“…Polymerase chain reaction (PCR) was performed as follows: 94°C, 30 seconds; 55–60°C, 1 minute; and 72°C, 1 minute for 40 cycles. Primer sequences for the following genes are published: Angiopoietin‐1 ( Ang‐1 ), Angiopoietin‐2 ( Ang‐2 ), Tie‐1 , Tie‐2 , Flk‐1 and Flt‐1 [5], Flt‐4 [19], Cd34 [20], Endoglin [21], c‐kit [22], Aa4 [23], and Vcam‐1 [24]. We also designed primers for the following: Hhex , 5′‐ATCTCAGAGGATTCCGACCAGG‐3′ forward, 5′‐ATTCCCCAATGTTGCCCCCAC‐3′ reverse (513 bp); Cd133 , 5′‐GGAAAAGTTGCTCTGCGAACC‐3′ forward, 5′‐TGCTTGTTTGCTGGAGGGTC‐3′ reverse (608 bp); Tal1 , 5′‐GCCCAAAGATTTCCCCAATG‐3′ forward, 5′‐AAACCCAGTGCCCCAAACAC‐3′ reverse (543 bp); VE‐Cadherin 5′‐CAGCCAGCATCTTGAACCTG‐3′ forward, 5′‐GAGATTCACGAGCAGTTGGT‐3′ reverse (506 bp) and vWF 5′‐TGTTTTGTGGCGTGTATGTGAGG‐3′ forward, 5′‐GTGTTCTGGGTTTTCTGGAGTTTG‐3′ reverse (584 bp).…”