Fast and Cost-Effective Isolation of Circulating Exosomes Using Porous PDMS-Based Microsystem(Porous Exochip)

Kang, Yoon‐Tae; Marvar, Joseph; Onukwugha, Nna-Emeka; Raju, Kruthi Srinivasa; Niu, Zeqi; Rupp, Brittany; Fortna, Shawn; Nagrath, Sunitha

doi:10.1109/mems51670.2022.9699531

Cited by 3 publications

(4 citation statements)

References 16 publications

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“…Kang et al proposed a biofunctionalized microsystem made of porous PDMS material. 73 Highly sensitive cancer exosomes can be separated from other exosomes thanks to the particular affinity between cancer-related exosomes and Annexin V on porous PDMS, and the concentration of captured exosomes is higher than that of traditional gold standard ultracentrifugation separation. The concentration of isolated exosomes measured by the porous chip was twice that of the ultracentrifugation method.…”

Section: Filtration-basedmentioning

confidence: 99%

“…Microfluidic technology has a very significant advantage in detecting different exosomes. It can accurately detect the number of exosome particles, surface proteins, internal RNA, and other information. , More and more researchers have reported many kinds of cancer-related proteins and nucleic acid in exosomes such as EpCAM, GPC-1, CA24, etc . Traditional methods for exosome analysis require a series of pretreatment steps such as exosome lysis and nucleic acid extraction .…”

Section: Plasmonic Biosensor For Exosome Detectionmentioning

confidence: 99%

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Exosome Isolation and Detection: From Microfluidic Chips to Nanoplasmonic Biosensor

Shen,

Ma,

et al. 2024

ACS Appl. Mater. Interfaces

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Exosomes are becoming more widely acknowledged as significant circulating indicators for the prognosis and diagnosis of cancer. Circulating exosomes are essential to the development and spread of cancer, according to a growing body of research. Using existing technology, characterizing exosomes is quite difficult. Therefore, a direct, sensitive, and targeted approach to exosome detection will aid in illness diagnosis and prognosis. The review discusses the new strategies for exosome isolation and detection technologies from microfluidic chips to nanoplasmonic biosensors, analyzing the advantages and limitations of these new technologies. This review serves researchers to better understand exosome isolation and detection methods and to help develop better exosome isolating and detecting devices for clinical applications.

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Section: Filtration-basedmentioning

confidence: 99%

Section: Plasmonic Biosensor For Exosome Detectionmentioning

confidence: 99%

Exosome Isolation and Detection: From Microfluidic Chips to Nanoplasmonic Biosensor

Shen,

Ma,

et al. 2024

ACS Appl. Mater. Interfaces

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“…In light of this, the Nagrath group has made considerable progress in addressing the scalability issue through the development of both the ExoSponge and ExoBead, two devices which boast simple, quick, and affordable manufacturing. 93,94 Specificity and preprocessing continue to be hurdles as developing devices make efforts towards immunoaffinity and size-based isolations. 95 Yeo et al contributed to solving these issues by introducing a label-free centrifugal microfluidic device, μCENSE.…”

Section: Size-exclusion Chromatography (Sec)mentioning

confidence: 99%

Emerging micro-nanotechnologies for extracellular vesicles in immuno-oncology: from target specific isolations to immunomodulation

2022

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“…Although ultracentrifugation (UC) is the gold standard for EV isolation, it is labour‐intensive, time‐consuming and can potentially damage EVs due to high g forces used during separation (Jeppesen et al., 2014 ). To address these limitations, various technologies based on size, affinity, nanomaterials, fluid chips and polymers have been developed for efficient EV separation (Contreras‐Naranjo et al., 2017 ; Dao et al., 2022 ; Deregibus et al., 2016 ; Kang et al., 2022 , Kim & Shin, 2021 ; Kim et al., 2020 ; Pammi Guru et al., 2022 ). For instance, the methodology of protamine‐based EV isolation has been previously described in our previous studies (Kim & Shin, 2021 ; Kim et al., 2020 ) and others (Deregibus et al., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

An innovative charge‐based extracellular vesicle isolation method for highly efficient extraction of EV‐miRNAs from liquid samples: miRQuick

Park,

Bae,

Seong

et al. 2023

J of Extracellular Bio

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Extracellular vesicle‐derived microRNAs (EV‐miRNAs) are promising biomarkers for early cancer diagnosis. However, existing EV‐miRNA extraction technologies have a complex two‐step process that results in low extraction efficiency and inconsistent results. This study aimed to develop and evaluate a new single‐step extraction method, called miRQuick, for efficient and high‐recovery extraction of EV‐miRNAs from samples. The miRQuick method involves adding positively charged substances to the sample, causing negatively charged EVs to quickly aggregate and precipitate. A membrane lysate is then added to extract only miRNA. The entire process can be completed within an hour using standard laboratory equipment. We validated the miRQuick method using various analytical techniques and compared its performance to other methods for plasma, urine and saliva samples. The miRQuick method demonstrated significantly higher performance than other methods, not only for blood plasma but also for urine and saliva samples. Furthermore, we successfully extracted and detected nine biomarker candidate miRNAs in the plasma of breast cancer patients using miRQuick. Our results demonstrate that miRQuick is a rapid and efficient method for EV‐miRNA extraction with excellent repeatability, making it suitable for various applications including cancer diagnosis.

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Fast and Cost-Effective Isolation of Circulating Exosomes Using Porous PDMS-Based Microsystem(Porous Exochip)

Cited by 3 publications

References 16 publications

Exosome Isolation and Detection: From Microfluidic Chips to Nanoplasmonic Biosensor

Exosome Isolation and Detection: From Microfluidic Chips to Nanoplasmonic Biosensor

Emerging micro-nanotechnologies for extracellular vesicles in immuno-oncology: from target specific isolations to immunomodulation

An innovative charge‐based extracellular vesicle isolation method for highly efficient extraction of EV‐miRNAs from liquid samples: miRQuick

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