Fast in vivo bioluminescence tomography using a novel pure optical imaging technique

Abstract

Bioluminescence tomography (BLT) is a novel optical molecular imaging technique that advanced the conventional planar bioluminescence imaging (BLI) into a quanti¯able three-dimensional (3D) approach in preclinical living animal studies in oncology. In order to solve the inverse problem and reconstruct tumor lesions inside animal body accurately, the prior structural information is commonly obtained from X-ray computed tomography (CT). This strategy requires a complicated hybrid imaging system, extensive post imaging analysis and involvement of ionizing radiation. Moreover, the overall robustness highly depends on the fusion accuracy between the optical and structural information. Here, we present a pure optical bioluminescence tomographic (POBT) system and a novel BLT work°ow based on multi-view projection acquisition and 3D surface reconstruction. This method can reconstruct the 3D surface of an imaging subject based on a sparse set of planar white-light and bioluminescent images, so that the prior structural information can be o®ered for 3D tumor lesion reconstruction without the involvement of CT. The performance of this novel technique was evaluated through the comparison with a conventional dual-modality tomographic (DMT) system and a commercialized optical imaging system (IVIS Spectrum) using three breast cancer xenografts. The results revealed that the new technique o®ered comparable in vivo tomographic accuracy with the DMT system (P > 0:05) in much shorter data analysis time. It also o®ered signi¯cantly better accuracy comparing with the IVIS system (P < 0:04) without sacri¯cing too much time.

Multimodality system of x-ray and fluorescence based on Fourier single-pixel imaging for small animals

“…In-vivo imaging of small animals plays an important role in biomedical research including disease treatment and drug discovery. 1 – 3 Fluorescence imaging is a widely used modality with the advantage of noninvasive detecting, flexible fluorescent probes options, and providing molecular information. 4 However, a single fluorescence imaging modality lacks precise anatomical information and may lead to incorrect experimental results.…”