Fast and automated large-area imaging MALDI mass spectrometry in microprobe and microscope mode

Klerk, Leendert A.; Altelaar, Maarten; Froesch, Martin; McDonnell, Liam A.; Heeren, Ron M. A.

doi:10.1016/j.ijms.2009.02.032

Cited by 38 publications

(45 citation statements)

References 23 publications

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“…MALDI IMS technology has rapidly progressed over the past decade, with significant improvements seen in instrumentation [10-12], laser technology [13-15], and sample preparation [16-24] techniques. These advances provide for increased sensitivity, reduced acquisition time, and greater spatial resolution.…”

Section: Introductionmentioning

confidence: 99%

High Resolution MALDI Imaging Mass Spectrometry of Retinal Tissue Lipids

Anderson

Ablonczy

Koutalos

et al. 2014

J. Am. Soc. Mass Spectrom.

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Matrix assisted laser desorption ionization imaging mass spectrometry (MALDI IMS) has the ability to provide an enormous amount of information on the abundances and spatial distributions of molecules within biological tissues. The rapid progress in the development of this technology significantly improves our ability to analyze smaller and smaller areas and features within tissues. The mammalian eye has evolved over millions of years to become an essential asset for survival, providing important sensory input of an organism’s surroundings. The highly complex sensory retina of the eye is comprised of numerous cell types organized into specific layers with varying dimensions, the thinnest of which is the 10 μm retinal pigment epithelium (RPE). This single cell layer and the photoreceptor layer contain the complex biochemical machinery required to convert photons of light into electrical signals that are transported to the brain by axons of retinal ganglion cells. Diseases of the retina including age related macular degeneration (AMD), retinitis pigmentosa, and diabetic retinopathy occur when the functions of these cells are interrupted by molecular processes that are not fully understood. In this report, we demonstrate the use of high spatial resolution MALDI IMS and FT-ICR tandem mass spectrometry in the Abca4−/− knockout mouse model of Stargardt disease, a juvenile onset form of macular degeneration. The spatial distributions and identity of lipid and retinoid metabolites are shown to be unique to specific retinal cell layers.

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Section: Introductionmentioning

confidence: 99%

High Resolution MALDI Imaging Mass Spectrometry of Retinal Tissue Lipids

Anderson

Ablonczy

Koutalos

et al. 2014

J. Am. Soc. Mass Spectrom.

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“…An alternative approach is microscope MALDI-MSI, which employs a defocused UV laser to envelop a large matrix-coated area. Then, ion optics transfer and magnify the ion packet produced by the single laser shot, relying on a position-sensitive detector to record the resulting 2D images of ion distribution [25,26]. The high spatial resolution, increased speed and greater versatility of potential ion sources offered by the microscope approach appear to have potential.…”

Section: Methodsological Developmentsmentioning

confidence: 99%

“…As mentioned before, the spatial resolution of DESI is approximately 180–220 μm, which is comparable to that of LAESI but surpassed by SIMS for small-molecule analysis [47,57]. Solutions are currently being undertaken to increase this resolution, such as using the microscope rather than microprobe imaging mode and examining restricted regions at high spatial resolutions with fine needles that remove material to be examined in the open environment [26,56,64]. …”

Section: Methodsological Developmentsmentioning

confidence: 99%

From Pixel to Voxel: A Deeper View of Biological Tissue by 3D Mass Spectral Imaging

Greer

2011

Bioanalysis

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Three dimensional mass spectral imaging (3D MSI) is an exciting field that grants the ability to study a broad mass range of molecular species ranging from small molecules to large proteins by creating lateral and vertical distribution maps of select compounds. Although the general premise behind 3D MSI is simple, factors such as choice of ionization method, sample handling, software considerations and many others must be taken into account for the successful design of a 3D MSI experiment. This review provides a brief overview of ionization methods, sample preparation, software types and technological advancements driving 3D MSI research of a wide range of low- to high-mass analytes. Future perspectives in this field are also provided to conclude that the positive and promises ever-growing applications in the biomedical field with continuous developments of this powerful analytical tool.

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“…Lastly, it is worth noting that instruments operating in mass microscope mode would also offer the opportunity for faster analysis. The desorption/ionization beam in this configuration is 200–300 μm, so fewer measurement points are required for a given area, even for high resolution images [22]. The disadvantage to such an approach is the extremely limited m/z range and sensitivity of these instruments relative to more conventional mass analyzers.…”

Section: Maldi Ims As a Robust Bioanalytical Toolmentioning

confidence: 99%

MALDI imaging mass spectrometry: Spatial molecular analysis to enable a new age of discovery

Gessel

Norris

Caprioli

2014

Journal of Proteomics

245

204

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Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) combines the sensitivity and selectivity of mass spectrometry with spatial analysis to provide a new dimension for histological analyses to provide unbiased visualization of the arrangement of biomolecules in tissue. As such, MALDI IMS has the capability to become a powerful new molecular technology for the biological and clinical sciences. In this review, we briefly describe several applications of MALDI IMS covering a range of molecular weights, from drugs to proteins. Current limitations and challenges are discussed along with recent developments to address these issues.

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Fast and automated large-area imaging MALDI mass spectrometry in microprobe and microscope mode

Cited by 38 publications

References 23 publications

High Resolution MALDI Imaging Mass Spectrometry of Retinal Tissue Lipids

High Resolution MALDI Imaging Mass Spectrometry of Retinal Tissue Lipids

From Pixel to Voxel: A Deeper View of Biological Tissue by 3D Mass Spectral Imaging

MALDI imaging mass spectrometry: Spatial molecular analysis to enable a new age of discovery

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