Fast flip-flop of cholesterol and fatty acids in membranes: implications for membrane transport proteins

Ham,

doi:10.1097/01.mol.0000073507.41685.9b

Cited by 2 publications

(2 citation statements)

References 27 publications

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“…Serum typically contains external lipids in two forms: non-esterified free fatty acids (FFAs) associated with albumin, and lipoproteins that carry triglycerides, cholesterol and phospholipids encapsulated by apoproteins [8, 9]. Cells can take up FFAs via physical diffusion across the cellular membrane [10] or via active transport aided by membrane-associated protein CD36 or FATPs [11]. Lipoprotein uptake occurs through binding to cell surface receptors (e.g.…”

Section: Introductionmentioning

confidence: 99%

A lipid-free and insulin-supplemented medium supports De Novo fatty acid synthesis gene activation in melanoma cells

Näär

2019

PLoS ONE

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While investigating the role played by de novo lipid (DNL) biosynthesis in cancer cells, we sought a medium condition that would support cell proliferation without providing any serum lipids. Here we report that a defined serum free cell culture medium condition containing insulin, transferrin and selenium (ITS) supports controlled study of transcriptional regulation of de novo fatty acid (DNFA) production and de novo cholesterol synthesis (DNCS) in melanoma cell lines. This lipid-free ITS medium is able to support continuous proliferation of several melanoma cell lines that utilize DNL to support their lipid requirements. We show that the ITS medium stimulates gene transcription in support of both DNFA and DNCS, specifically mediated by SREBP1/2 in melanoma cells. We further found that the ITS medium promoted SREBP1 nuclear localization and occupancy on DNFA gene promoters. Our data show clear utility of this serum and lipid-free medium for melanoma cancer cell culture and lipid-related areas of investigation.

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Section: Introductionmentioning

confidence: 99%

A lipid-free and insulin-supplemented medium supports De Novo fatty acid synthesis gene activation in melanoma cells

Näär

2019

PLoS ONE

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“…In the past, it has been often suggested that cholesterol flip-flop, i.e., the rapid exchange of cholesterol between the two leaflets, can significantly contribute to domain registration (Hamilton, 2003). The proposed mechanism would result primarily from the fact that cholesterol can move significantly faster in the disordered phase in comparison to the ordered one (Risselada and Marrink, 2008).…”

Section: Cholesterolmentioning

confidence: 99%

Interleaflet Coupling of Lipid Nanodomains – Insights From in vitro Systems

Sarmento

Hof

Šachl

2020

Front. Cell Dev. Biol.

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The plasma membrane is a complex system, consisting of two layers of lipids and proteins compartmentalized into small structures called nanodomains. Despite the asymmetric composition of both leaflets, coupling between the layers is surprisingly strong. This can be evidenced, for example, by recent experimental studies performed on phospholipid giant unilamellar vesicles showing that nanodomains formed in the outer layer are perfectly registered with those in the inner leaflet. Similarly, microscopic phase separation in one leaflet can induce phase separation in the opposing leaflet that would otherwise be homogeneous. In this review, we summarize the current theoretical and experimental knowledge that led to the current view that domains areirrespective of their size -commonly registered across the bilayer. Mechanisms inducing registration of nanodomains suggested by theory and calculations are discussed. Furthermore, domain coupling is evidenced by experimental studies based on the sparse number of methods that can resolve registered from independent nanodomains. Finally, implications that those findings using model membrane studies might have for cellular membranes are discussed.

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Fast flip-flop of cholesterol and fatty acids in membranes: implications for membrane transport proteins

Abstract: Knowledge of these properties provides a framework for understanding transport and metabolism of cholesterol and fatty acids and how their putative membrane and intracellular transporters might function.

Cited by 2 publications

References 27 publications

A lipid-free and insulin-supplemented medium supports De Novo fatty acid synthesis gene activation in melanoma cells

A lipid-free and insulin-supplemented medium supports De Novo fatty acid synthesis gene activation in melanoma cells

Interleaflet Coupling of Lipid Nanodomains – Insights From in vitro Systems

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