The study was conducted to identify the cultivation practices followed by the banana growers and compares it with the framework of Good Agricultural Practices as recommended by the World Banana Forum. Both qualitative and quantitative approach was used for data collection. The scheduled interview was carried out from 103 banana growers, two focused group discussion was carried out with the banana growers, and two key informant interview was carried out; one with the President of Banana Growers Association, Chitwan and another with Executive Director of Chamber of Commerce and Industry, Chitwan. The findings revealed that GAP related to soil management and fertilization (87.4%) and harvesting and on-farm processing (94.2%) is adopted at a low level. Only 36% of farmers apply an appropriate dose of chemical fertilizer, 28% follow crop rotation and 7.8% perform mulching/ intercropping. About 38% of the respondents do not have any source of irrigation. About 98% of the farmers do not perform any processing, cleaning, storing, and packaging of banana. Further, 55% of farmers do not use appropriate personal protective equipment while applying pesticides. The results showed that there is low awareness regarding GAP among the banana farmers in the Chitwan district. Therefore, the conduction of awareness programs and training related to GAP is recommended. By far, no study has been carried out to analyze the good agricultural practices applied by banana growers in Nepal. This study identified the prevailing gap in farmer’s practices and good agricultural practices.