Familial congenital cyanosis caused by Hb-M Yantai (α-76 GAC → TAC, Asp → Tyr)

Abstract

Methemoglobin (Hb-M) is a rare hemoglobinopathy in China. We hereby report on a family living in Yantai, East China, with congenital cyanosis due to Hb-M mutation. The proband, a 65-year-old female, presented 63% oxygen saturation. Both Hb-M concentration and arterial oxygen saturation remained unchanged, even following intravenous treatment with methylene blue. There was also no change in blood-color (chocolate-brown) after adding 0.1% KCN. A fast-moving band (Hb-X) in hemolysates was found by cellulose acetate electrophoresis, the Hb-X/Hb-A ratio exceeding 10%. GT transition at 131nt of exon 2, although present in one of the α2 -globin alleles, was not found in α1 -globin alleles as a whole. This mutation leads to the aspartic acid to tyrosine substitution (Asp76Tyr). In this family, the novel mutation in the α2 -globin gene resulted in a rare form of congenital cyanosis due to Hb-M. This hemoglobin was named Hb-M Yantai .

Genetic cause for congenital methemoglobinemia in an Australian Pomeranian dog

“…Type IV CM is rarely reported and is caused by a deficiency in cytochrome b5 (CYB 5 ) . Abnormal hemoglobins (Hbs) such as Hb‐M and Hb‐Hana also have been reported to cause CM …”