Fast and environmentally friendly determination of salicylic acid in plant materials by sequential injection chromatography

Abstract

An improved procedure for determination of salicylic acid in plant materials is proposed.

Flow-Batch Sample Preparation for Fractionation of the Stress Signaling Phytohormone Salicylic Acid in Fresh Leaves

“…A 0.8 mol L −1 acetate buffer solution (pH 5.0) was prepared from sodium acetate (Sigma-Aldrich, USA) with pH adjusted to 5.0 with 1.0 mol L −1 HCl solution. The mobile phase for the chromatographic separation was a 7% v/v acetonitrile in 0.2 mol L −1 acetate buffer solution (pH 5.0) [ 16 ].…”

“…Crude and hydrolyzed extracts were analyzed with the SIC system ( Figure 1(b) ) as described by Barrientos et al [ 16 ]. Initially, 3.9 mL of the mobile phase was aspirated to HC2 at 50 μ L s −1 followed by 10 μ L of the extract.…”

“…us, the flow-batch system, under the optimized conditions, is also robust in relation to the extraction step. For accuracy assessment, sugarcane, corn, and soybean leaves previously analyzed by batch procedures for free [16] and total SA [18] determination were assayed by the flowbatch sample preparation procedure (Table 3). e mean values agreed and the variances were not significantly different, according to statistical tests (F-test and paired t-test) both at the 95% confidence level.…”

“…Despite the lower chromatographic efficiency compared to HPLC, successful separations have been achieved for a diversity of applications, including determination of pharmaceutical compounds in medicines [12] and body fluids [14], contaminants in food [13], and pesticides in natural waters [15]. Barrientos et al [16] successfully applied SIC with fluorimetric detection for determination of free SA on plant leaves after batchwise microwave-assisted extraction. It was demonstrated that fluorimetry based on the intrinsic fluorescence of salicylate is a cost-effective alternative to the MS detectors.…”

An overview of the Brazilian contributions to Green Analytical Chemistry

“…A linear response was observed from 2.0 to 6.0 mg l -1 of melamine with a CV of 2.9%. In other SIC application, a green analyte extraction (water instead of organic solvents) and a significant reduction of solvent consumption in chromatographic separation (up to 100-fold lower consumption of acetonitrile) were combined for determination of the phytohormone salicylic acid in plant materials (Barrientos et al 2016).…”