Fasciola gigantica: Anthelmintic effect of the aqueous extract of Artocarpus lakoocha

Saowakon, Naruwan; Tansatit, Tawewan; Wanichanon, Chaitip; Chanakul, Waraporn; Reutrakul, Vichai; Sobhon, Prasert

doi:10.1016/j.exppara.2009.04.011

Cited by 47 publications

(38 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Relative Motility (RM) values is indicated to assess the motility and viability of flukes to determine the mortality pattern of trematode model under study [11,37,38]. A smaller RM values indicates higher mortality rate.…”

Section: Discussionmentioning

confidence: 99%

“…The trials to using plants as anthelmintic remedies go back to older methods of the pre chemotherapeutical periods; however they have become more and more important today [8]. Several plants have been tested for their anthelmintic efficacies [9][10][11][12][13][14][15][16][17][18], against trematode infection. To date, there has been no literature cited to show the anthelmintic property of Marattia fraxinea Sm., lower vascular cryptogamic plants scientifically investigated to establish whether or not they have antitrematodal properties in vitro.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gastrothylax crumenifer: Ultrastructure and histopathology study of in vitro trematodicidal action of Marattia fraxinea (Sm.)

et al. 2017

View full text Add to dashboard Cite

Background: Trematodicidal action of Marattia fraxinea (Sm.) rhizome, extracts against adult Gastrothylax crumenifer and its morphological changes under light and scanning electron microscope was studied in this work. This plant according to folk medicine has been reported to be used as antioxidant, antimicrobial and anthelmintic against ankylostomiasis. Methods: Preliminary screening involved the qualitative methods to detect the presence tannins, saponins, quinones, terpenoids, steroids, flavonoids, phenol, alkaloids, glycosides, cardiac glycosides, coumarins, anthocyanin and betacyanin. Total phenol, total terpenoids, total tannin and total flavonoids were quantitatively estimated. Total phenolic content was estimated by Folin-Ciocalteau method. Total flavonoid content was determined by the aluminium chloride colorimetric method. In-vitro incubation study of Marattia fraxinea Sm. extracts against Gastrothylax crumenifer were performed using 25 ml of Hedon-Fleig (H-F) salt solution containing various concentrations (1, 2, 3, 4 and 5 mg/ml) respectively as test extracts, positive control Oxyclozanide @ 1% (250 mg/25 ml) and negative control H-F salt solution, distributed to 7-Petri plates in an incubator with 5% CO 2 at 37°C. Twenty five amphistomes were incubated and the motility of control and test group was observed under dissection microscope at a regular time interval of (0, 10, 15, 30 and 60) min respectively. The motility response of the parasites was categorized with specific score 3, 2, 1, 0 respectively. Relative Motility (RM) value and Lethal Concentration 50 (LC 50 ) were determined by probit regression analysis. Ultrastructure and histopathological changes on morphology of G.crumenifer were interpreted. The bioactive compounds were analysed using gas chromatography-mass spectrometry (GC-MS) instrument. Results: Phytochemical analysis of M.fraxinea (Sm.) revealed the presence of secondary metabolites tannins, saponins, quinones, terpenoids, steroids, flavonoids, phenol, alkaloids, cardiac glycosides, coumarins, and betacyanin. Total terpenoid, total phenol, total tannin and total flavonoid content were found to be 16.00 ± 0.85 mg/g. 5.40 ± 0.71) mg GAE/g, 7.90 ± 0.24 mg TAE/g and 1.25 ± 0.41 mg QE/g respectively. GC-MS analysis showed presence of PHYTOL, a diterpenoids, fern-8-ene, a triterpenoids shown to have potent anthelmintic property. In vitro incubation study revealed death of all trematodes, lethal at 60 min incubation time at 5 mg/ml concentration, indicated Relative Motility (RM) value is 0, least effective compared to positive control Oxyclozanide, where RM value is 0 at 10 min. Lethal concentration 50 (LC 50 ) was found to be 4.873 mg/ml. Confirmative study on gross morphology, histopathology, and ultra-structural changes showed considerable effect on suckers, teguments and internal organs and were dose dependent. Conclusion: It is indicative that presence of terpenoids, flavonoids, tannins and phenolics which are contributing factors for anthelmintic activity, producing various ...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gastrothylax crumenifer: Ultrastructure and histopathology study of in vitro trematodicidal action of Marattia fraxinea (Sm.)

