Fast and sample cleanup-free measurement of nicotine and cotinine by stable isotope dilution ultra-performance liquid chromatography–tandem mass spectrometry

Kühn, Joachim; Vollmer, Tanja; Martín, Claudia Fernández; Hendig, Doris; Knabbe, Cornelius

doi:10.1016/j.jpba.2012.04.036

Cited by 16 publications

(16 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, cross-reactivity is still the major concern for immunological methods, especially with nicotine metabolites: 3′-hydroxycotinine and 3′-hydroxycotinine-glucuronide [27]. The most commonly used analytical techniques are gas chromatography with flame-ionization detection (GC-FID, LOQ = 500 ng/mL) [28], mass spectrometry (GC-MS, LOQ = 10 ng/mL) [29], and liquid chromatography with mass spectrometry (UPLC-MS/MS, LOQ = 1.1 ng/mL) [30, 31]. These methods have been used to distinguish smokers from nonsmokers.…”

Section: Discussionmentioning

confidence: 99%

Estimation of Cutoff Values of Cotinine in Urine and Saliva for Pregnant Women in Poland

Stragierowicz

Mikołajewska

Zawadzka-Stolarz

et al. 2013

BioMed Research International

View full text Add to dashboard Cite

Setting appropriate cutoff values and the use of a highly sensitive analytical method allow for correct classification of the smoking status. Urine-saliva pairs samples of pregnant women in the second and third trimester, and saliva only in the first trimester were collected. Offline SPE and LC-ESI-MS/MS method was developed in the broad concentration range (saliva 0.4–1000 ng/mL, urine 0.8–4000 ng/mL). The mean recoveries were 3.7 ± 7.6% for urine and 99.1 ± 2.6% for saliva. LOD for saliva was 0.12 ng/mL and for urine 0.05 ng/mL; LOQ was 0.4 ng/mL and 0.8 ng/mL, respectively. Intraday and interday precision equaled, respectively, 1.2% and 3.4% for urine, and 2.3% and 6.4% for saliva. There was a strong correlation between salivary cotinine and the uncorrected cotinine concentration in urine in the second and third trimesters of pregnancy. The cutoff values were established for saliva 12.9 ng/mL and urine 42.3 ng/mL or 53.1 μg/g creatinine with the ROC curve analysis. The developed analytical method was successfully applied to quantify cotinine, and a significant correlation between the urinary and salivary cotinine levels was found. The presented cut-off values for salivary and urinary cotinine ensure a categorization of the smoking status among pregnant women that is more accurate than self-reporting.

show abstract

Section: Discussionmentioning

confidence: 99%

Estimation of Cutoff Values of Cotinine in Urine and Saliva for Pregnant Women in Poland

Stragierowicz

Mikołajewska

Zawadzka-Stolarz

et al. 2013

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…The most common methods include thin layer chromatography [3], capillary electrophoresis [4][5][6], gas chromatography [7][8][9][10], and high performance liquid chromatography (HPLC) [11][12][13][14][15][16][17]. Each of these methods has its advantages.…”

Section: Introductionmentioning

confidence: 99%

“…Each of these methods has its advantages. However, HPLC with its various separation modes can have the capability of being more specific and sensitive, especially if interfaced with tandem mass spectrometry (MS/MS) [13][14][15][16][17]. Nicotine related alkaloids belong to the group of very polar basic molecules, which in neutral and acidic solutions exist predominantly in the cationic form [4].…”

Section: Introductionmentioning

confidence: 99%

“…Nicotine related alkaloids belong to the group of very polar basic molecules, which in neutral and acidic solutions exist predominantly in the cationic form [4]. For this reason, HPLC separation of nicotine alkaloids can be carried out by ion pair [12], or ion chromatography [16], reversed-phase (RP) chromatography being the most common separation mode [11,[13][14][15]17].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of nicotine and three minor alkaloids in tobacco by hydrophilic interaction chromatography-tandem mass spectrometry

Taujenis¹,

Olšauskaitė²,

Padarauskas³

2015

Acta Chromatographica

View full text Add to dashboard Cite

Summary.A reliable, sensitive and rapid method for determination of nicotine and three minor alkaloids (cotinine, anabasine and nornicotine) in tobacco by ultra-high performance liquid chromatography in hydrophilic interaction chromatography mode coupled with tandem mass spectrometry (HILIC-MS/MS) has been established. HILIC separation was performed on a BEH HILIC column using isocratic elution at 0.5 mL/min with acetonitrile:water (85:15, v/v) mobile phase containing 5 mmol/L ammonium acetate (pH 5.00). Separated analytes were determined by electrospray ionization MS/MS in the positive ion mode using multiple reaction monitoring. Alkaloids from tobacco were extracted in an ultrasonic bath for 10 min with acetonitrile:water mixture (8:2, v/v) containing 5 mmol/L ammonium acetate (pH 5.00). Limits of quantification were 10 µg/g for cotinine, 20 µg/g for anabasine and nornicotine, and 30 µg/g for nicotine. Mean recoveries from tobacco ranged between 94.8% and 104.1% for different analytes with relative standard deviations within 5%. The performance of the proposed method was tested for the extraction and determination of the four alkaloids in cigarette tobacco fillers, and satisfactory results were achieved.

show abstract

“…Using this method, 83% recoveries of nicotine, cotinine and related compounds were obtained in urine and saliva samples with RSD <7.1%. Recently, Knabbe’s group developed a stable isotope dilution UPLC method coupled with ESI-MS/MS for determination of NIC, COT, the major oxidative and pharmacologically less active metabolite of NIC in human urine [82]. In this method, nicotine-d4 and cotinine-d4 were used as internal standards in sample preparation and the measurement of NIC and COT was performed within 1.5-min run-time.…”

Section: Determination Of Neurochemicals By Lc-ms Related Techniquesmentioning

confidence: 99%

Recent Advances in Mass Spectrometry for the Identification of Neurochemicals and their Metabolites in Biofluids

Kailasa

2013

View full text Add to dashboard Cite

Recently, mass spectrometric related techniques have been widely applied for the identification and quantification of neurochemicals and their metabolites in biofluids. This article presents an overview of mass spectrometric techniques applied in the detection of neurological substances and their metabolites from biological samples. In addition, the advances of chromatographic methods (LC, GC and CE) coupled with mass spectrometric techniques for analysis of neurochemicals in pharmaceutical and biological samples are also discussed.

show abstract

Fast and sample cleanup-free measurement of nicotine and cotinine by stable isotope dilution ultra-performance liquid chromatography–tandem mass spectrometry

Cited by 16 publications

References 32 publications

Estimation of Cutoff Values of Cotinine in Urine and Saliva for Pregnant Women in Poland

Estimation of Cutoff Values of Cotinine in Urine and Saliva for Pregnant Women in Poland

Determination of nicotine and three minor alkaloids in tobacco by hydrophilic interaction chromatography-tandem mass spectrometry

Recent Advances in Mass Spectrometry for the Identification of Neurochemicals and their Metabolites in Biofluids

Contact Info

Product

Resources

About