False-positive imipenemase detected by NG-Test CARBA-5 in carbapenem-resistant
            <i>Acinetobacter baumannii</i>

Bentley, Emily; Russo, Carmella; Khan, Ayesha; Smalc, Shanna; Rhoads, Daniel D.; Humphries, Romney; Tao, Lili

doi:10.1128/spectrum.03757-23

Microbiol Spectr

2024

DOI: 10.1128/spectrum.03757-23

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False-positive imipenemase detected by NG-Test CARBA-5 in carbapenem-resistant Acinetobacter baumannii

Emily Bentley,

Carmella Russo,

Ayesha Khan

et al.

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2024

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Inoculum Size and False-Positive Detection of NDM- and OXA-48-Type Carbapenemases Using Two Multiplex Lateral Flow Assays

Lee,

Cao,

Jiang

et al. 2024

Diagnostics

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The NG-Test CARBA 5 and Carbapenem-resistant K.N.I.V.O. Detection K-Set are lateral flow assays (LFAs) that rapidly detect five carbapenemases (KPC, NDM, IMP, VIM and OXA-48-like). We evaluated the effect of inoculum size on the performance of these two assays using 27 Enterobacterales isolates. Whole-genome sequencing (WGS) was used as the reference method. Using the NG-Test CARBA 5, eight Serratia spp. and six M. morganii isolates showed false-positive NDM results with a high inoculum. Using the Carbapenem-resistant K.N.I.V.O. Detection K-Set, eight M. morganii, four Serratia spp. and one K. pneumoniae isolates showed false-positive NDM and/or OXA-48-like bands at large inoculum sizes, while the other two M. morganii isolates demonstrated false-positive NDM and OXA-48-like results at all inoculum sizes. The false-positive bands varied in intensity. WGS confirmed that no carbapenemase gene was present. No protein sequence with a ≥50% identity to NDM or OXA-48-like enzymes was found. This study emphasizes the importance of assessing inoculum size in the diagnostic evaluation of LFAs.

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Inoculum Size and False-Positive Detection of NDM- and OXA-48-Type Carbapenemases Using Two Multiplex Lateral Flow Assays

Lee,

Cao,

Jiang

et al. 2024

Diagnostics

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show abstract

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False-positive imipenemase detected by NG-Test CARBA-5 in carbapenem-resistant Acinetobacter baumannii

Cited by 1 publication

References 21 publications

Inoculum Size and False-Positive Detection of NDM- and OXA-48-Type Carbapenemases Using Two Multiplex Lateral Flow Assays

Inoculum Size and False-Positive Detection of NDM- and OXA-48-Type Carbapenemases Using Two Multiplex Lateral Flow Assays

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