Fast-degrading PLA/ORMOGLASS fibrous composite scaffold leads to a calcium-rich angiogenic environment

Abstract

The success of scaffold implantation in acellular tissue engineering approaches relies on the ability of the material to interact properly with the biological environment. This behavior mainly depends on the design of the graft surface and, more precisely, on its capacity to biodegrade in a well-defined manner (nature of ions released, surface-to-volume ratio, dissolution profile of this release, rate of material resorption, and preservation of mechanical properties). The assessment of the biological behavior of temporary templates is therefore very important in tissue engineering, especially for composites, which usually exhibit complicated degradation behavior. Here, blended polylactic acid (PLA) calcium phosphate ORMOGLASS (organically modified glass) nanofibrous mats have been incubated up to 4 weeks in physiological simulated conditions, and their morphological, topographical, and chemical changes have been investigated. The results showed that a significant loss of inorganic phase occurred at the beginning of the immersion and the ORMOGLASS maintained a stable composition afterward throughout the degradation period. As a whole, the nanostructured scaffolds underwent fast and heterogeneous degradation. This study reveals that an angiogenic calcium-rich environment can be achieved through fast-degrading ORMOGLASS/PLA blended fibers, which seems to be an excellent alternative for guided bone regeneration.

In vitro evaluation of degradable electrospun polylactic acid/bioactive calcium phosphate ormoglass scaffolds

“…26] and region between 480 and 600 cm −1 relating to the bending vibrations O-P-O and O=P-O in phosphate groups [25,27]. The intensity of those peaks increases for higher content of ormoglass filler which suggests they are related to the vibration of PO 4 phosphate groups in CaP ormoglass as shown in our previous work [28]. No significant differences can be observed between aligned and randomly oriented nonwovens.…”

Innovative biodegradable poly(L-lactide)/collagen/<br />hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation

“…Therefore, scaffold-based polymer such as polycaprolactone (PCL) or polylactic acid (PLLA) were investigated for bone tissue engineering in recent years. [13][14][15] In vitro studies showed that a membrane system-based PCL can promote proliferation and early cell differentiation of osteoblast-like cells. 15 However, the disadvantage of the synthetic polymers is that it has a poor biocompatibility.…”

Bioinorganic Angiogenesis

“…Indeed a pro-angiogenic effect of calcium ions has been reported [34,35] While not conclusive, these observations are suggestive of chemical effect. [36][37][38][39] The ability of the monetite to resorb, apparently due to dissolution (Figure S4), may have also simply allowed more and larger blood vessel ingrowth. Indeed, the degradation of a scaffold at a rate matching the development of a new tissue is often considered as an ideal requirement.…”