In recent years, the need for alternative energy sources has become an urgent concern due to the decline in fossil fuel input and also the disruption of fossil fuels to cause global warming and waste management, which is a human problem. Biogas, an example of alternative energy, can be used to eliminate these problems related to fossil fuels and biodegradable waste management. Since ancient times, biodegradable waste has been the subject of the discovery of environmentally friendly fuel products such as biogas. Animal manure and urban solid wastes produced by humans are the leading ones among these wastes. In this study, anaerobic digestion of different ratios of cattle manure (CM) and municipal solid waste (MSW) was investigated. Anaerobic digestion studies untreated were carried out in mesophilic conditions. In the reactor where CM: MSW mixture ratio is 2: 1, the highest biogas production (222.5 ± 15.7 mL/g solid matter) was realized compared to other mixture ratios. NaOH pretreatments were applied to this mixing ratio with concentrations of 0.1, 0.5, 1 and 2 N in order to increase biogas yield. After NaOH 1N pretreatment, biogas production increased by 47.46% compared to the untreated reactor. As a result of the application of NaOH pretreatment with 2N value, due to possible rapid resolution, biogas production was decreased compared to 1N NaOH pretreated reactor. As a result of the study, it has been understood that CM and MSW can be a good mixture in anaerobic digestion and biogas production can be increased successfully with NaOH pretreatments.