Fas/FasL-dependent apoptosis of hepatocytes induced in rat and patients with Clonorchis sinensis infection

Zhang, Xiaoli; Jin, Zhanfeng; Da, Rong; Dong, Yuzhu; Song, Wuqi; Chen, Xiaobei; Huang, Qi; Ling, Hong; Yang, Chunfu; Li, Yihong; Zhang, Fengmin

doi:10.1007/s00436-008-0985-5

Cited by 17 publications

(14 citation statements)

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“…sinensis infection is regarded as an important cause of hepatic fibrosis (Rana et al 2007), which was first associated with liver flukes over 30 years ago (Ch'In et al 1955). Several reports have indicated that the ESP of C. sinensis may play key pathogenic roles in the development of hepatic and bile duct diseases Kim et al 2008Kim et al , 2009aZhang et al 2008). However, the components of the C. sinensis ESP (Li et al 2004;Hu et al 2009a) and their molecular mechanism of pathogenicity remain obscure.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of excretory secretory products from Clonorchis sinensis adult worms: molecular characterization and serological reactivity of a excretory–secretory antigen-fructose-1,6-bisphosphatase

Zheng

Liu

et al. 2011

Parasitol Res

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Clonorchis sinensis is a food-borne zoonotic parasite that resides in bile ducts and causes clonorchiasis, which may result in cholelithiasis, cholecystitis, hepatic fibrosis, and liver tumors. Although total excretory secretory products (ESP) of C. sinensis adults induce hepatic fibrosis in vivo in rats, the causative mechanism is not well understood. To study components of the ESP, C. sinensis culture medium was collected and analyzed using shotgun LC-MS/MS. We identified a total of 110 proteins, including glycometabolic enzymes (such as fructose-1,6-bisphosphatase (FBPase) and enolase), detoxification enzymes (such as glutamate dehydrogenase, dihydrolipoamide dehydrogenase and cathepsin B endopeptidase), and a number of RAB family proteins. To identify a potential causative agent for hepatic fibrosis, we expressed and purified a recombinant FBPase, a 1,041-bp gene product that encodes a 41.7-kDa protein with prototypical FBPase domains and that can form a tetramer with a molecular mass of 166.8 kDa. In addition, we found that FBPase is an antigen present in the ESP and in circulation. Immunofluorescence showed that FBPase localizes to the intestinal cecum and vitellarium in C. sinensis adults. Our results describe the components of the excretory secretory products from C. sinensis adult worms and suggest that FBPase may be an important antigen present in the ESP of C. sinensis and may lay the foundation for additional studies on the development of clonorchiasis-associated hepatic fibrosis.

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Section: Discussionmentioning

confidence: 99%

Proteomic analysis of excretory secretory products from Clonorchis sinensis adult worms: molecular characterization and serological reactivity of a excretory–secretory antigen-fructose-1,6-bisphosphatase

Zheng

Liu

et al. 2011

Parasitol Res

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“…Cell death involving TUNEL-positive hepatocytes was found in tissue around the central vein or portal areas or rats infected with high parasite loads [46]. Whether this cell death was induced by factors released by the parasite or by inflammatory cells is unknown.…”

Section: When Should Cell Death Be Called Apoptosis?mentioning

confidence: 99%

Apoptosis induced by parasitic diseases

2010

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Fatalities caused by parasitic infections often occur as a result of tissue injury that results from a form of host-cell death known as apoptosis. However, instead of being pathogenic, parasite-induced apoptosis may facilitate host survival. Consequently, it is of utmost importance to decipher and understand the process and the role of apoptosis induced or controlled by parasites in humans. Despite this, few studies provide definitive knowledge of parasite-induced host-cell apoptosis. Here, the focus is on a consideration of host-cell apoptosis as either a pathogenic feature or as a factor enabling parasite survival and development.Cell death by apoptotic-like mechanisms could be described as a ride to death with a return ticket, as initiation of the pathway may be reversed, with the potential that it could be manipulated for therapeutic purposes. The management of host-cell apoptosis could thus be an adjunctive factor for parasitic disease treatment. Evidence that the apoptotic process could be reversed by anti-apoptotic drugs has recently been obtained, leading to the possibility of host-cell rescue after injury. An important issue will be to predict the beneficial or deleterious effects of controlling human cell death by apoptotic-like mechanisms during parasitic diseases.

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“…The adult worms inhabit the intrahepatic bile duct and cause clonorchiasis, which is characterized by hyperplasia and metaplasia in the intrahepatic bile duct epithelium [2]. Persistent and chronic infection often lead to hepatobiliary diseases, including cholangitis, cholelithiasis, cholecystitis, pancreatitis, hepatic fibrosis, cholangiocarcinoma and liver cancer [3], [4], [5]. C. sinensis has been classified by the International Agency for Research on Cancer (IARC) as a group 1 biocarcinogen to humans [6].…”

Section: Introductionmentioning

confidence: 99%

A Combination of the Kato-Katz Methods and ELISA to Improve the Diagnosis of Clonorchiasis in an Endemic Area, China

Han

Zhang

et al. 2012

PLoS ONE

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BackgroundExamination of feces by light microscopy is widely used for specific parasitological diagnosis of clonorchiasis. However, the true incidence of infection is underestimated owing to the high missing diagnosis rate of this method. The enzyme-linked immunosorbent assay (ELISA) is widely used for the detection and control of clonorchiasis but the practicality of this method is unclear. The purpose of this study was to evaluate the effect of ELISA as a supplementary method for the diagnosis of clonorchiasis.Methodology/Principal FindingsThe present study recruited 2,359 clinically suspected patients from Heilongjiang Province, China. In all, 954 cases were identified as antibody-positive by immunoglobulin (IgG)-ELISA and 495 individuals were diagnosed as egg-positive by the Kato-Katz (KK) method. The seropositive and egg-negative individuals were re-examined by repeated egg counts and/or the number of KK slides and 18 (18.18%) cases were confirmed as clonorchiasis. The 40.44%, antibody-positive rate determined by IgG-ELISA was significantly higher (P<0.05) than the 21.75% egg-positive rate found by examination of feces. A Bayesian approach indicated that the prevalence of clonorchiasis in this region was 22.27% and that the sensitivity, specificity, positive predictive value and negative predictive value of IgG-ELISA were 98.7%, 76.53%, 54.66% and 99.52%, respectively. The agreement between the two methods was moderate (kappa value = 0.564). The clonorchiasis patients lived mainly along the Songhua River. The risk factors, except for ethnic factors, were estimated effectively by both methods.Conclusions/SignificanceThe present study suggested that clonorchiasis was widely distributed in Heilongjiang Province, China. The missing diagnosis rate was high using the KK technique alone. The combination of immunological methods and parasitological techniques could improve diagnostic accuracy and reduce the missing diagnosis rate. ELISA used as an auxiliary diagnostic method was realistic and practical for a large-scale screening test, monitoring the prevalence and assessing the risk factors of clonorchiasis.

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Fas/FasL-dependent apoptosis of hepatocytes induced in rat and patients with Clonorchis sinensis infection

Cited by 17 publications

References 28 publications

Proteomic analysis of excretory secretory products from Clonorchis sinensis adult worms: molecular characterization and serological reactivity of a excretory–secretory antigen-fructose-1,6-bisphosphatase

Proteomic analysis of excretory secretory products from Clonorchis sinensis adult worms: molecular characterization and serological reactivity of a excretory–secretory antigen-fructose-1,6-bisphosphatase

Apoptosis induced by parasitic diseases

A Combination of the Kato-Katz Methods and ELISA to Improve the Diagnosis of Clonorchiasis in an Endemic Area, China

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