1991
DOI: 10.1016/0003-2697(91)90120-i
|View full text |Cite
|
Sign up to set email alerts
|

Fast and sensitive silver staining of DNA in polyacrylamide gels

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

4
1,311
1
31

Year Published

1997
1997
2008
2008

Publication Types

Select...
10

Relationship

0
10

Authors

Journals

citations
Cited by 2,514 publications
(1,347 citation statements)
references
References 12 publications
4
1,311
1
31
Order By: Relevance
“…30 A DNA ladder with fragments at 100 bases pairs (100-1000) was used in this assay. Scanning of gels was used for determination of band localization and band intensity in each lane using the Image J program.…”
Section: Measurement Of Dna Fragmentationmentioning
confidence: 99%
“…30 A DNA ladder with fragments at 100 bases pairs (100-1000) was used in this assay. Scanning of gels was used for determination of band localization and band intensity in each lane using the Image J program.…”
Section: Measurement Of Dna Fragmentationmentioning
confidence: 99%
“…Several variables were tested in order to achieve an optimum separation of the alleles: acrylamide concentrations (from 6 to 16%), acrylamide bis ratio (19:1, 29:1, 100:1), glycerol level (0%, 5% or 10%), electrophoresis temperature (from 6 to 26 • C) and buffer conditions (0.5X or 0.6X). The bands were visualised by silver-staining according to the method of Bassam et al [2] with minor modifications [3]. Silver-stained allele-specific SSCP bands were excised from the gel, placed in 100 µL of distilled water and subjected to 95 • C for 15 min, two freezing (−70 • C) and thawing cycles and then centrifuged at 10 000 × g for 2 min.…”
Section: Sscpmentioning
confidence: 99%
“…Primers and Polymerase Chain Reaction (PCR) conditions are described in Table II. The PCR analysis of microsatellites was carried out by loading onto standard 7% polyacrilamide denaturing gel using silver staining [2] or fluorescent-labelled PCR primer methods through an automated DNA fragment analyser (Applied Biosystem 373 or 377). In order to ensure the compatibility of results from different equipment and laboratories, 3 types of reference DNA were used: Type 1 = reference DNAs (n = 9) from the AIRE 2006, Type 2 = reference DNA (n = 4) from this project, Type 3 = reference DNA (n = 2) from individual laboratories.…”
Section: Dna Extraction and Pcr Amplificationmentioning
confidence: 99%