Fast Isoelectric Focusing of Some Polymorphic Proteins and Enzymes in Miniaturized Gels Using an Automated System

Carracedo, Ángel; Andrade-Vide, C; Rodriguez-Calvo,; Montiel,; Lareu, M.V.

doi:10.1520/jfs12582j

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Cited by 3 publications

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“…IEF was performed with the automated Phastsystem (Phar macia) in miniaturized gels (pH 4-45) using the meth ods of Carracedo et al [6]. Before TF typing, samples were treated with ferrous ammonium sulfate, as de scribed by Constans et al [7].…”

Section: Methodsmentioning

confidence: 99%

PI and TF Subtypes in Chuetas (Majorcan Jews)

1992

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PI and TF subtypes were studied in a sample of 137 individuals of the Chueta population. In addition to the PI*M alleles, PI*S, PI*Z, and PI*F were observed in the PI system. In the TF system no TF*B or TF*D alleles were found. PI results were compared with those of some Jewish and non-Jewish populations. The relatively high frequency of PI*S is indicative of a substantial Spanish influence. There are no previous data available on TF*C subtypes in Jews. The very low TF*C3 frequency in Chuetas (lower than in Spain) indicates that this allele may be extremely rare or absent in other Jewish populations.

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Section: Methodsmentioning

confidence: 99%

PI and TF Subtypes in Chuetas (Majorcan Jews)

1992

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“…The validity and reliability of electrophoresis for the analyses of evidentiary material were affirmed after the effects of common contaminants on various forensically used genetic marker systems were studied (337). Separation of polymorphic proteins and enzymes on miniaturized polyacrylamide gels using an automated system has been reported (338). An aluminum template for agarose gels has been reported (339).…”

Section: Forensic Biochemistrymentioning

confidence: 99%