Fast growing aspens in the development of a plant micropropagation system based on plant-produced ethylene action

Žiauka, Jonas; Kuusienė, Sigutė; Šilininkas, M.

doi:10.1016/j.biombioe.2013.01.005

Cited by 9 publications

(6 citation statements)

References 39 publications

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“…In agreement with our observations, in the woody species Populus tremula, AVG treatments also inhibited shoot elongation, induction and development of buds and root formation; in turn, ACC and ETH treatments stimulated these parameters [72]. The positive effect of ethylene in shoot proliferation of P. tremula raises the prospect of micropropagation protocols based on the action of ethylene produced by the plant itself instead of exogenous hormone treatment, such as the use of small-volume vessels with gas exchange restriction [73].…”

Section: Inhibition Of Ethylene Biosynthesis and Perception During Sh...supporting

confidence: 90%

Ethylene Inhibition Reduces De Novo Shoot Organogenesis and Subsequent Plant Development from Leaf Explants of Solanum betaceum Cav.

Neves

Correia

Canhoto

2023

Plants

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In de novo shoot organogenesis (DNSO) plant cells develop into new shoots, without the need of an existing meristem. Generally, this process is triggered by wounding and specific growth regulators, such as auxins and cytokinins. Despite the potential significance of the plant hormone ethylene in DNSO, its effect in regeneration processes of woody species has not been thoroughly investigated. To address this gap, Solanum betaceum Cav. was used as an experimental model to explore the role of this hormone on DNSO and potentially extend the findings to other woody species. In this work it was shown that ethylene positively regulates DNSO from tamarillo leaf explants. Ethylene precursors ACC and ethephon stimulated shoot regeneration by increasing the number of buds and shoots regenerated. In contrast, the inhibition of ethylene biosynthesis or perception by AVG and AgNO3 decreased shoot regeneration. Organogenic callus induced in the presence of ethylene precursors showed an upregulated expression of the auxin efflux carrier gene PIN1, suggesting that ethylene may enhance shoot regeneration by affecting auxin distribution prior to shoot development. Additionally, it was found that the de novo shoot meristems induced in explants in which ethylene biosynthesis and perception was suppressed were unable to further develop into elongated shoots. Overall, these results imply that altering ethylene levels and perception could enhance shoot regeneration efficiency in tamarillo. Moreover, we offer insights into the possible molecular mechanisms involved in ethylene-induced shoot regeneration.

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Section: Inhibition Of Ethylene Biosynthesis and Perception During Sh...supporting

confidence: 90%

Ethylene Inhibition Reduces De Novo Shoot Organogenesis and Subsequent Plant Development from Leaf Explants of Solanum betaceum Cav.

Neves

Correia

Canhoto

2023

Plants

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“…Interestingly, during a similar study previously done on aspen (Populus tremula) explants [37], the conditions suitable for ethylene accumulation resulted in both enhanced shoot elongation and shoot proliferation simultaneously (during the initial subculture). The present results obtained with hybrid white poplar shoot culture suggest, however, that shoot elongation should be a secondary effect of sealing, possibly resulted from the increased activity of growth hormone gibberellin which is generally accepted by plant researchers to be responsible for shoot elongation [25], also in the genus Populus [10,36].…”

Section: Methodsmentioning

confidence: 53%

“…Since the increased accumulation of ethylene gas in tightly sealed plant culture vessels is a well-known side effect of in vitro culturing [2,4], it seemed promising to test the impact of additional sealing on plant development, while employing a hormone-free nutrient medium. Positive results (mainly, in terms of shoot proliferation) were obtained with aspens while culturing them in tightly sealed low-volume petri dishes, indicating that restricted gas exchange can substantially reduce the need for exogenous hormones [37].…”

Section: Introductionmentioning

confidence: 99%

Multiplication and growth of hybrid poplar(Populus alba × P. tremula)shoots on a hormone-free medium

Žiauka

Kuusienė

2014

Acta Biologica Hungarica

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the present study explored an alternative approach for poplar micropropagation, based on the restriction of gas exchange between inside and outside environments of culture vessel, rather than on the application of exogenous hormones. Apical and nodal stem segments (explants) excised from in vitro-developed shoots of hybrid white poplar (Populus alba L. × P. tremula L.) were incubated in either sealed (with Parafilm) or unsealed capped glass culture tubes (150 × 20 mm) on a hormone-free Woody Plant Medium. Shoot proliferation on apical explants was observed in sealed culture tubes but not in the unsealed ones; the difference between these two samples in respect of shoot number increased in the course of time and became threefold after three months of culture, with 3.2 ± 0.4 (mean ± SE) shoots per explant in the sealed tubes versus 1.1 ± 0.1 in the unsealed ones (for comparison, the mean shoot numbers on nodal explants were 2.4 ± 0.3 and 3.4 ± 0.4 in the unsealed and sealed culture tubes, respectively). Moreover, the shoots taken from the sealed culture tubes could be distinguished by superior shoot length, if compared to the shoots from the unsealed tubes, during the subsequent culture stage under uniform conditions.

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“…Fast-growing hybrid aspen (Populus tremula L. × Populus tremuloides Michx.) are currently of large interest in the Nordic and Baltic countries (Beuker 2000;Rytter and Stener 2005;Tullus et al 2007;Žiauka et al 2013;Jansons et al 2014). Hybrid aspen is considered to be a promising alternative in the boreal and hemi boreal zones, since it seems to combine fast growth and wood quality with high frost hardiness (Heräjärvi and Junkkonen 2006;Tullus et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Plasticity and stability of hybrid aspen clones in 14 field trials over Sweden, Finland and north-west Russia

Fedorkov

Stener

Pulkkinen

2021

Folia Forestalia Polonica

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The study aimed at estimating the genotype × environment (G × E) interaction, stability and plasticity parameters for height growth and survival of hybrid aspen (Populus tremula L. × Populus tremuloides Michx.) clones. A set of 17 hybrid aspen clones were studied at age four in 11 field trials (series 1) and a set of 12 clones were studied at age 12 in three field trials (series 2) in Sweden, Finland and north-west Russia. There was an intermediate and significant correlation between height ranks at four and 12 years, showing modest G × E interaction for tree height. The corresponding correlation for survival was weak and insignificant, indicating a stronger G × E interaction than for height. The stability parameter (S) for height showed large differences among clones. The plasticity index (PI) values for height ranged from 0.6 to 0.8 among clones, demonstrating high levels of phenotypic plasticity.

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Fast growing aspens in the development of a plant micropropagation system based on plant-produced ethylene action

Cited by 9 publications

References 39 publications

Ethylene Inhibition Reduces De Novo Shoot Organogenesis and Subsequent Plant Development from Leaf Explants of Solanum betaceum Cav.

Ethylene Inhibition Reduces De Novo Shoot Organogenesis and Subsequent Plant Development from Leaf Explants of Solanum betaceum Cav.

Multiplication and growth of hybrid poplar(Populus alba × P. tremula)shoots on a hormone-free medium

Plasticity and stability of hybrid aspen clones in 14 field trials over Sweden, Finland and north-west Russia

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