FAM201A knockdown inhibits proliferation and invasion of lung adenocarcinoma cells by regulating miR‐7515/GLO1 axis

Huang, Jun; Yu, Qiuhua; Zhou, Yanjuan; Chu, Ying; Jiang, Feng; Wang, Qiang

doi:10.1002/jcp.30250

Cited by 12 publications

(20 citation statements)

References 24 publications

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“…For these patients, FAM201A-miR-370-EGFR was suggested as a key axis in regulation of radiotherapy sensitivity [ 34 ]. Another in vivo experiment showed that high level expression of FAM201A involved in development of lung adenocarcinoma and down-regulation of FAM201A exerted the opposite effect [ 35 ]. In addition to cancers, down-regulation of FAM201A was reported to be associated with Osteonecrosis of the femoral head through bioinformatics analysis and quantitative real-time polymerase chain reaction experiments [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

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FAM201A, a long noncoding RNA potentially associated with atrial fibrillation identified by ceRNA network analyses and WGCNA

Chen

Dan

et al. 2022

BMC Med Genomics

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Background Being the most common arrhythmia in clinic, atrial fibrillation (AF) causes various comorbidities to patients such as heart failure and stroke. LncRNAs were reported involved in pathogenesis of AF, yet, little is known about AF-associated lncRNAs. The present study aims to explore lncRNAs associated with AF susceptibility based on competing endogenous RNA (ceRNA) network analysis and weighted gene co-expression network analysis (WGCNA). Methods GSE41177 and GSE79768 datasets were obtained from the Gene Expression Omnibus (GEO) database. Competing endogenous RNA (ceRNA) network analysis was performed using GSE41177. Differentially expressed lncRNAs (DElncRNAs), mRNAs (DEmRNAs) between AF patients and patients with sinus rhythm (SR) were identified from GSE41177 using R software. Then, the ceRNA network was constructed based on DElncRNAs, the predicted target miRNAs and DEmRNAs. Weighted gene co-expression network analysis (WGCNA) was performed using GSE79768 to validate the AF-related lncRNAs identified from GSE41177. LncRNA modules and crucial lncRNAs relevant to AF and were identified. Results In summary, 18 DElncRNAs and 350 DEmRNAs were found between AF patients and SR patients. A total of 5 lncRNAs, 10 miRNAs, and 21 mRNAs were contained in the final ceRNA network. Taking into consideration both the ceRNA theory and inference scores from the comparative toxicogenomics database (CTD) database, the ceRNA axis FAM201A-miR-33a-3p-RAC3 was identified as mostly relevant to AF susceptibility. FAM201A (Gene significance, GS = − 0.62; Module membership, MM = 0.75) was also proved in the blue module, which was identified most highly relevant with AF by WGCNA. Conclusions These results demonstrated that decreased expression of FAM201A might be associated with susceptibility of AF. Working as the ceRNA to regulate RAC3 might be one function of FAM201A in AF susceptibility, which requires further exploration in future research.

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Section: Discussionmentioning

confidence: 99%

“…One of the main functions of FAM201A is to regulate cell proliferation and invasion. Specifically, low expression of FAM201A can inhibit cell proliferation and invasion [ 35 ]. There is evidence that decreased cell proliferation and invasion are related to autophagy activation [ 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

FAM201A, a long noncoding RNA potentially associated with atrial fibrillation identified by ceRNA network analyses and WGCNA

Chen

Dan

et al. 2022

BMC Med Genomics

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“…For these patients, FAM201A-miR-370-EGFR was suggested a key axis in regulation of radiotherapy sensitivity [33]. Another in vivo experiment showed that high level expression of FAM201A involved in development of lung adenocarcinoma and down-regulation of FAM201A exerted the opposite effect [34]. In addition to cancers, down-regulation of FAM201A was reported to be associated with Osteonecrosis of the femoral head through bioinformatics analysis and quantitative real-time polymerase chain reaction experiments [35].…”

Section: Discussionmentioning

confidence: 99%

FAM201A, a Long Noncoding RNA Potentially Associated With Atrial Fibrillation Identified by ceRNA Network Analyses and WGCNA

