: 99%

“…Because of the effects of paternal mutations, a minimum of two exclusions of independent loci would generally be required for a regular paternity testing to reach a conclusion that an alleged father is not the real father of a disputed child. Since a wrong paternity exclusion case with two mismatches was reported [4], in which the putative father is indeed the biological father.…”

mentioning

confidence: 99%

Non-CODIS DNA markers could be more effective than CODISbased STRs in problematic biological relationship cases

Tsai¹,

Yang²,

Chou³

et al. 2013

RJLM

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Short tandem repeat (STR) markers are used worldwide for forensic and paternity identification purposes. It has been proven that a commercial STR kit joined with supplemental STR loci will strengthen the power of resolving complex paternity cases. In the past years, we encountered two rare and difficult family relationship cases; CODIS-based commercial kits were initially used, but failed. We thus employed additional non-CODIS loci and finally resolved the cases. A total of 40 STRs, 13 CODIS and 27 non-CODIS, were profiled using an ABI 3130 Genetic Analyzer. In the two cases, the exclusionary events and percentage obtained from the non-CODIS markers were 9 (33%, out of 27 in case 1) and 18 (also 33%, out of 54 in case 2). Statistical results revealed that the non-CODIS STRs were significantly informative than the CODIS in identifying problematic kinship cases, and that the high case-resolving capability of the non-CODIS was irrelevant to repeat motif composition of STRs. Four index measures, heterozygosity rate, power of exclusion, power of discrimination, and polymorphism information content, were evaluated for the usefulness of both categories, also implying that the non-CODIS seemed to be more available than the CODIS. Our findings are in concordance with a previous research that the 13 core CODIS STRs are not sufficient to definitively differentiate between pairs of close relatives and may lead to false inclusions. The result, an indispensable system of the non-CODIS, could provide a reference for resolving similar complex relationship cases.

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“…hile X-Plex PCR Amplification Kit is a short tandem repeat (STR) multiplex assay that used to amplifies 13 X-STR loci and an amelogenin locus located on human chromosome X in a single PCR reaction that is DXS8378, DXS9898, DXS8377, HPRTB, GATA172DO5. DXS7423, DXS6809, DXS7132, DXS101, DXS6789, DXS9902, DXS6807 and DXS7424 [8] (Table 1).…”

Section: Introductionmentioning

confidence: 99%

“…Table 1: Allelic Ladder of GenePhile X-Plex Kit with its loci, the relating dyes used, alleles contained in the allelic ladder and the genotype of the Control DNA 9947A and K01 are registered in the table [8] .…”

Section: Introductionmentioning

confidence: 99%

Allele Frequencies of 13 Chromosome X STR in Arab Iraqi Population

2020

IJFMT

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Short tandem repeat (STR) markers are highly being used for identification of human being in addition to paternity and other forensic cases.The X-chromosome STR (X-STR) markers are a powerful supplementary system particularly in deficiency paternity analysis. Several X-linked microsatellites have been assessed but more studies are necessary to verify the population statistics. In our study, we report allele frequencies of 13 X-linked microsatellites (DXS8378,

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False paternity with one or two mismatches using commercial STR kits

Cited by 8 publications

References 18 publications

Maternal allele mutation: Slippage synthesis furnishing evolutionary trend

Maternal allele mutation: Slippage synthesis furnishing evolutionary trend

Non-CODIS DNA markers could be more effective than CODISbased STRs in problematic biological relationship cases

Allele Frequencies of 13 Chromosome X STR in Arab Iraqi Population

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