Fast emerging insecticide resistance in Aedes albopictus in Guangzhou, China: Alarm to the dengue epidemic

Su, Xinghua; Guo, Yijia; Deng, Jielin; Xu, Jiabao; Zhou, Guofa; Zhou, Tengfei; Li, Yiji; Zhong, Daibin; Kong, Ling; Wang, Xiaoming; Liu, Min; Wu, Kun; Yan, Guiyun; Chen, Xiaoguang

doi:10.1371/journal.pntd.0007665

Cited by 64 publications

(95 citation statements)

References 34 publications

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“…albopictus and Ae. aegypti populations [38][39][40]. Overall, this study demonstrated the feasibility of using the PPF dissemination strategy as a complementary method to reduce cryptic sources of dengue vectors after source reduction in communities of Southern Taiwan, with the benefits of easy application, low ecotoxicity, and lack of resistance.…”

Section: Discussionmentioning

confidence: 88%

Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan

Chen

Lai

et al. 2020

Insects

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The policy regarding mosquito control strategies in Taiwan is based on integrated vector management (IVM). The major approach is source reduction via collaboration by both residents and governments. However, small and cryptic habitats of dengue vectors are hard to find and eliminate in urban communities. Therefore, this study evaluated a complementary approach that targeted cryptic habitats by utilizing mosquitoes themselves as vehicles to transfer an insect growth regulator, pyriproxyfen (PPF), to their breeding sites; the amount of PPF in breeding water was determined with ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). A bioassay conducted by introducing ten late-instar larvae into PPF solution was performed to assess emergence inhibition (EI). PPF was found at 0.56 ± 0.04 ng in 25 mL of water by dissemination via ten Aedes aegypti mosquitoes exposed to 0.01% PPF, leading to 100% EI. After the community-level source reduction, a field trial in Kaohsiung in Southern Taiwan showed that 30.8-31.5% of cryptic ovitraps reached EI ≥ 50% one month after spraying 0.01% PPF in microhabitats favored by mosquitoes. IVM in parallel with residual spraying of PPF on resting surfaces of mosquitoes could serve as a simple and complementary approach to reduce cryptic larval sources in urban communities in Southern Taiwan.

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Section: Discussionmentioning

confidence: 88%

Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan

Chen

Lai

et al. 2020

Insects

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“…Results were compared to the LC 50 and LC 95 values reported for susceptible-laboratory Ae. albopictus strains reported in other studies [36,37,38].…”

Section: Insecticide Susceptibility Bioassays A) Larval Bioassaysmentioning

confidence: 56%

“…albopictus laboratory strains in other studies. Indicatively, in Li et al, 2018 [37] and Su et al, 2019 [38] the reported LC 50 values of the control susceptible strains were 0.036 mg/L and 0.044 mg/L, respectively (both corresponding to more than 0.240 ITU/ mL).…”

Section: Insecticide Susceptibility Bioassays A) Larval Bioassaysmentioning

confidence: 99%

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Phenotypic and molecular analysis of insecticide resistance in Aedes albopictus populations from Greece

