Fascin-1, Ezrin and Paxillin Contribute to the Malignant Progression and Are Predictors of Clinical Prognosis in Laryngeal Squamous Cell Carcinoma

Gao, Wei; Zhang, Chunming; Yan, Feng; Chen, Ganggang; Wen, Shuxin; Hui, Hongxiang; Wang, Binquan

doi:10.1371/journal.pone.0050710

Cited by 33 publications

(38 citation statements)

References 53 publications

(119 reference statements)

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“…Laryngeal squamous cell carcinoma (LSCC) remains one of the most common tumors of the respiratory tract with poor prognosis. We previously found the expression of FSCN1 significantly upregulated in LSCC tissues as compared to adjacent normal tissue . Knockdown of FSCN1 expression suppressed cell migration, invasion, and growth of LSCC .…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometry‐based proteomic analysis of FSCN1‐interacting proteins in laryngeal squamous cell carcinoma cells

et al. 2019

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Fascin actin-bundling protein 1 (FSCN1) is an evolutionarily conserved actinbundling protein that plays a critical role in cell migration, motility, adhesion, and cellular interactions. Although multiple clinical studies have implicated the expression of FSCN1 in laryngeal squamous cell carcinoma (LSCC) progression, the precise mechanism of FSCN1 in the process has not been clearly elucidated. To define FSCN1 function, we characterized FSCN1-interacting proteins in LSCC cells by immunoprecipitation followed by mass spectrometry (MS). After data filtering, 119 proteins with expression in both the Hep-2 and TU-177 cell samples were identified as FSCN1-interacting partners. With in-depth bioinformatics analysis, we linked FSCN1 to critical cellular processes including cell adhesion, glycolysis/gluconeogenesis, regulation of protein ubiquitination, ribosomal RNA processing, and small molecule metabolism. We discuss the interactions between FSCN1 and some of the newly validated partners. The identification of these potential partners of FSCN1 expands our knowledge of the FSCN1 interactome and provides a valuable resource for understanding the functions of this protein in LSCC progression.

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Section: Introductionmentioning

confidence: 99%

Mass spectrometry‐based proteomic analysis of FSCN1‐interacting proteins in laryngeal squamous cell carcinoma cells

et al. 2019

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“…45 More specifically, the fascin promoter has been shown to contain binding sites for TCF/LEF, transcription factors that are activated specifically by β-catenin. 31 To investigate the role of β-catenin/TCF interaction on fascin expression, rat lens epithelial explants were treated with TGF-β2 in the presence and absence of PNU.…”

Section: Resultsmentioning

confidence: 99%

β-Catenin/CBP–Dependent Signaling Regulates TGF-β–Induced Epithelial to Mesenchymal Transition of Lens Epithelial Cells

Taiyab

Korol

Deschamps

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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PurposeTransforming growth factor-β–induced epithelial–mesenchymal transition (EMT) is one of the main causes of posterior capsular opacification (PCO) or secondary cataract; however, the signaling events involved in TGF-β–induced PCO have not been fully characterized. Here, we focus on examining the role of β-catenin/cyclic AMP response element–binding protein (CREB)-binding protein (CBP) and β-catenin/T-cell factor (TCF)-dependent signaling in regulating cytoskeletal dynamics during TGF-β–induced EMT in lens epithelial explants.MethodsRat lens epithelial explants were cultured in medium M199 in the absence of serum. Explants were treated with TGF-β2 in the presence or absence of the β-catenin/CBP interaction inhibitor, ICG-001, or the β-catenin/TCF interaction inhibitor, PNU-74654. Western blot and immunofluorescence experiments were carried out and analyzed.ResultsAn increase in the expression of fascin, an actin-bundling protein, was observed in the lens explants upon stimulation with TGF-β, and colocalized with F-actin filaments. Inhibition of β-catenin/CBP interactions, but not β-catenin/TCF interactions, led to a decrease in TGF-β–induced fascin and stress fiber formation, as well as a decrease in the expression of known markers of EMT, α-smooth muscle actin (α-SMA) and matrix metalloproteinase 9 (MMP9). In addition, inhibition of β-catenin/CBP–dependent signaling also prevented TGF-β–induced downregulation of epithelial cadherin (E-cadherin) in lens explants.ConclusionsWe show that β-catenin/CBP–dependent signaling regulates fascin, MMP9, and α-SMA expression during TGF-β–induced EMT. We demonstrate that β-catenin/CBP–dependent signaling is crucial for TGF-β–induced EMT in the lens.

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“…Head and neck squamous cell carcinoma is one of the most common malignancy worldwide, out of which LSCC has the second highest incidence rate (incidence of LSCC is 2.8/100000 ‐ 5.3/100000), besides it is especially prevalent in the northern areas of China . Approximately 13 430 new cases and 3620 deaths due to LSCC have been estimated in the United States alone .…”

Section: Introductionmentioning

confidence: 99%

Identification of miR‐145‐5p‐Centered Competing Endogenous RNA Network in Laryngeal Squamous Cell Carcinoma

et al. 2019

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This study intends to investigate the transcriptional regulatory role of miR‐145‐5p in laryngeal squamous cell carcinoma (LSCC). LSCC cell line TU‐177 is transfected with miR‐145‐5p mimics, generating miR‐145‐5p‐overexpression LSCC cells. Whole transcriptome microarrays are used to investigate the differentially expressed lncRNAs, circRNAs, mRNAs, and miRNAs. The target genes of miRNAs are predicted and performed functional annotation. Additionally, the circRNAs, lncRNAs, and mRNAs that interact with miRNAs are predicted, and then the competing endogenous RNAs (ceRNAs) are predicted. Microarray analysis identifies 26 miRNAs, 248 mRNAs, 1118 lncRNAs, and 382 circRNAs differentially expressed in miR‐145‐5p overexpressed LSCC cells. Overall, 675 target genes are identified for the differentially expressed miRNAs, which involved in cell adhesion associated gene ontology (GO) terms, and MAPK and FoxO signaling pathways. The up‐regulated mRNAs involved in the pathway of ABC transporters, while the down‐regulated mRNAs involved in pathway of olfactory transduction. Moreover, 149 ceRNAs are predicted, which are associated with apoptosis, Wnt pathway, and metabolic pathway. Furthermore, qPCR results confirm that miR‐145‐5p affects expression of lncRNAs, miRNAs, mRNAs, and circRNAs in LSCC cells. Collectively, miR‑145‑5p may be inhibits LSCC progression via ceRNA‐mediated pathways, such as WNT2B‐miR‐145‐5p‐NONHSAT127539.2, CASP10‐miR‐145‐5p‐NONHSAT127539.2, CASP10‐miR‐145‐5p‐circ_0003519, and TPO‐miR‐145‐5p‐circ_0003519.

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Fascin-1, Ezrin and Paxillin Contribute to the Malignant Progression and Are Predictors of Clinical Prognosis in Laryngeal Squamous Cell Carcinoma

Cited by 33 publications

References 53 publications

Mass spectrometry‐based proteomic analysis of FSCN1‐interacting proteins in laryngeal squamous cell carcinoma cells

Mass spectrometry‐based proteomic analysis of FSCN1‐interacting proteins in laryngeal squamous cell carcinoma cells

β-Catenin/CBP–Dependent Signaling Regulates TGF-β–Induced Epithelial to Mesenchymal Transition of Lens Epithelial Cells

Identification of miR‐145‐5p‐Centered Competing Endogenous RNA Network in Laryngeal Squamous Cell Carcinoma

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