Fast and sensitive LC‐MS/MS method for quantification of cannabinoids and their metabolites in plasma of cattle fed hemp

Abstract

Hemp‐based materials have gained interest as alternative feed ingredients for livestock. However, safety concerns arise regarding the transfer of cannabinoids from the plant to the animals. Addressing these concerns requires the use of methods capable of detecting and quantifying cannabinoids in livestock. In this study, a fast and sensitive method was developed for quantification of cannabinoids and cannabinoid metabolites in cattle plasma using liquid chromatography‐tandem mass spectrometry (LC‐MS/MS). The extraction of cannabinoids from the plasma matrix was achieved by combining the Captiva Enhanced Matrix Removal‐Lipid clean‐up and salting‐out assisted liquid–liquid extraction procedure. The developed method underwent validation using various analytical parameters, and the results demonstrated good accuracy, precision, specificity, and high sensitivity. The method was applied to real plasma samples obtained from cattle fed hemp for 2 weeks, and successfully detected various cannabinoids, including delta‐9‐tetrahydrocannabinol. Furthermore, the study revealed that 7‐carboxy cannabidiol, a metabolite of cannabidiol, was the predominant cannabinoid present in the cattle plasma throughout the feeding period, which could remain detectable for weeks after the hemp feeding had ended.

Mapping and Screening of Candidate Gene Regulating the Biomass Yield of Sorghum (Sorghum bicolor L.)

“…Each experiment contained at least two biological replicates. The detection of tryptamine, melatonin and auxin was conducted at MetWare (http://www.metware.cn/, accessed on 5 May 2021) based on the AB Sciex QTRAP 6500 LC-MS/MS platform, which ensures accurate and reliable measurements [60]. The components were extracted according to the method described in a previous study [61].…”