Fast Determination of Naringin and Hesperidin in Natural and Commercial Citrus Juices by HPLC Method

Büyüktuncel, Ebru

doi:10.14233/ajchem.2017.20675

Cited by 13 publications

(6 citation statements)

References 11 publications

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“…The peaks (Figure 6a,b) correspond to naringin have Retention time 10.812 min and peak of Naringenin has Retention time 12.289 min. Previous studies have also reported the quantification of naringin and naringenin from orange juice, grapefruit juice and tangerine juice has been done using rapid and reliable HPLC method (Buyuktuncel, 2017).…”

Section: Resultsmentioning

confidence: 99%

Enzymatic debittering of Citrus reticulata (Kinnow) pulp residue and its utilization for the preparation of vermicelli

Singla

Panesar

Sangwan

et al. 2020

J. Food Process. Preserv.

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This study involves the debittering of Citrus reticulata (kinnow) pulp residue and its value addition through incorporation for the preparation of vermicelli. The optimization of debittering has been monitored through conversion of naringin (bitterness causing compound) to naringenin (nonbitter compound) through enzymatic method. The enzyme (naringinase) concentration has been varied from 0.5 to 2.5 U/mg with temperature range of 25–65°C at pH 4.5 with incubation period of 2–6 hr. The maximum reduction (~66.19%) naringin was observed in the kinnow pulp residue, with increase in the naringenin concentration (nonbitter) of 52.38% under optimized conditions, that is, enzyme concentration (1 U/mg), temperature (50°C), and treatment time (4 hr) at natural pH (4.5). The debittered kinnow pulp residue was characterized for compositional analysis and morphological studies using SEM and FTIR. The obtained debittered kinnow pulp residue was further successfully utilized in the preparation of nutrient and antioxidant‐rich vermicelli. Practical applications Food waste has been generated in abundance by fruits processing industries which are rich in mineral, antioxidants, and polyphenols. The bitterness in citrus waste (kinnow pulp, peel, and seeds), has posed a major issue for their utilization in different food products due to the problem of consumer acceptability. In present investigation, reduction in the content of naringin below the threshold limit resulted in debittering of kinnow pulp residue. Furthermore, this debittered pulp residue has been explored for its utilization for the preparation of antioxidant rich vermicelli. Results of this work depicted that such technology can be used for debittering of kinnow industry by‐products and can be subsequently utilized by the food industry in the preparation of antioxidant rich food products. This process is not only an effective utilization of agro‐industrial by‐products/wastes but also solution for environmental waste caused by kinnow juice industry.

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Section: Resultsmentioning

confidence: 99%

Enzymatic debittering of Citrus reticulata (Kinnow) pulp residue and its utilization for the preparation of vermicelli

Singla

Panesar

Sangwan

et al. 2020

J. Food Process. Preserv.

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“…In this case, chromatography with different types of detectors is the most often applied method for its identification and determination with high selectivity and acceptable sensitivity. High-performance liquid chromatography (HPLC) with UV- [5][6][7][8], diode-array [9,10] and tandem massspectrometric [11][12][13] detection is usually applied for these purposes. Recently, several approaches based on ultra-HPLC with tandem mass-spectrometric detector [14,15] and high-performance thin-layer chromatography with UV- [16] and densitometric [17] detection have been developed.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Determination of Hesperidin on the Electrode Modified with SnO₂ Nanoparticles and Surfactants

2021

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Highly sensitive voltammetric method for hesperidin determination is developed using glassy carbon electrode modified with SnO 2 nanoparticles (SnO 2 NPs) and surfactants. The highest oxidation currents of hesperidin are observed in the case of cationic 0.50 mM cetylpyridinium bromide (CPB). Parameters of hesperidin electrooxidation have been calculated. Adsorptive anodic differential pulse voltammetry (AdADPV) in phosphate buffer pH 7.0 after 120 s accumulation provides linear dynamic ranges of 0.10-10 and 10-75 μM with the detection limit of 0.077 μM. The selectivity of hesperidin response is proved in the presence of inorganic ions, saccharides, ascorbic acid and other natural phenolics.

