Family with Sequence Similarity 83 Member H Promotes the Viability and Metastasis of Cervical Cancer Cells and Indicates a Poor Prognosis

Abstract

Purpose Family with sequence similarity 83 member H (FAM83H) plays key roles in tumorigenesis. However, the specific roles of FAM83H in cervical cancer (CC) have not been well studied. Materials and Methods The RNA-seq data of 306 CC tissues and three normal samples downloaded from The Cancer Genome Atlas were used to analyze the expression of FAM83H. The Kaplan-Meier method was used to draw survival curves. Associations between FAM83H expression and clinicopathological factors were analyzed by chi-square test. Cox proportional hazards model was used to analyze prognostic factors. Loss-of-function assays were conducted to discover the biological functions of FAM83H in cell proliferation, colony formation, invasion, and migration. Real-time Quantitative Reverse Transcription PCR (qRT-PCR) and Western blotting were used to measure the expression levels of FAM83H in CC cell lines. Results Our results demonstrated that FAM83H is overexpressed in CC tissues and that high FAM83H expression is associated with worse overall survival (OS). High FAM83H expression in CC was associated with clinical stage, pathologic tumor, and pathologic node. Univariate analysis suggested that FAM83H expression was significantly related to the OS of CC patients. Although multivariate analysis showed that FAM83H expression was not an independent prognostic factor for the OS of CC patients, the effects of FAM83H on CC cell growth and motility was significant. Loss-of-function experiments demonstrated that knockdown of FAM83H inhibited proliferation, colony formation, migration, and invasion of CC cells by inactivating PI3K/AKT pathway. Conclusion FAM83H might play a crucial role in CC progression and could act as a novel therapeutic target in CC.

Systematic Analysis of Expression Profiles and Prognostic Significance for FAM83 Family in Non-small-Cell Lung Cancer

“…Accordingly, higher mRNA expression of FAM83H was also considerably related with shorter OS and PPS of lung cancer patients. Consistent with our results, Chen et al (2019) demonstrated that FAM83H is increased in cervical cancer tissues and that high FAM83H expression tends to worsen the OS. In addition, suppression of FAM83H refrained proliferation, colony formation, migration, and invasion of CC cells through inactivating PI3K/AKT pathway ( Chen et al, 2019 ).…”

“…Consistent with our results, Chen et al (2019) demonstrated that FAM83H is increased in cervical cancer tissues and that high FAM83H expression tends to worsen the OS. In addition, suppression of FAM83H refrained proliferation, colony formation, migration, and invasion of CC cells through inactivating PI3K/AKT pathway ( Chen et al, 2019 ). Further analysis demonstrated that the patients were categorized by cutoffs of 804 for FAM83H low expression and high expression and achieved a 0.9454545/0.8974359 specificity/sensitivity in distinguishing poor survival from OS.…”

LncRNA FAM83H‐AS1 promotes oesophageal squamous cell carcinoma progression via miR‐10a‐5p/Girdin axis

“…In the present study, FAM83H was found to be up‐regulated and positively correlated with FAM83H‐AS1 expression in ESCC tissues. Moreover, FAM83H functioned as tumour promoter by facilitating oesophageal cancer cells viability and invasion, which was consistent with previous studies in cervical cancer and osteosarcomas 21,22 . Further experiments indicated that FAM83H‐AS1 regulated the expression of FAM83H at transcriptional and post‐transcriptional levels in oesophageal cancer cells.…”

FAM83H and SCRIB stabilize β-catenin and stimulate progression of gastric carcinoma

“…Especially, the combined expression patterns of FAM83H-N and SCRIB-N were strongly predictive of the survival of gastric carcinoma patients. Consistently, FAM83H was an indicator of poor prognosis of cancers of the liver [4], kidney [5], uterus [10,12], and bone [8]. The expression of SCRIB mRNA was also higher in hepatocellular carcinomas compared with non-tumorous liver tissue [20].…”

“…Higher expression of FAM83H increases the proliferation and invasiveness of cancer cells [4,[8][9][10]. The expression of FAM83H is higher in human cancer tissue compared with normal tissue [10][11][12] and higher expression of FAM83H is associated with poor prognosis of cancers of the uterus [10,12], liver [4], kidney [5], and bone [8]. However, FAM83H is a favorable prognostic factor of glioma and head and neck cancers [12].…”