Fast‐GC–conventional quadrupole mass spectrometry in essential oil analysis

Abstract

This study reports on the compatibility of a conventional quadrupole MS (qMS) as detector for Fast-GC in terms of separation, identification, and quantitation when applied to the analysis of peppermint essential oil (e.o.), adopted as representative of this field. The influence of Fast-GC carried out on a 10 mx0.1 mm id narrow bore column with temperature programmes from 20 to 60 degrees C/min on the results of a qMS in total ion chromatograms (TIC) at different speeds (from 999 and 11 111 amu/s) and SIM modes was evaluated on ten differently abundant components characterizing peppermint e.o. Separation measure (S), peak capacity (n), and half height peak width were taken as separation parameters; match quality, number of scans per peak (NP), spectral skewing, and TIC area repeatability were used for identification. Quantitation was in SIM mode and NP, dwell time, SIM area repeatability and calibration curves, LOD, and LOQ of the selected components were measured. The results show that the peppermint e.o. markers can successfully be analysed qualitatively and quantitatively by F-GC-qMS up to temperature programmes of 60 degrees /min provided that a suitable scan speed is applied. Fast-GC-qMS reduces analysis time by a factor greater than ten and gives results that are qualitatively reliable and quantitatively comparable to those obtained by conventional GC-qMS.

Hydrodistillation and in situ microwave‐generated hydrodistillation of fresh and dried mint leaves: a comparison study

“…The mass spectrometric parameters were as follows; low mass 40, high mass 800, MS quad 150 °C, MS source 230 °C. Gas chromatography–mass spectrometry (GC‐MS) enables fast analysis, good separation and reliable quantitative and qualitative data 17…”

Essential oils and volatiles: sample preparation and analysis. A review.

“…suspected allergens in perfumes). The state of the art of the F-GC-qMS combination was exhaustively and critically reviewed by Mastovska et al [35] F-GC-qMS in EO analysis was also recently investigated in depth by Rubiolo et al [36] in a study dealing with separation, identification and quantitation of 10 components characteristic of peppermint EO. They showed that operating at a suitable scan speed in TIC mode (from 999 and 11111 amu/s) or at a suitable dwell time in SIM mode (0.5-100 ms), the results obtained with F-GC-qMS with a 10 m ¥ 0.1 mm i.d.…”

New trends in the analysis of the volatile fraction of matrices of vegetable origin: a short overview. A review.

“…Nowadays, GC-MS evolves towards speeding up the separation and detection steps, with approaches and instrumentation suitable for reducing the analysis time to a minimum while keeping good separation and producing reliable qualitative and quantitative results. [11] This goal has been achieved with short narrow-bore columns and modern GC instrumentation provided with automatic injectors, electronic flow control, ovens with high precision temperature and temperature rate controls, detectors with high sensitivity, electronic stability (low signal-tonoise ratio) and frequency of signal acquisition. An important contribution to fast GC in routine analysis was the method translation approach introduced by Klee and Blumberg, [12] which makes it possible to find the optimal separation/speed trade-off for a conventional GC method and to derive fast GC conditions from it automatically.…”