Farelerde Oksidatif Stres Biyobelirteçlerinin ve Laktik Dehidrogenazin Biyolojik Varyasyonu

Abstract

This is the first report, we aimed to determine the biological variations, analytical quality specifications of oxidative stress biomarkers such as glucose-6-phosphate dehydrogenase (G6PD), catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), reduced glutathione (GSH), malondialdehyde (MDA), and also lactic dehydrogenase (LDH) in mice. In the our study, Mus musculus albino mice, forty-one adult (female 20, male 21), weighing between 23 and 40 g were enrolled for biological variation study during 30-day period. Samples were stored and then tested at the same time. Results were assessed in duplicate and coefficients of variation for each analyte which were isolated to distinguish variation such as within-, and between-individual variations. From these results, an index of individuality were determined for each analyte. The analytical, within-individual, between-individual variations were assessed in apparently healthy mice and were found to be; 1.19%, 22.51% and 7.38% for G6PD, 14.09%, 23.03% and 29.42% for CAT, 6.18%, 11.12% and 21.14% for SOD, 9.13%, 12.00% and 12.58% for GST, 4.12%, 23.40% and 11.20% for GSH, 2.11%, 16.45% and 11.27% for LDH, 4.75%, 9.03% and 31.55% for MDA, respectively. As a result, while population-based reference intervals for G6PD, GSH and LDH were appropriate, Subject-based reference intervals for SOD and MDA were more appropriate. CAT and GST had intermediate individuality so population-based reference intervals should be used with caution. Keywords: Biological variation, lactic dehydrogenase, mice, oxidative stress biomarkers Farelerde Oksidatif Stres Biyobelirteçlerinin ve Laktik Dehidrogenazın Biyolojik Varyasyonu ÖzetBu çalışma, glukoz-6-fosfat dehidrogenaz (G6PD), katalaz (CAT), süperoksit dismutaz (SOD), glutatyon S-transferaz (GST), indirgenmiş glutatyon (GSH), malondialdehit (MDA) gibi oksidatif stres biyobelirteçlerinin ve laktik dehidrogenaz (LDH)'ın biyolojik varyasyonlarını, analitik kalite spesifikasyonlarını ve referans aralıklarını saptamak amacıyla yapılan ilk araştırmadır.