Families of metalloendopeptidases and their relationships

Jiang, Weiping; Bond, Judith S.

doi:10.1016/0014-5793(92)80916-5

Cited by 190 publications

(109 citation statements)

References 31 publications

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“…Such a mutant would take up and process the dipeptides generated by the cocktail of secreted proteases (including LasB elastase, alkaline protease and protease IV) more rapidly. Consistent with this idea, in silico digestion of bovine casein by thermolysin, a homolog of P. aeruginosa LasB elastase with similar cleavage properties (Morihara and Tsuzuki, 1971;Jiang and Bond, 1992), indeed produces up to 6 dipeptides per casein molecule.…”

Section: Resultsmentioning

confidence: 59%

Non-social adaptation defers a tragedy of the commons in Pseudomonas aeruginosa quorum sensing

et al. 2015

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In a process termed quorum sensing (QS), the opportunistic bacterial pathogen Pseudomonas aeruginosa uses diffusible signaling molecules to regulate the expression of numerous secreted factors or public goods that are shared within the population. But not all cells respond to QS signals. These social cheaters typically harbor a mutation in the QS receptor gene lasR and exploit the public goods produced by cooperators. Here we show that non-social adaptation under growth conditions that require QS-dependent public goods increases tolerance to cheating and defers a tragedy of the commons. The underlying mutation is in the transcriptional repressor gene psdR. This mutation has no effect on public goods expression but instead increases individual fitness by derepressing growth-limiting intracellular metabolism. Even though psdR mutant populations remain susceptible to invasion by isogenic psdR lasR cheaters, they bear a lower cheater load than do wild-type populations, and they are completely resistant to invasion by lasR cheaters with functional psdR. Mutations in psdR also sustain growth near wild-type levels when paired with certain partial loss-offunction lasR mutations. Targeted sequencing of multiple evolved isolates revealed that mutations in psdR arise before mutations in lasR, and rapidly sweep through the population. Our results indicate that a QS-favoring environment can lead to adaptations in non-social, intracellular traits that increase the fitness of cooperating individuals and thereby contribute to population-wide maintenance of QS and associated cooperative behaviors.

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Section: Resultsmentioning

confidence: 59%

Non-social adaptation defers a tragedy of the commons in Pseudomonas aeruginosa quorum sensing

et al. 2015

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“…Hence, as pointed out by Jiang and Bond [27], this residue may serve as a label to differentiate between the subfamilies (see Fig. 3).…”

Section: Resultsmentioning

confidence: 89%

Astacins, serralysins, snake venom and matrix metalloproteinases exhibit identical zinc‐binding environments (HEXXHXXGXXH and Met‐turn) and topologies and should be grouped into a common family, the ‘metzincins’

1993

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The X-ray crystal structures of two zmc endopeptldases. astacin from crayfish. and adamalysm II from snake venom, reveal a strong overall lopologlcal equivalence and virtually Identical extended HEXXHXXGXXH zinc-binding segments, but m addition a methlomne-containing turn of similar conformatlon (the 'Met-turn'). which forms a hydrophobic basis for the zmc ion and the three ligandmg hlstidine residues. These two features are also present in a similar arrangement m the matrix metalloproteinases (matrixins) and in the large bacterial Serratra proteinase-like peptidases (serralysins).We suggest that these four proteinases represent members of distinct subfamilies which can be grouped together in a family, for which we propose the designation, metzmcins.Astacm; Snake venom metalloproteinase; Matrix metalloprotemase; Thermolysm; Zmc endopeptidase family: Protein crystal structure

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“…This ZinG~oor~nation site, involving Tyr"', (equivalent to Tyr149 in astacin) is different from other zinc-proteases such as thermolysin and carboxypeptidase A [33,34]. Based on the zinc-binding site, Jiang and Bond [35] have recently proposed a scheme for the classification of met~loproteases into five distinct families, namely thermolysin, astacin, serratia, matrixin and snake venom metalloproteinases. Also conserved in PPHa are four cysteine residues which have been shown to be involved in disulfide-bonding in astacin [S].…”

Section: Discussionmentioning

confidence: 99%

Cloning of the PABA peptide hydrolase alpha subunit (PPHα) from human small intestine and its expression in COS‐1 cells

et al. 1993

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PABA peptide hydrotase (PPH) from human enterocytes is comprised of two subunits, alpha and beta. PPHa is over 70% identical to meprin, a protease isolated from mouse and rat kidney. The enzyme shows a modular organization in that it contains an astacin protease domain, an adhesive domain, an EGF-like domain, and a putative C-terminal membrane spanning domain. Expression of a chimeric meprin-PPHa cDNA in COS-1 cells led to the synthesis of immature, transport-incompetent homodimers. In addition, complex glycosylated forms were detected in the culture medium, suggesting that the enzyme is secreted after proteolytic removal of the membrane anchor.

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Families of metalloendopeptidases and their relationships

Cited by 190 publications

References 31 publications

Non-social adaptation defers a tragedy of the commons in Pseudomonas aeruginosa quorum sensing

Non-social adaptation defers a tragedy of the commons in Pseudomonas aeruginosa quorum sensing

Astacins, serralysins, snake venom and matrix metalloproteinases exhibit identical zinc‐binding environments (HEXXHXXGXXH and Met‐turn) and topologies and should be grouped into a common family, the ‘metzincins’

Cloning of the PABA peptide hydrolase alpha subunit (PPHα) from human small intestine and its expression in COS‐1 cells

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