Fast isotopic separation of ¹⁰B and ¹¹B boric acid by capillary zone electrophoresis

Abstract

Fast isotopic separation of B and B boric acid by CZE was demonstrated. The BGE contained 25 mM phenylalanine and 5 mM putrescine (рН 8.95). The running conditions were +25 kV at 20°C with indirect photometric detection at 210 nm. Baseline separation was achieved in less than 9 min. RSD of migration times and corrected peak areas were less than 0.5 and 3%, respectively (n = 5). Linearity was demonstrated in the range 0.2-2 mM for B and 0.2-0.5 mM for B.

Identification of Isomers by Multidimensional Isotopic Shifts in High-Field Ion Mobility Spectra

“…Therefore, their separation properties theoretically differ in ways that (unlike in MS) depend on the structure. The LC − and CE − elution times for atomic and molecular species, including pure and substituted anilines similar to the chloroanilines studied here, , shift due to various heavy isotopes (D, 11 B, 15 N, 17 O, 18 O, 37 Cl, and 81 Br). With long gradients (up to 20 h), some nonisobaric isotopologues were fully resolved.…”

“…Here, the two Cl atoms and high 37 Cl fraction yield an abundance of ∼1% or more for species comprising 37 Cl 2 at the base monoisotopic peak (m/z 162) plus 4 and 5 Da (Table 1). As with the MCAs, >93% of the signal at any of those masses arises from the dominant stoichiometry, here with one or two Cl and/or one 13 C (but no 15 N or D). Hence, we can again ascribe the observed peaks to specific isotopologues.…”

Elemental Dependence of Structurally Specific Isotopic Shifts in High-Field Ion Mobility Spectra

“…Elucidating those comes to the forefront as crucial significance of isomers to biomedical functions of peptides, proteins, and metabolites gains appreciation. 7−10 However, chromatography, 11−13 electrophoresis, 14,15 and linear ion mobility spectrometry 16,17 (IMS) based on absolute ion mobility (K) at normally weak electric field (E) also discriminate nonisobaric isotopologues by mass without structural specificity.…”

“…The associated mass distribution fingerprints stoichiometry, which can be deduced using mass spectrometry (MS) with sufficient resolving power ( R MS ) and measurement accuracy, − but reveals nothing about the molecular geometries. Elucidating those comes to the forefront as crucial significance of isomers to biomedical functions of peptides, proteins, and metabolites gains appreciation. − However, chromatography, − electrophoresis, , and linear ion mobility spectrometry , (IMS) based on absolute ion mobility ( K ) at normally weak electric field ( E ) also discriminate nonisobaric isotopologues by mass without structural specificity.…”

Application of zero‐phase digital filtering for effective denoising of field asymmetric waveform ion mobility spectrometry signal

“…To effectively analyze the samples with ever‐increasing complexity, considerable efforts have been made to find a way to separate and characterize the mixture of analytes, which motivates the development of several ionic separation technologies such as ion chromatography, 1–3 electrophoresis, 4,5 and ion mobility spectrometry (IMS) 6,7 . Among these different separation techniques, IMS, developed early in the last century, relies on the ion mobility ( K ) differences among different ions in gas phase to separate them in a drift tube in which a uniform static electric field ( E ) is established in a nonreactive buffer gas environment 8–10 .…”