Fast identification of dermatophytes by <scp>MALDI</scp>‐<scp>TOF</scp>/<scp>MS</scp> using direct transfer of fungal cells on ground steel target plates

Cunha, Keith Cássia da; Riat, Arnaud; Normand, Anne‐Cécile; Bosshard, Philipp P.; Almeida, Margarete Teresa Gottardo de; Piarroux, Renaud; Schrenzel, Jacques; Fontao, Lionel

doi:10.1111/myc.12793

Cited by 26 publications

(21 citation statements)

References 23 publications

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“…[6][7][8]21,27 However, two studies recently assessed the ability to identify fungi by MALDI-TOF MS using a direct deposit method. 28,29 The rate of 78% of correct identification obtained for dermatophytes using the IDFP medium is satisfactory. L'Ollivier et al described in one review ten studies published between 2008 and 2015 showing accuracy of identification by MALDI-TOF MS ranging from 14% to 100% for dermatophytes.…”

Section: Discussionmentioning

confidence: 86%

Evaluation of the new Id‐Fungi plates from Conidia for MALDI‐TOF MS identification of filamentous fungi and comparison with conventional methods as identification tool for dermatophytes from nails, hair and skin samples

Sacheli¹,

Henri²,

Seidel

et al. 2020

Mycoses

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Objectives We first compare the efficiency of mould/dermatophyte identification by MALDI‐TOF MS using a new medium called Id‐Fungi plates (IDFP) from Conidia® and two different databases. For the second purpose, we evaluated a new version of the medium supplemented with cycloheximide, Id‐Fungi plates Plus (IDFPC) for the direct inoculation of nails, hair and skin samples and compared the efficiency of MALDI‐TOF MS identification of dermatophytes to classical methods based on culture and microscopy. Methods A total of 71 strains have been cultured IDFP and Sabouraud gentamicin plates (SGC2) and were identified by MALDI‐TOF MS. For the evaluation of the combination IDFPC/ MALDI‐TOF MS as a method of identification for dermatophytes, 428 samples of hair nails and skin were cultivated in parallel on IDFPC and Sabouraud + cycloheximide medium (SAB‐ACTI). Results For Aspergillus sp and non‐Aspergillus moulds, the best performances were obtained on IDFP after maximum 48‐h growth, following protein extraction. For dermatophytes, the best condition was using the IDFP at 72 hours, after extended direct deposit. Regarding the direct inoculation of nails, hair skin on IDFPC, 129/428 (30.1%) showed a positive culture against 150/428 (35%) on SAB‐ACTI medium. Among the 129 positive strains, the identification by MALDI‐TOF MS was correct for 92/129 (71.4%). Conclusion The IDFP allows the generation of better spectra by MALDI‐TOF MS compared to SGC2. It facilitates sampling and deposit. Regarding the use of IDFPC, this medium seems less sensitive than SAB‐ACTI but among positive strains, the rate of correct identification by MALDI‐TOF MS is satisfactory.

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Section: Discussionmentioning

confidence: 86%

Evaluation of the new Id‐Fungi plates from Conidia for MALDI‐TOF MS identification of filamentous fungi and comparison with conventional methods as identification tool for dermatophytes from nails, hair and skin samples

Sacheli¹,

Henri²,

Seidel

et al. 2020

Mycoses

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“…Use of a tube-based extraction step and a manufacturer database augmented with user-developed spectra were helpful for accurate species identification. Da Cunha et al assessed whether the direct transfer method can be used with dermatophytes [71]. They built their own library using the Bruker system and evaluated its performance with a panel of mass spectra produced with molecularly-identified isolates and, compared MALDI-ToF MS to morphology-based identification.…”

Section: Filamentous Fungimentioning

confidence: 99%

A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification

Patel

2019

JoF

116

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As a result of its being inexpensive, easy to perform, fast and accurate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is quickly becoming the standard means of bacterial identification from cultures in clinical microbiology laboratories. Its adoption for routine identification of yeasts and even dimorphic and filamentous fungi in cultures, while slower, is now being realized, with many of the same benefits as have been recognized on the bacterial side. In this review, the use of MALDI-ToF MS for identification of yeasts, and dimorphic and filamentous fungi grown in culture will be reviewed, with strengths and limitations addressed.

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“…An example is T. interdigitale , a species that is indistinguishable by micro- and macro-morphological characteristics from others of the T. mentagrophytes complex. In addition, various studies have focused on the identification by MALDI-TOF MS of dermatophytes isolated of clinical samples using different commercial platforms and pre-analytical methods, such as fungal growth time, culture media direct identification or from protein extracts [ 61 , 62 , 63 , 64 , 65 ]. Nevertheless, the identification at species-level with commercial libraries is low and variable between studies (14% and 62%) [ 63 , 65 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia)

Gómez-Velásquez¹,

Loaiza-Díaz²,

Hernández

et al. 2020

Microorganisms

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Identification of filamentous fungi by conventional phenotypic methods are time-consuming, and a correct identification at the species level is prone to errors. Therefore, a more accurate and faster time-to-results, and cost-effective technique, is required, such as the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). In this study, we describe the development of an in-house spectra library for the identification of filamentous fungi frequently isolated from patients with infections. An in-house spectra library was constructed using 14 reference strains grown in solid medium. Clinical isolates were identified either by the in-house spectra library or the Biotyper commercial library from Bruker Daltonics. Fungal identification was carried following the Biotyper’s established scores: ≤1.699: not reliably identified (NRI); 1.700–1.999: genus-level; ≥2.000: species-level. Clinical isolates were identified, with the in-house library, at species- and genus-level at 88.70% (55) and 3.22% (2), respectively. While 4.80% (3) was NRI and 3.22% (2) was discrepant concerning sequencing. On the contrary, identification up to species and genus-level with the commercial library was 44.44% (16) and 22.22% (8), respectively. NRI and the discrepancy was 30.55% (11) and 2.77% (1), respectively. For the reaming 26 isolates, 16 from Neoscytalidium dimidiatum and 10 from Sporothrix spp., respectively, the absence of spectrum and the specific spectra within the Sporothrix complex in the commercial library resulted in the inability to obtain an identification. In conclusion, the current results advocate the importance that each clinical microbiological laboratory needs to develop an ad hoc library associated with the MALDI-TOF MS fungal identification to overcome the limitations of the available commercial libraries.

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Fast identification of dermatophytes by MALDI‐TOF/MS using direct transfer of fungal cells on ground steel target plates

Cited by 26 publications

References 23 publications

Evaluation of the new Id‐Fungi plates from Conidia for MALDI‐TOF MS identification of filamentous fungi and comparison with conventional methods as identification tool for dermatophytes from nails, hair and skin samples

Evaluation of the new Id‐Fungi plates from Conidia for MALDI‐TOF MS identification of filamentous fungi and comparison with conventional methods as identification tool for dermatophytes from nails, hair and skin samples

A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification

Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia)

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