Chen

Dan

et al. 2021

Preprint

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Background: Atrial fibrillation (AF) is the most common cardiac arrhythmia that contributes to various complications. However, little is known about lncRNAs associated with AF susceptibility. In the present study, we aim to identify lncRNAs involved in pathogenesis of AF based on competing endogenous RNA (ceRNA) network analyses and weighted gene co-expression network analysis (WGCNA).Methods: Two lncRNA and mRNA microarray datasets GSE41177 and GSE79768 were retrieved from the Gene Expression Omnibus (GEO) database. Differentially expressed lncRNAs (DElncRNAs), mRNAs (DEmRNAs) between AF patients and patients with sinus rhythm (SR) were identified from dataset GSE41177. Then, those DElncRNAs associated target miRNAs were predicted. The ceRNA network was constructed based on DElncRNAs, predicted miRNAs and DEmRNAs. To validate the role of AF-related lncRNAs, all lncRNAs form dataset GSE79768 were selected to perform WGCNA. LncRNA modules relevant to AF were identified. Crucial lncRNAs in the module that was most relevant to AF were screened according to the criteria of | Gene significance (GS)| > 0.6 and |Module membership (MM)| > 0.5. Results: A total of 18 DElncRNAs and 350 DEmRNAs were identified between AF patients and SR patients. The final ceRNA network contained 5 lncRNAs, 10 miRNAs, and 21 mRNAs. According to the ceRNA theory, combined with the comparative toxicogenomics database (CTD) database, the ceRNA axis FAM201A-miR-33a-3p-RAC3 was considered associated with AF susceptibility. By WGCNA, the blue module was detected most highly relevant with AF. The lncRNA FAM201A was proved in the blue module and highly related to AF. Conclusions: These results demonstrated that FAM201A might have great potential for susceptibility of AF based on ceRNA network analyses and WGCNA. FAM201A may function, at least partly, as ceRNA to regulate RAC3 in AF susceptibility.

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“…and invasion of cancer cells (10,12,13). However, the role of FaM10a in oa remains poorly understood.…”

Section: Positive Feedback Loop Of Lncrna Fam201a/mir-146a-5p/pou2f1 Regulates Il-1β-induced Chondrocyte Injury In Vitromentioning

confidence: 99%

“…A previous study demonstrated that expression levels of the lncRNA family with sequence similarity 201 member A (FAM201A) are decreased in necrotic femoral head samples ( 11 ). Whereas, FAM201A upregulation has been observed to promote the migration and invasion of cancer cells ( 10 , 12 , 13 ). However, the role of FAM10A in OA remains poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Positive feedback loop of lncRNA FAM201A/miR‑146a‑5p/POU2F1 regulates IL‑1β‑induced chondrocyte injury in vitro

Gan

et al. 2021

Mol Med Rep

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Numerous studies have previously demonstrated that long non-coding RNAs (lncRNAs) serve an important regulatory role in osteoarthritis (OA). In particular, the lncRNA family with sequence similarity 201 member A (FAM201A) was previously found to be downregulated in necrotic femoral head samples. However, the role of FAM201A in IL-1β-induced chondrocyte injury remains unclear. It was hypothesized that FAM201A may exert a protective effect on IL-1β-induced chondrocyte injury in OA by sponging microRNAs (miRNAs/miRs). The purpose of the present study was to explore the role and molecular mechanism of FAM201A in IL-1β-induced chondrocyte injury. A model of OA was established by stimulation C-28/I2 cell with IL-1β in vitro . The expression levels of FAM201A following IL-1β-induced chondrocyte injury were detected via reverse transcription-quantitative PCR. Luciferase reporter assay was used to assess the possible associations among FAM201A, miR-146a-5p and POU class 2 homeobox 1 (POU2F1). Chromatin immunoprecipitation assay was performed to analyze the interaction between POU2F1 and miR-146a-5p. ELISA, TUNEL and western blotting were performed to measure the level of inflammation, lactate dehydrogenase release, apoptosis and the expression of apoptosis-related proteins (Bcl-2, Bax, cleaved caspase 3 and cleaved caspase 9), respectively. The expression levels of FAM201A were found to be downregulated following IL-1β-induced chondrocyte injury. Overexpression of FAM201A exerted a protective effect against IL-1β-induced chondrocyte injury. In addition, FAM201A could upregulate the expression levels of POU2F1 by sponging miR-146a-5p. Further experiments revealed that POU2F1 could bind to the promoter region of FAM201A and subsequently regulate the expression levels of POU2F1, indicating a role for the FAM201A/miR-146a-5p/POU2F1 positive feedback loop in IL-1β-induced chondrocyte injury. The present study revealed the protective effects of the FAM201A/miR-146a-5p/POU2F1 positive feedback loop on IL-1β-induced chondrocyte injury and provided a potential therapeutic target for OA.

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FAM201A knockdown inhibits proliferation and invasion of lung adenocarcinoma cells by regulating miR‐7515/GLO1 axis

Cited by 12 publications

References 24 publications

FAM201A, a long noncoding RNA potentially associated with atrial fibrillation identified by ceRNA network analyses and WGCNA

FAM201A, a long noncoding RNA potentially associated with atrial fibrillation identified by ceRNA network analyses and WGCNA

FAM201A, a Long Noncoding RNA Potentially Associated With Atrial Fibrillation Identified by ceRNA Network Analyses and WGCNA

Positive feedback loop of lncRNA FAM201A/miR‑146a‑5p/POU2F1 regulates IL‑1β‑induced chondrocyte injury in vitro

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