Fast and sensitive detection of foodborne pathogen using electrochemical impedance analysis, urease catalysis and microfluidics

Chen, Qi; Wang, Dan; Cai, Gaozhe; Xiong, Yonghua; Li, Yuntao; Wang, Maohua; Huo, Huiling; Lin, Jianhan

doi:10.1016/j.bios.2016.07.071

Cited by 96 publications

(45 citation statements)

References 18 publications

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“…and Lim, 2002;Ko and Grant, 2006;Liu et al, 2003;Ohk and Bhunia, 2012;Sharma and Mutharasan, 2013). At present, the focus is shifting towards the integration of SPR into microfluidic systems to establish versatile lab-on-fibre technology for the POC applications (Blue and Uttamchandani, 2016;Chen et al, 2016;Lin et al, 2015;Luka et al, 2015;Oscar et al, 2017;Ricciardi et al, 2015;Sun et al, 2016;M. Yin et al, 2016).…”

Section: Optical Fibre Biosensorsmentioning

confidence: 99%

“…This microfluidic biosensor showed a 10-fold increase in sensitivity compared to a non-microfluidic biosensor (Ghosh Dastider et al, 2015). Online bacterial separation and detection is also achieved by integrating electrochemical impedance analysis and urease catalysis into a microfluidic device (Chen et al, 2016). For this purpose, Listeria monocytogenes cells, magnetic nanoparticles modified with the anti-Listeria monoclonal antibodies, and gold nanoparticles modified with anti-Listeria polyclonal antibodies and urease, were all mixed and incubated in a fluidic separation chip to form sandwich complexes of magnetic nanoparticle-Listeria-urease conjugated gold nanoparticles.…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

“…Gold nanoparticles, iron oxide magnetic nanoparticles, and carbon-based nanomaterials have provided promising opportunities to explore nanoscale electrochemical biosensors due to their biocompatibility and high surface areas that can enhance bio-receptor loading and stability. These conductive materials can serve as efficient agents for electron transfer between the bio-receptors and the electrode surface, allowing electrochemical sensing of foodborne pathogens without necessity of using electron transfer mediators (Wang, 2005;Hernandez and Ozalp, 2012;Wang and Dai, 2015;Zeng et al, 2016;Chen et al, 2016). Ma et al (2014) proposed an impedimetric biosensor using graphene oxide and gold nanoparticle modified glassy carbon electrodes as transducers for Salmonella spp.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Microfluidic devices for sample preparation and rapid detection of foodborne pathogens

Kant

Shahbazi

Dave

et al. 2018

Biotechnology Advances

152

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Rapid detection of foodborne pathogens at an early stage is imperative for preventing the outbreak of foodborne diseases, known as serious threats to human health. Conventional bacterial culturing methods for foodborne pathogen detection are time consuming, laborious, and with poor pathogen diagnosis competences. This has prompted researchers to call the current status of detection approaches into question and leverage new technologies for superior pathogen sensing outcomes. Novel strategies mainly rely on incorporating all the steps from sample preparation to detection in miniaturized devices for online monitoring of pathogens with high accuracy and sensitivity in a time-saving and cost effective manner. Lab on chip is a blooming area in diagnosis, which exploits different mechanical and biological techniques to detect very low concentrations of pathogens in food samples. This is achieved through streamlining the sample handling and concentrating procedures, which will subsequently reduce human errors and enhance the accuracy of the sensing methods. Integration of sample preparation techniques into these devices can effectively minimize the impact of complex food matrix on pathogen diagnosis and improve the limit of detections. Integration of pathogen capturing bio-receptors on microfluidic devices is a crucial step, which can facilitate recognition abilities in harsh chemical and physical conditions, offering a great commercial benefit to the food-manufacturing sector. This article reviews recent advances in current state-of-the-art of sample preparation and concentration from food matrices with focus on bacterial capturing methods and sensing technologies, along with their advantages and limitations when integrated into microfluidic devices for online rapid detection of pathogens in foods and food production line.

