Fast activated charcoal prepurification of Fusarium solani β-glucosidase for an efficient oleuropein bioconversion

Abstract

Fungal β-glucosidases were extensively studied regarding their various potential biotechnology applications. Here, we report the selection of Fusarium solani strain producing high yield of β-glucosidase activity. The effect of some factors on β-glucosidase production was studied including: Initial pH, medium composition, concentration of carbon and nitrogen sources, and particle size of raw substrates. The optimal enzyme production was obtained with 4 units of pH. The highest β-glucosidase activity was produced on 4% wheat bran (WB) as raw carbon sources, reaching 5 U/mL. A positive correlation between WB particle size and the β-glucosidase production level was settled. The last one was enhanced to 13.60 U/mL in the presence of 0.5% (w/v) of ammonium sulfate. Interestingly, the activated charcoal was used as an inexpensive reagent enabling a rapid and efficient purification prior step that improved the enzyme-specific activity. Eventually, F. solani β-glucosidase acts efficiently during the bioconversion process of oleuropein. Indeed, 82.5% of oleuropein was deglycosylated after 1 hr at 40°C. Altogether, our data showed that the β-glucosidase of F. solani has a potential application to convert oleuropein to ameliorate food quality.

Hotspot Wizard‐informed engineering of a hyperthermophilic β‐glucosidase for enhanced enzyme activity at low temperatures

“…The conversion of cellulosic biomass into biofuel holds the promise for the development of sustainable energy with minimal carbon emissions (Akram et al, 2021). In addition to the renewable energy application, the hydrolysis of cellulosic biomass produces various chemicals and their intermediates used in pharmaceutical, textile, and food industries (Boudabbous et al, 2017; Erkanli et al, 2024; Li et al, 2021; Yan et al, 2016; Zada et al, 2021). Due to its rigid structure, cellulosic biomass usually undergoes physical or chemical pretreatments, such as milling, heating, and acid/alkaline treatments (Mankar et al, 2021), to make cellulose become more accessible and amenable to enzymatic or microbial degradation (Mankar et al, 2021).…”