Fast and highly efficient purification of 6×histidine-tagged recombinant proteins by Ni-decorated MnFe₂O₄@SiO₂@NH₂@2AB as novel and efficient affinity adsorbent magnetic nanoparticles

Abstract

A fast, convenient, and inexpensive method for the preparation of MnFe2O4@SiO2@NH2@2AB-Ni magnetic nanoparticles as an efficient and novel affinity adsorbent for the highly specific capture of 6×histidine-tagged recombinant protein-A is reported.

Preparation, characterization and catalytic application of nano-Fe3O4@SiO2@(CH2)3OCO2Na as a novel basic magnetic nanocatalyst for the synthesis of new pyranocoumarin derivatives

“…1 presents the X-ray diffraction (XRD) patterns of the magnetic nanocatalyst 1. As can be seen, six 25 The characteristic peaks conrming the presence of -OCO 2 Na is appeared around 10, 34, 36 and 43 (2q) which some of them is overlapped by the Fe 3 O 4 peaks. However, the appearance of a peak at 10.53 conrms the connection of -OCO 2 Na in nanocatalyst.…”

Sensitive detection of proteins in polyacrylamide gel via isatoic anhydride derivatization: Introduction of a low‐cost fluorescent prelabeling procedure

“…The relative mobility and band height, as a measure of band broadening, for labeled and unlabeled proteins were calculated and presented in Table . It comes from the small size of anthranilate tag and probable positive charge of anthranilate amine group under electrophoresis conditions that compensate positive charge of lysine amine group . Besides, introduction of a hydrophobic moiety into protein sequence is followed by change in SDS–protein complexion, which results in higher migration velocity of modified proteins .…”

One‐pot synthesis of 1,4‐dihydropyridines and N‐arylquinolines in the presence of copper complex stabilized on MnFe2O4 (MFO) as a novel organic–inorganic hybrid material and magnetically retrievable catalyst

“…Magnetic manganese ferrite nanoparticles (MnFe 2 O 4 ) were prepared using the co-precipitation method. [43] To a mixture of MnCl 2 , 4H 2 O (0.5 g, 2.5 mmol) and FeCl 3 , 6H 2 O (1.35 g, 5 mmol) in a round-bottom flask (100 ml), 50 ml deionized water was added and the solution was stirred for 1 h at 95 C. Subsequently, 10 ml of NH 3 was added and the brown mixture stirred for a further 1 h under the same conditions. The magnetic precipitate was isolated by an external magnet and washed with warm distilled water (200 ml) and EtOH (10 ml) and then dried at 70 C.…”