Fast and efficient size-based separations of polymers using ultra-high-pressure liquid chromatography

Uliyanchenko, Elena; Schoenmakers, Peter J.; Wal, Sj. van der

doi:10.1016/j.chroma.2011.01.053

Cited by 63 publications

(47 citation statements)

References 49 publications

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“…On the other hand, 2 D SEC potentially suffers from limited resolution. However, recent studies have shown the potential of fast SEC, using UHPLC technology [126,127]. More recently, the use of core-shell particles in 2 D SEC to improve resolution was demonstrated [128] and this was later confirmed [129].…”

Section: Size-exclusion Chromatography and Hydrodynamic Chromatographymentioning

confidence: 96%

Optimizing separations in online comprehensive two‐dimensional liquid chromatography

Pirok

Gargano

Schoenmakers

2017

J of Separation Science

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204

139

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Online comprehensive two‐dimensional liquid chromatography has become an attractive option for the analysis of complex nonvolatile samples found in various fields (e.g. environmental studies, food, life, and polymer sciences). Two‐dimensional liquid chromatography complements the highly popular hyphenated systems that combine liquid chromatography with mass spectrometry. Two‐dimensional liquid chromatography is also applied to the analysis of samples that are not compatible with mass spectrometry (e.g. high‐molecular‐weight polymers), providing important information on the distribution of the sample components along chemical dimensions (molecular weight, charge, lipophilicity, stereochemistry, etc.). Also, in comparison with conventional one‐dimensional liquid chromatography, two‐dimensional liquid chromatography provides a greater separation power (peak capacity). Because of the additional selectivity and higher peak capacity, the combination of two‐dimensional liquid chromatography with mass spectrometry allows for simpler mixtures of compounds to be introduced in the ion source at any given time, improving quantitative analysis by reducing matrix effects. In this review, we summarize the rationale and principles of two‐dimensional liquid chromatography experiments, describe advantages and disadvantages of combining different selectivities and discuss strategies to improve the quality of two‐dimensional liquid chromatography separations.

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Section: Size-exclusion Chromatography and Hydrodynamic Chromatographymentioning

confidence: 96%

Optimizing separations in online comprehensive two‐dimensional liquid chromatography

Pirok

Gargano

Schoenmakers

2017

J of Separation Science

Self Cite

204

139

View full text Add to dashboard Cite

show abstract

“…Thus, particle size of adsorbents used in SEC was reduced from conventional 10-20 m to 3-5 m [8, Abbreviations: PE3A, pentaerythritol triacrylate; PE4A, pentaerythritol tetraacrylate; UHP-SEC, ultra-high performance size exclusion chromatography www.gelifesciences.com/protein-purification, www.tosohbioscience.com] and then even further to less than 2 m [9,10]. Reduction of the size of the particles increases the column efficiency and resolution but requires high-pressure instrumentation and high mechanical stability of the packings.…”

Section: Introductionmentioning

confidence: 99%

Monolithic capillary columns based on pentaerythritol acrylates for molecular‐size‐based separations of synthetic polymers

Kurganov

Victorova

Kanat’eva

2015

J of Separation Science

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Monolithic capillary columns based on pentaerythritol triacrylate and pentaerythritol tetraacrylate were synthesized using different compositions of polymerization mixtures and different polymerization conditions. The impact of porogen type and porogen/monomer ratio on the porosity of synthesized monoliths was investigated. Porogen type appears to be the main factor influencing the separating properties of the monolithic sorbent. Using optimal polymerization conditions (porogen type, porogen/monomer ratio, reaction temperature, time etc.) monoliths with a porous structure optimized for polymer separations can be obtained. The monolithic capillary columns containing porous sorbents with optimized porosity are capable of separating 10 to 12 polystyrene standards in one chromatographic run utilizing both size exclusion chromatography and hydrodynamic chromatography separation mechanisms.

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“…For SEC, these sub-2 μm materials have been introduced several years ago; however, their application in speciation studies is novel. A recent review reports on developments in UHPLC SEC [39] including the key application areas such as, e.g., purity monitoring of protein-related compounds in industry, the development process of biotherapeutic proteins, the field of proteomics, and the polymer industry [40,41]. To the best of our knowledge, this technology has not been applied to speciation analysis with ICP-MS detection so far.…”

Section: Introductionmentioning

confidence: 99%

Critical assessment of different methods for quantitative measurement of metallodrug-protein associations

et al. 2018

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Quantitative screening for potential drug–protein binding is an essential step in developing novel metal-based anticancer drugs. ICP–MS approaches are at the core of this task; however, many applications lack in the capability of large-scale high-throughput screenings and proper validation. In this work, we critically discuss the analytical figures of merit and the potential method-based quantitative differences applying four different ICP–MS strategies to ex vivo drug–serum incubations. Two candidate drugs, more specifically, two Pt(IV) complexes with known differences of binding affinity towards serum proteins were selected. The study integrated centrifugal ultrafiltration followed by flow injection analysis, turbulent flow chromatography (TFC), and size exclusion chromatography (SEC), all combined with inductively coupled plasma-mass spectrometry (ICP–MS). As a novelty, for the first time, UHPLC SEC-ICP–MS was implemented to enable rapid protein separation to be performed within a few minutes at > 90% column recovery for protein adducts and small molecules. Graphical abstractQuantitative screening for potential drug–protein binding is an essential step in developingnovel metal-based anticancer drugs Electronic supplementary materialThe online version of this article (10.1007/s00216-018-1328-8) contains supplementary material, which is available to authorized users.

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Fast and efficient size-based separations of polymers using ultra-high-pressure liquid chromatography

Cited by 63 publications

References 49 publications

Optimizing separations in online comprehensive two‐dimensional liquid chromatography

Optimizing separations in online comprehensive two‐dimensional liquid chromatography

Monolithic capillary columns based on pentaerythritol acrylates for molecular‐size‐based separations of synthetic polymers

Critical assessment of different methods for quantitative measurement of metallodrug-protein associations

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