Fast and Reliable Determination of Voriconazole in Human Plasma by LC-APCI-MS/MS

Practice guidelines for therapeutic drug monitoring of voriconazole: a consensus review of the Japanese Society of Chemotherapy and the Japanese Society of Therapeutic Drug Monitoring

“…The various methods employed include HPLC with ultraviolet or mass spectrometric and bioassays; however, for the treatment of invasive antifungal infection, bioassays lack sensitivity and specificity compared with HPLC, when combination therapy is being used. Thus, HPLC may be the most suitable assay for measuring VRCZ concentrations [64–83]. …”

UPLC‐MS/MS determination of voriconazole in human plasma and its application to a pharmacokinetic study

“…Compared with chromatogram of blank blood sample, no interference of endogenous peaks was observed. The total run time was 1.0 min per sample, which was lower than previously reported analysis times (longer than 1.5 min; Pauwels et al ., ; Xiong et al ., ). This short analysis time indicated that this method better met the requirement of high sample throughput in bioanalysis.…”

“…However, the published analysis times in the literature vary between 3 and 13 min (Alffenaar et al ., ; Cheng et al ., ; Farowski et al ., ; Lin et al ., ; Xiong et al ., ). To our knowledge, only one method with a run time of 1.5 min has been described (Xiong et al ., ). This method, however, necessitates a time‐consuming solvent evaporation step.…”

Determination of Voriconazole Concentrations in Serum by GC‐MS

“…Several reports have shown different methods for measuring voriconazole from patient samples , relying on HPLC or LC‐MS/MS. However, presently LC‐MS/MS instruments have a limited presence in clinical laboratories due to a number of factors, including high initial cost, the complexity of operation, and limited expertise to operate and maintain them.…”