2020
DOI: 10.1038/s41467-020-17026-6
|View full text |Cite
|
Sign up to set email alerts
|

Fast and sensitive flow-injection mass spectrometry metabolomics by analyzing sample-specific ion distributions

Abstract: Mass spectrometry based metabolomics is a widely used approach in biomedical research. However, current methods coupling mass spectrometry with chromatography are timeconsuming and not suitable for high-throughput analysis of thousands of samples. An alternative approach is flow-injection mass spectrometry (FI-MS) in which samples are directly injected to the ionization source. Here, we show that the sensitivity of Orbitrap FI-MS metabolomics methods is limited by ion competition effect. We describe an approac… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
60
0
1

Year Published

2020
2020
2023
2023

Publication Types

Select...
5
1

Relationship

0
6

Authors

Journals

citations
Cited by 61 publications
(64 citation statements)
references
References 40 publications
0
60
0
1
Order By: Relevance
“…Multiple MS acquisition methods with different scan ranges were used, including i) full scan in range from 50 to 500 m/z ; (ii) targeted with the narrowest scan window possible from 204.8 to 205.2 m/z ; iii) twenty-two 20 m/z windows with an overlap of 4 m/z ; iv) six windows covering the 50 to 500 m/z range splitted either according to the equal-feature number criterion (equal number of robust features per window -the same strategy as in [13]) or the equal-cumulative intensity criterion (equal accumulated intensity per window), with an overlap of 1 m/z. Figure 1(a) shows how these ranges are allocated while a subject is exhaling.…”
Section: Real-time Sesi-hrms Measurements Of Breath and Bacteria Cultmentioning
confidence: 99%
See 4 more Smart Citations
“…Multiple MS acquisition methods with different scan ranges were used, including i) full scan in range from 50 to 500 m/z ; (ii) targeted with the narrowest scan window possible from 204.8 to 205.2 m/z ; iii) twenty-two 20 m/z windows with an overlap of 4 m/z ; iv) six windows covering the 50 to 500 m/z range splitted either according to the equal-feature number criterion (equal number of robust features per window -the same strategy as in [13]) or the equal-cumulative intensity criterion (equal accumulated intensity per window), with an overlap of 1 m/z. Figure 1(a) shows how these ranges are allocated while a subject is exhaling.…”
Section: Real-time Sesi-hrms Measurements Of Breath and Bacteria Cultmentioning
confidence: 99%
“…Two approaches have been considered here to optimize the window splitting of the mass range. First, we split the complete mass range into windows containing an equal number of robust features as suggested by Sarvin et al [13] Second, we split such that the windows contain an equal cumulative intensity of the robust features per window.…”
Section: Optimized Mass Window For Human Breath and Bacteria Head Spamentioning
confidence: 99%
See 3 more Smart Citations