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Section: Optical Fibre Biosensorsmentioning

confidence: 99%

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Section: Accepted Manuscriptmentioning

confidence: 99%

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Microfluidic devices for sample preparation and rapid detection of foodborne pathogens

Kant

Shahbazi

Dave

et al. 2018

Biotechnology Advances

152

View full text Add to dashboard Cite

show abstract

“…The anti-E. coli polyclonal antibodies (PAbs) were immobilized on the surface of the MNPs for specific separation of the E. coli cells based on previously-reported protocol with small modifications [18] .…”

Section: Preparation Of the Magnetic Nanoparticlesmentioning

confidence: 99%

Development of automatic and efficient immuno-separator of foodborne pathogenic bacteria using magnetophoresis and magnetic mixing

Wang

Cai

Wang

et al. 2019

International Journal of Agricultural and Biological Engineering

Self Cite

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In recent years, the outbreaks of foodborne diseases caused by pathogenic bacteria have made considerable economic losses and shown a threat to public health. The key to prevent and control these diseases is fast screening of pathogenic bacteria, which is usually performed with three procedures: sample collection, bacteria separation and bacteria detection. For sample collection, the national standard methods are often employed. For bacteria detection, currently available methods such as Polymerase Chain Reaction and Enzyme Linked Immuno-Sorbent Assay are often used. For bacteria separation, traditional methods such as filtration and centrifugation are not capable to specifically separate the target bacteria. However, food samples are very complicated and require efficient pretreatment for bacteria separation and concentration to achieve accurate and reliable results. The conventional immune magnetic separation method can be used to specifically separate the bacteria, but it still cannot meet the requirements for food sample pretreatment due to very low concentration of target bacteria in food. Therefore, this study developed an automatic and efficient immuno-separator of foodborne bacteria based on magnetophoresis and magnetic mixing, and E. coli O157:H7 was used as research model. A magnetic mixer was applied to facilitate the immunoreaction between the immune magnetic nanoparticles and the target bacteria cells, and a magnetophoretic separation tubing was utilized for magnetophoretic separation of the magnetic bacteria. Under the optimal mixing time of 20 min and the optimal flow rate of 50 µL/min, the separation efficiency of E. coli O157:H7 could be more than 90%, showing that the developed immuno-separator is promising to be applied for efficient separation of foodborne bacteria and can be easily extended for separation of other biological targets by using their specific antibodies.

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“…C11E9 and E-cadherin), Hsp60 could detect pathogenic Listeria with high specificity. A newer approach for the detection of Listeria species was developed by Chen et al (2016) integrating electrochemical impedance with urease catalysis with the microfluidics in spiked lettuce sample. A LOD of 1.6×10 2 cfu/mL in ∼1 h was obtained using this method.…”

Section: Impedimetric Based Microfluidic Biosensorsmentioning

confidence: 99%

Biosensor for the detection of Listeria monocytogenes: emerging trends

Soni

Ahmad

Dubey

2018

Critical Reviews in Microbiology

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The early detection of Listeria monocytogenes (L. monocytogenes) and understanding the disease burden is of paramount interest. The failure to detect pathogenic bacteria in the food industry may have terrible consequences, and poses deleterious effects on human health. Therefore, integration of methods to detect and trace the route of pathogens along the entire food supply network might facilitate elucidation of the main contamination sources. Recent research interest has been oriented towards the development of rapid and affordable pathogen detection tools/techniques. An innovative and new approach like biosensors has been quite promising in revealing the foodborne pathogens. In spite of the existing knowledge, advanced research is still needed to substantiate the expeditious nature and sensitivity of biosensors for rapid and in situ analysis of foodborne pathogens. This review summarizes recent developments in optical, piezoelectric, cell-based, and electrochemical biosensors for Listeria sp. detection in clinical diagnostics, food analysis, and environmental monitoring, and also lists their drawbacks and advantages.

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Fast and sensitive detection of foodborne pathogen using electrochemical impedance analysis, urease catalysis and microfluidics

Cited by 96 publications

References 18 publications

Microfluidic devices for sample preparation and rapid detection of foodborne pathogens

Microfluidic devices for sample preparation and rapid detection of foodborne pathogens

Development of automatic and efficient immuno-separator of foodborne pathogenic bacteria using magnetophoresis and magnetic mixing

Biosensor for the detection of Listeria monocytogenes: emerging trends

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