Far-Red Fluorescent Murine Glioma Model for Accurate Assessment of Brain Tumor Progression

Mishchenko, Tatiana A.; Balalaeva, Irina V.; Klimenko, Maria O.; Brilkina, Anna A.; Peskova, Nina N.; Guryev, Evgenii L.; Krysko, Dmitri V.; Vedunova, Maria

doi:10.3390/cancers14153822

Cited by 3 publications

(1 citation statement)

References 54 publications

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“…The recent development of disruptive high-resolution microscopies contributes to improving the knowledge related to the morphological properties of those cells involved in health and disease. In this framework, epi-fluorescence imaging [ 18 ] and sophisticated volumetric assays by atomic force microscopy [ 19 ] could be employed as a promising tool in the characterization of gangliogliomas. The treatment of choice in humans is total surgical resection based on the slow-growing and non invasive nature of this tumor [ 4 , 5 ].…”

Section: Discussionmentioning

confidence: 99%

First Case of a Cerebrocortical Ganglioglioma in a Dog

Martín

Pumarola

Altuzarra³

et al. 2022

Veterinary Sciences

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Gangliogliomas are extremely rare tumors of the nervous system composed of neoplastic glial and neuronal cells. The aim of the present paper is to describe the clinical presentation, magnetic resonance imaging (MRI) findings and histopathological and immunophenotypical characteristics of a cerebral cortex ganglioglioma in a 7-year-old Border Collie. The dog presented an acute onset of tonic-clonic epileptic seizures. MRI revealed a well-defined large intra-axial mass located on the left forebrain, mainly affecting the frontal cortex. Following humane euthanasia, the histopathological examination of the mass revealed a diffuse proliferation of neoplastic glial cells mixed with anomalous neuronal bodies. Immunohistochemical analyses confirmed the presence of two different populations of neoplastic cells. Most neoplastic glial cells were immunoreactive to glial fibrillary acidic protein (GFAP) and the other subset of neoplastic cells were positive to neuronal markers such as PGP 9.5, synaptophysin (SYN) and neuron-specific enolase (NSE), suggestive of neuronal cells. These findings confirmed the diagnosis of a cerebrocortical ganglioglioma. To the authors knowledge, this is the first description of a ganglioglioma of the cerebral cortex in a dog.

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Section: Discussionmentioning

confidence: 99%

First Case of a Cerebrocortical Ganglioglioma in a Dog

Martín

Pumarola

Altuzarra³

et al. 2022

Veterinary Sciences

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Enhancing glioma treatment with 3D scaffolds laden with upconversion nanoparticles and temozolomide in orthotopic mouse model

Mishchenko,

Klimenko,

Guryev

et al. 2024

Front. Chem.

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Targeted drug delivery for primary brain tumors, particularly gliomas, is currently a promising approach to reduce patient relapse rates. The use of substitutable scaffolds, which enable the sustained release of clinically relevant doses of anticancer medications, offers the potential to decrease the toxic burden on the patient’s organism while also enhancing their quality of life and overall survival. Upconversion nanoparticles (UCNPs) are being actively explored as promising agents for detection and monitoring of tumor growth, and as therapeutic agents that can provide isolated therapeutic effects and enhance standard chemotherapy. Our study is focused on the feasibility of constructing scaffolds using methacrylated hyaluronic acid with additional impregnation of UCNPs and the chemotherapeutic drug temozolomide (TMZ) for glioma treatment. The designed scaffolds have been demonstrated their efficacy as a drug and UCNPs delivery system for gliomas. Using the aggressive orthotopic glioma model in vivo, it was found that the scaffolds possess the capacity to ameliorate neurological disorders in mice. Moreover, upon intracranial co-implantation of the scaffolds and glioma cells, the constructs disintegrate into distinct segments, augmenting the release of UCNPs into the surrounding tissue and concurrently reducing postoperative damage to brain tissue. The use of TMZ in the scaffold composition contributed to restraining glioma development and the reduction of tumor invasiveness. Our findings unveil promising prospects for the application of photopolymerizable biocompatible scaffolds in the realm of neuro-oncology.

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Far-Red Fluorescent Proteins: Tools for Advancing In Vivo Imaging

Shang,

Shao,

Zhao

et al. 2024

Biosensors

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Far-red fluorescent proteins (FPs) have emerged as indispensable tools in in vivo imaging, playing a pivotal role in elucidating fundamental mechanisms and addressing application issues in biotechnology and biomedical fields. Their ability for deep penetration, coupled with reduced light scattering and absorption, robust resistance to autofluorescence, and diminished phototoxicity, has positioned far-red biosensors at the forefront of non-invasive visualization techniques for observing intracellular activities and intercellular behaviors. In this review, far-red FPs and their applications in living systems are mainly discussed. Firstly, various far-red FPs, characterized by emission peaks spanning from 600 nm to 650 nm, are introduced. This is followed by a detailed presentation of the fundamental principles enabling far-red biosensors to detect biomolecules and environmental changes. Furthermore, the review accentuates the superiority of far-red FPs in multi-color imaging. In addition, significant emphasis is placed on the value of far-red FPs in improving imaging resolution, highlighting their great contribution to the advancement of in vivo imaging.

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Far-Red Fluorescent Murine Glioma Model for Accurate Assessment of Brain Tumor Progression

Cited by 3 publications

References 54 publications

First Case of a Cerebrocortical Ganglioglioma in a Dog

First Case of a Cerebrocortical Ganglioglioma in a Dog

Enhancing glioma treatment with 3D scaffolds laden with upconversion nanoparticles and temozolomide in orthotopic mouse model

Far-Red Fluorescent Proteins: Tools for Advancing In Vivo Imaging

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