et al. 2017

View full text Add to dashboard Cite

show abstract

“…At present, effective and commercial vaccines are not yet available [7] , therefore anthelmintic drugs are the main method employed for controlling the fluke infection. Triclabendazole (TCBZ) is the drug of choice and the most effective flukicide against both juvenile and adult flukes.…”

Section: Introductionmentioning

confidence: 99%

Effects of Mirazid® and myrrh volatile oil on adult Fasciola gigantica under laboratory conditions

Am¹,

Shalaby²,

El-Khateeb³

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

View full text Add to dashboard Cite

“…OR showed inhibitory effect on the growth of HSV-1 and HSV-2 wild types, drug-resistant HSV-1 strains (12), clinical isolates of HSV-1 and HSV-2 (13), as well as various varicella zoster virus (VZV) strains including wild type, thymidine kinase-deficient and DNA polymerase VZV mutant in vitro (14). Various biological activities including tyrosinaseinhibitor (15), antioxidant (16) and anthelmintic activities (17) have been also reported. As previously reported, 30% w/w of OR in Vaseline topically applied five times daily provided better therapeutic efficacy than the oral treatment of OR in cutaneous HSV-1 infection in mice (12).…”

Section: Introductionmentioning

confidence: 99%

Microemulsion-Based Oxyresveratrol for Topical Treatment of Herpes Simplex Virus (HSV) Infection: Physicochemical Properties and Efficacy in Cutaneous HSV-1 Infection in Mice

et al. 2012

View full text Add to dashboard Cite

Abstract. The physicochemical properties of the optimized microemulsion and the permeating ability of oxyresveratrol in microemulsion were evaluated, and the efficacy of oxyresveratrol microemulsion in cutaneous herpes simplex virus type 1 (HSV-1) infection in mice was examined. The optimized microemulsion was composed of 10% w/w of isopropyl myristate, 35% w/w of Tween 80, 35% w/w of isopropyl alcohol, and 20% w/w of water. The mean particle diameter was 9.67±0.58 nm, and the solubility of oxyresveratrol in the microemulsion was 196.34±0.80 mg/ml. After accelerated and long-term stability testing, the microemulsion base and oxyresveratrol-loaded microemulsion were stable. The cumulative amount of oxyresveratrol permeating through shed snake skin from microemulsion at 6 h was 93.04 times compared to that of oxyresveratrol from Vaseline, determined at 20% w/w concentration. In cutaneous HSV-1 infection in mice, oxyresveratrol microemulsion at 20%, 25%, and 30% w/w, topically applied five times daily for 7 days after infection, was significantly effective in delaying the development of skin lesions and protecting from death (p<0.05) compared with the untreated control. Oxyresveratrol microemulsion at 25% and 30% w/w was significantly more effective than that of 30% w/w of oxyresveratrol in Vaseline (p<0.05) and was as effective as 5% w/w of acyclovir cream, topically applied five times daily (p>0.05). These results demonstrated that topical oxyresveratrol microemulsion at 20-30% w/w was suitable for cutaneous HSV-1 mouse infection.

show abstract

Fasciola gigantica: Anthelmintic effect of the aqueous extract of Artocarpus lakoocha

Cited by 47 publications

References 28 publications

Gastrothylax crumenifer: Ultrastructure and histopathology study of in vitro trematodicidal action of Marattia fraxinea (Sm.)

Gastrothylax crumenifer: Ultrastructure and histopathology study of in vitro trematodicidal action of Marattia fraxinea (Sm.)

Effects of Mirazid® and myrrh volatile oil on adult Fasciola gigantica under laboratory conditions

Microemulsion-Based Oxyresveratrol for Topical Treatment of Herpes Simplex Virus (HSV) Infection: Physicochemical Properties and Efficacy in Cutaneous HSV-1 Infection in Mice

Contact Info

Product

Resources

About