Fast Degradation of Chlorpyrifos in Soil by Strain <i>Cupriavidus taiwanensis</i> X1 Labeled with LuxAB

Abstract

For researching ecological behaviors of Cupriavidus taiwanensis X1 which has strong hydrolysis activity on chlorpyrifos(CP), reporter gene luxAB was successfully introduced into cells by electroporation. The labeled strain X1-lux with genetic stability and fluorescence was obtained. Both of strain X1 and X1-lux could completely degrade 200 mg/l CP within 12h in minimal salt medium, and experimental results showed that introduction of luxAB did not affect strain X1 growth and degradation on CP. The cells of X1-lux and X1 were inoculated into soil with 500 mg/l CP, the cell concentration and CP residual was detected, and data revealed that strain X1 could absolutely removed CP in 12d and survive in soil. Strain X1 is a potential excellent choice for bioremediation of organophosphorus polluted environments.