Balaska¹,

Fotakis²,

Kioulos³

et al. 2020

Preprint

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Abstract Background: Aedes albopictus has a well-established presence in southern European countries, associated with recent disease outbreaks (e.g. Chikungunya). Development of insecticide resistance in the vector is a major concern as it’s control mainly relies on the use of biocides. Data on the specie’s resistance status is essential for efficient and sustainable control. Methods: We investigated the insecticide resistance status of several Ae. albopictus populations from Greece. Bioassays were performed against diflubenzuron (DFB), B. thuringiensis var. israelensis (Bti), deltamethrin and malathion. Molecular analysis of known insecticide resistance loci was performed, i.e. voltage-gated sodium channel (VGSC) mutations associated with pyrethroid resistance; presence and frequency of carboxylesterases 3 (CCEae3a) and 6 (CCEae6a) gene amplification associated with organophosphate (OP) resistance and; chitin synthase-1 (CHS-1) for the possible presence of DFB resistance mutations. Results: Bioassays showed full susceptibility to DFB, Bti and deltamethrin, but resistance against the OP malathion. VGSC analysis revealed a widespread distribution of mutations F1534C (in all populations, with allelic frequencies between 6.6% - 68.3%), and I1532T (in 6 populations), but absence of V1016G. CCE gene amplifications were recorded in 8 out of 11 populations. Co-presence of mutation F1534C and CCEae3a amplification was reported in a subgroup of samples. No mutations at the CHS locus I1043 were detected. Conclusions: The results indicate: (i) the suitability of larvicides DFB and Bti for Ae. albopictus control in Greece, (ii) a possible incipient pyrethroid resistance due to the presence of kdr mutations and (iii) a possible reduced efficacy of OPs, in a scenario of re-introducing them for vector control. The study highlights the need for systematic resistance monitoring for developing and implementing appropriate evidence-based control programs. Key words: diagnostic, arbovirus, mosquito tiger, insecticide resistance, vector control, Europe

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“…The OPs malathion and temephos appear unreliable alternative insecticides for possible future re-introduction in mosquito control. Abbreviations: n: total number of specimens analyzed per sampling region for species identification Notes: Mortality percentages correspond to the discriminating exposure time DT=30 min for both insecticides [39]. The average values of four insecticide treated replicate bottles are presented.…”

Section: Discussionmentioning

confidence: 99%

Bioassay and molecular monitoring of insecticide resistance status in Aedes albopictus populations from Greece, to support evidence-based vector control

Balaska

Fotakis

Kioulos

et al. 2020

Preprint

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Abstract Background: Aedes albopictus has a well-established presence in southern European countries, associated with recent disease outbreaks (e.g. chikungunya). Development of insecticide resistance in the vector is a major concern as its control mainly relies on the use of biocides. Data on the species’ resistance status are essential for efficient and sustainable control. To date the insecticide resistance status of Ae. albopictus populations from Greece against major insecticides used in vector control remains largely unknown. Methods: We investigated the insecticide resistance status of 19 Ae. albopictus populations from 11 regions of Greece. Bioassays were performed against diflubenzuron (DFB), Bacillus thuringiensis var. israelensis (Bti), deltamethrin and malathion. Known insecticide resistance loci were molecularly analysed, i.e. voltage-gated sodium channel (VGSC) mutations associated with pyrethroid resistance; presence and frequency of carboxylesterases 3 (CCEae3a) and 6 (CCEae6a) gene amplification associated with organophosphate (OP) resistance and; chitin synthase-1 (CHS-1) for the possible presence of DFB resistance mutations. Results: Bioassays showed full susceptibility to DFB, Bti and deltamethrin, but resistance against the OP malathion (range of mortality: 55.30–91.40%). VGSC analysis revealed a widespread distribution of the mutations F1534C (in all populations, with allelic frequencies between 6.6–68.3%), and I1532T (in 6 populations; allelic frequencies below 22.70%), but absence of V1016G. CCE gene amplifications were recorded in 8 out of 11 populations (overall frequency: 33%). Co-presence of the F1534C mutation and CCEae3a amplification was reported in 39 of the 156 samples analysed by both assays. No mutations at the CHS-1 I1043 locus were detected. Conclusions: The results indicate: (i) the suitability of larvicides DFB and Bti for Ae. albopictus control in Greece; (ii) possible incipient pyrethroid resistance due to the presence of kdr mutations; and (iii) possible reduced efficacy of OPs, in a scenario of re-introducing them for vector control. The study highlights the need for systematic resistance monitoring for developing and implementing appropriate evidence-based control programmes.

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Fast emerging insecticide resistance in Aedes albopictus in Guangzhou, China: Alarm to the dengue epidemic

Cited by 64 publications

References 34 publications

Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan

Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan

Phenotypic and molecular analysis of insecticide resistance in Aedes albopictus populations from Greece

Bioassay and molecular monitoring of insecticide resistance status in Aedes albopictus populations from Greece, to support evidence-based vector control

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