Objective: To characterise and investigate antimicrobial resistance of Esherichia coli and salmonella strains isolated from indigenous Gallus gallus in a leading slaughterhouse/ market outlet in Nairobi-Kenya. Design: A repeated cross sectional study and based on random sampling was used. Setting: The study was carried out in a leading market outlet in Nairobi, Kenya. Results: A hundred and four indigenous chicken rectal swabs were analysed, of which 67.3% were contaminated with Escherichia coli and 12