Fast impedimetric immunosensing of IgGs associated with peanut and hazelnut allergens

Ratiometric Fluorescence Aptasensor of Allergen Protein Based on Multivalent Aptamer-Encoded DNA Flowers as Fluorescence Resonance Energy Transfer Platform

“…10−12 So far, food allergies still cannot be completely cured, making stringent avoidance of allergen-containing foods is the preferred strategy to safeguard allergic individuals from anaphylaxis. 13,14 Thus, it is an urgent need to develop an efficient method to trace analysis of Ara h 1 in food for allergen labeling, dietary guidance, and ultimately preventing allergic incidents. 15−17 Up to now, a series of analysis strategies have been established for the qualitative or quantitative analysis of Ara h1, such as polymerase chain reaction (PCR), 12,18,19 loopmediated isothermal amplification (LAMP), 20,21 liquid chromatography−tandem mass spectrometry (LC−MS/MS), 22 fluorescence assay, 8 surface-enhanced Raman spectroscopy (SERS), 23 enzyme-linked immunosorbent assay (ELISA), 24 electrochemical method, 25 lateral flow assay (LFA), 24 surface plasmon resonance (SPR).…”

“…), fish, eggs, nuts, and wheat, are responsible for 90% of food allergic reactions. Therefore, the Food and Agriculture Organization of the United Nations (FAO)/World Health Organization (WHO) defined it as the most common food allergens. − Ara h1, the predominant allergenic protein in peanuts, is specifically acknowledged as a major concern owing to its distinctive trimeric structural configuration and widespread distribution characteristics. , The anaphylaxis triggered by Ara h1 is a typical food allergy reaction, which occurs both in children and adults, and even trace amounts of Ara h1 can cause severe allergic symptoms in allergic individuals. − So far, food allergies still cannot be completely cured, making stringent avoidance of allergen-containing foods is the preferred strategy to safeguard allergic individuals from anaphylaxis. , Thus, it is an urgent need to develop an efficient method to trace analysis of Ara h 1 in food for allergen labeling, dietary guidance, and ultimately preventing allergic incidents. − …”