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“…Briefly, NR and p(NR) particles were placed in 10 mL of methanol at 1 mg/mL concentration at room temperature at 300 rpm mixing rate for 1 h. Then, these solutions were filtered through an 0.45 μm syringe filter and analyzed with HPLC at 1 mL/min flow rate with 15 min run time at 40 °C column temperature employing 20 μL of injection volumes. The mobile phase was 230:768:2 mL acetonitrile/water/formic acid, in accordance with the literature …”

Section: Methodsmentioning

confidence: 99%

“…The mobile phase was 230:768:2 mL acetonitrile/water/formic acid, in accordance with the literature. 22 The structural characterizations of NR and p(NR) particles included solid-state NMR spectroscopy and FT-IR spectroscopy. NMR experiments employed a CP/MAS 13 C NMR method (JEOL ECZ500R, 11.75 T) at 6 kHz spinning rate.…”

Section: ■ Introductionmentioning

confidence: 99%

Fabrication of Biodegradable Poly(naringin) Particles with Antioxidant Activity and Low Toxicity

Şahiner

Sagbas

et al. 2018

ACS Omega

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Naringin (NR, 4′,5,7 trihydrocyflavanone-7-O-rhamnoglucoside) is a flavanone found in citrus fruit that is composed of a phenolic compound, naringenin, and a disaccharide, neohesperidose. Poly(NR) [p(NR)] particles in the size range of few micrometers to few hundred nanometers were prepared from highly purified NR and subsequently characterized using UV/visible spectroscopy, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, scanning electron microscopy, thermogravimetric analysis, and zeta potential measurements. The hydrolytic degradation of p(NR) particles at 37.5 °C was investigated at pHs 5.4, 7.4, and 9.0. The particles degraded most rapidly at pH 7.4, with >90% degradation after 5 h. The cytotoxicity was assessed by growing COS-1 fibroblasts for 5 days in the presence of increasing concentrations of NR or eluates from the p(NR) particles. Both NR and p(NR) particles were nontoxic for these mammalian fibroblasts; at the highest concentration tested (571 μg/mL), the percentages of surviving cells after 5 days in culture were 96.7 ± 0.93 and 91 ± 11% for NR and p(NR), respectively. The effect of p(NR) on red blood cell hemolysis was also negligible (<2%) at concentrations up to 100 μg/mL; unpolymerized NR showed slightly higher levels of hemolysis than the p(NR) particles, but values never exceeded 5% at 50 and 100 μg/mL. Both NR and p(NR) stimulated blood coagulation in a dose-dependent manner; the hemostatic effect was greater for p(NR), which, at 100 μg/mL, stimulated in vitro blood clotting by ∼50%. Neither NR nor eluates from p(NR) particles inhibited α-glucosidase activity; in fact, both provided a modest (10–30%) stimulation at concentrations from 0.67 to 1.67 mg/mL. p(NR) particles are easily synthesized, break down readily at physiological pH, and have excellent blood and biocompatibility. As such, they will be a useful carrier for drug delivery and as an oral antioxidant supplement.

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Fast Determination of Naringin and Hesperidin in Natural and Commercial Citrus Juices by HPLC Method

Cited by 13 publications

References 11 publications

Enzymatic debittering of Citrus reticulata (Kinnow) pulp residue and its utilization for the preparation of vermicelli

Enzymatic debittering of Citrus reticulata (Kinnow) pulp residue and its utilization for the preparation of vermicelli

Voltammetric Determination of Hesperidin on the Electrode Modified with SnO₂ Nanoparticles and Surfactants

Fabrication of Biodegradable Poly(naringin) Particles with Antioxidant Activity and Low Toxicity

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Fast Determination of Naringin and Hesperidin in Natural and Commercial Citrus Juices by HPLC Method

Cited by 13 publications

References 11 publications

Enzymatic debittering of Citrus reticulata (Kinnow) pulp residue and its utilization for the preparation of vermicelli

Enzymatic debittering of Citrus reticulata (Kinnow) pulp residue and its utilization for the preparation of vermicelli

Voltammetric Determination of Hesperidin on the Electrode Modified with SnO2 Nanoparticles and Surfactants

Fabrication of Biodegradable Poly(naringin) Particles with Antioxidant Activity and Low Toxicity

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Voltammetric Determination of Hesperidin on the Electrode Modified with SnO₂ Nanoparticles and Surfactants